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Literature DB >> 3311166

Cation specificity for sugar substrates of the melibiose carrier in Escherichia coli.

Abstract

A study has been made of the sugar substrate specificities and the cation specificities of the melibiose transport system of Escherichia coli. The following beta-galactosides were found to be transported: lactose, L-arabinose-beta-D-galactoside, D-fructose-beta-D-galactoside, o- and p-nitrophenyl-beta-D-galactosides. These beta-galactosides were cotransported with Na+ but not with H+. The alpha-galactosides raffinose, melibiose and p-nitrophenyl-alpha-galactoside were transported with either H+ or Na+. Of the monosaccharides tested D-galactose could use either Na+ or H+ for cotransport whereas D-fucose, L-arabinose and D-galactosamine could use only Na+. The sugar specificity requirements for H+ cotransport are therefore more exacting than those for Na+ cotransport.

Entities: Chemical Gene Species

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Year: 1987 PMID： 3311166 DOI： 10.1016/0005-2736(87)90368-3

Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002

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Cited

25 in total

1. Arg-52 in the melibiose carrier of Escherichia coli is important for cation-coupled sugar transport and participates in an intrahelical salt bridge.

Authors: P J Franco; T H Wilson
Journal: J Bacteriol Date: 1999-10 Impact factor: 3.490

Review 2. Sodium ion cycle in bacterial pathogens: evidence from cross-genome comparisons.

Authors: C C Häse; N D Fedorova; M Y Galperin; P A Dibrov
Journal: Microbiol Mol Biol Rev Date: 2001-09 Impact factor: 11.056

3. Altered substrate selection of the melibiose transporter (MelY) of Enterobacter cloacae involving point mutations in Leu-88, Leu-91, and Ala-182 that confer enhanced maltose transport.

Authors: Steven G Shinnick; Stephanie A Perez; Manuel F Varela
Journal: J Bacteriol Date: 2003-06 Impact factor: 3.490

10. Characterization, expression, and mutation of the Lactococcus lactis galPMKTE genes, involved in galactose utilization via the Leloir pathway.

Authors: Benoît P Grossiord; Evert J Luesink; Elaine E Vaughan; Alain Arnaud; Willem M de Vos
Journal: J Bacteriol Date: 2003-02 Impact factor: 3.490

Cation specificity for sugar substrates of the melibiose carrier in Escherichia coli.

1. Arg-52 in the melibiose carrier of Escherichia coli is important for cation-coupled sugar transport and participates in an intrahelical salt bridge.

Review 2. Sodium ion cycle in bacterial pathogens: evidence from cross-genome comparisons.

3. Altered substrate selection of the melibiose transporter (MelY) of Enterobacter cloacae involving point mutations in Leu-88, Leu-91, and Ala-182 that confer enhanced maltose transport.

4. Effect of Detergents on Galactoside Binding by Melibiose Permeases.

5. Presence of multidrug-resistant enteric bacteria in dairy farm topsoil.

6. Asp-51 and Asp-120 are important for the transport function of the Escherichia coli melibiose carrier.

7. Reduced Na+ affinity increases turnover of Salmonella enterica serovar Typhimurium MelB.

8. Suppression of conformation-compromised mutants of Salmonella enterica serovar Typhimurium MelB.

9. Insights into the inhibitory mechanisms of the regulatory protein IIA(Glc) on melibiose permease activity.

10. Characterization, expression, and mutation of the Lactococcus lactis galPMKTE genes, involved in galactose utilization via the Leloir pathway.