Literature DB >> 33103664

Native detection of protein O-GlcNAcylation by gel electrophoresis.

Chuan Fu1, Daan M F van Aalten.   

Abstract

O-GlcNAcylation is an abundant and dynamic protein posttranslational modification (PTM), with crucial roles in metazoans. Studies of this modification are hampered by the lack of convenient methods for detecting native O-GlcNAcylation. Here, we describe a novel gel-based approach, Separation of O-GlcNAcylated Proteins by Polyacrylamide Gel Electrophoresis (SOPAGE), which enables detection of O-GlcNAc levels and dynamics.

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Year:  2020        PMID： 33103664      PMCID： PMC7611126          DOI： 10.1039/c9an02506e

Source DB:  PubMed          Journal:  Analyst        ISSN： 0003-2654            Impact factor:   4.616


  30 in total

1.  O-GlcNAc transferase catalyzes site-specific proteolysis of HCF-1.

Authors:  Francesca Capotosti; Sophie Guernier; Fabienne Lammers; Patrice Waridel; Yong Cai; Jingji Jin; Joan W Conaway; Ronald C Conaway; Winship Herr
Journal:  Cell       Date:  2011-02-04       Impact factor: 41.582

2.  Structural insights into the mechanism and inhibition of eukaryotic O-GlcNAc hydrolysis.

Authors:  Francesco V Rao; Helge C Dorfmueller; Fabrizio Villa; Matthew Allwood; Ian M Eggleston; Daan M F van Aalten
Journal:  EMBO J       Date:  2006-03-16       Impact factor: 11.598

3.  O-GlcNAc cycling mutants modulate proteotoxicity in Caenorhabditis elegans models of human neurodegenerative diseases.

Authors:  Peng Wang; Brooke D Lazarus; Michele E Forsythe; Dona C Love; Michael W Krause; John A Hanover
Journal:  Proc Natl Acad Sci U S A       Date:  2012-09-17       Impact factor: 11.205

Review 4.  Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins.

Authors:  Gerald W Hart; Michael P Housley; Chad Slawson
Journal:  Nature       Date:  2007-04-26       Impact factor: 49.962

5.  A mutant O-GlcNAcase as a probe to reveal global dynamics of protein O-GlcNAcylation during Drosophila embryonic development.

Authors:  Daniel Mariappa; Nithya Selvan; Vladimir Borodkin; Jana Alonso; Andrew T Ferenbach; Claire Shepherd; Iva Hopkins Navratilova; Daan M F vanAalten
Journal:  Biochem J       Date:  2015-07-14       Impact factor: 3.857

6.  A mutant O-GlcNAcase enriches Drosophila developmental regulators.

Authors:  Nithya Selvan; Ritchie Williamson; Daniel Mariappa; David G Campbell; Robert Gourlay; Andrew T Ferenbach; Tonia Aristotelous; Iva Hopkins-Navratilova; Matthias Trost; Daan M F van Aalten
Journal:  Nat Chem Biol       Date:  2017-06-12       Impact factor: 15.040

7.  Chemical Modulation of Protein O-GlcNAcylation via OGT Inhibition Promotes Human Neural Cell Differentiation.

Authors:  Lissette M Andres; Ian W Blong; Angela C Evans; Neil G Rumachik; Teppei Yamaguchi; Nam D Pham; Pamela Thompson; Jennifer J Kohler; Carolyn R Bertozzi
Journal:  ACS Chem Biol       Date:  2017-06-19       Impact factor: 5.100

8.  Microfluidic Western blotting of low-molecular-mass proteins.

Authors:  Rachel E Gerver; Amy E Herr
Journal:  Anal Chem       Date:  2014-10-15       Impact factor: 6.986

9.  Mutations in N-acetylglucosamine (O-GlcNAc) transferase in patients with X-linked intellectual disability.

Authors:  Anke P Willems; Mehmet Gundogdu; Marlies J E Kempers; Jacques C Giltay; Rolph Pfundt; Martin Elferink; Bettina F Loza; Joris Fuijkschot; Andrew T Ferenbach; Koen L I van Gassen; Daan M F van Aalten; Dirk J Lefeber
Journal:  J Biol Chem       Date:  2017-06-05       Impact factor: 5.157

10.  Tau post-translational modifications in wild-type and human amyloid precursor protein transgenic mice.

Authors:  Meaghan Morris; Giselle M Knudsen; Sumihiro Maeda; Jonathan C Trinidad; Alexandra Ioanoviciu; Alma L Burlingame; Lennart Mucke
Journal:  Nat Neurosci       Date:  2015-07-20       Impact factor: 24.884
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