Literature DB >> 33099111

Generation of the induced pluripotent stem cell line UHOMi002-A from peripheral blood mononuclear cells of a healthy male donor.

Mathieu Fieldes1, Engi Ahmed2, Chloé Bourguignon1, Joffrey Mianné1, Mélanie Martin3, Cécile Arnould3, Isabelle Vachier4, Said Assou1, John De Vos5, Arnaud Bourdin6.   

Abstract

Human pluripotent stem cells (hiPSC) are highly valuable tools to model lung development and chronic bronchial diseases. We generated a hiPSC line from a highly characterized 40-year-old healthy male nonsmoking donor. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using integration-free Sendai Virus. The cell line had normal karyotype, expressed pluripotency hallmarks, and differentiated into the three primary germ layers. The reported UHOMi002-A iPSC line may be used as a control to model lung development, study human chronic bronchial diseases and drug testing.
Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

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Year:  2020        PMID: 33099111     DOI: 10.1016/j.scr.2020.102037

Source DB:  PubMed          Journal:  Stem Cell Res        ISSN: 1873-5061            Impact factor:   2.020


  2 in total

1.  CRISPR/Cas9-mediated gene knockout and interallelic gene conversion in human induced pluripotent stem cells using non-integrative bacteriophage-chimeric retrovirus-like particles.

Authors:  Joffrey Mianné; Amel Nasri; Chloé Nguyen Van; Chloé Bourguignon; Mathieu Fieldès; Engi Ahmed; Christine Duthoit; Nicolas Martin; Hugues Parrinello; Anaïs Louis; Alexandra Iché; Régis Gayon; Florine Samain; Lucille Lamouroux; Pascale Bouillé; Arnaud Bourdin; Said Assou; John De Vos
Journal:  BMC Biol       Date:  2022-01-07       Impact factor: 7.431

2.  Differentiation of Human Induced Pluripotent Stem Cells from Patients with Severe COPD into Functional Airway Epithelium.

Authors:  Engi Ahmed; Mathieu Fieldes; Chloé Bourguignon; Joffrey Mianné; Aurélie Petit; Myriam Jory; Chantal Cazevieille; Hassan Boukhaddaoui; James P Garnett; Christophe Hirtz; Gladys Massiera; Isabelle Vachier; Said Assou; Arnaud Bourdin; John De Vos
Journal:  Cells       Date:  2022-08-05       Impact factor: 7.666

  2 in total

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