The potential role of PSMA6 in modulating fat deposition in pigs by promoting preadipocyte proliferation and differentiation.

To investigate whether the proteasome subunit alpha 6 (PSMA6) gene has an effect on fat deposition, the gene expression profile was first detected in Berkshire pigs and Jinhua pigs (JHP). The results demonstrated that significantly higher levels of mRNA expression were identified in adipose tissues and the liver. Interestingly, when compared to the longissimus dorsi muscle (LDM) in each breed, it was discovered that the expression levels of the PSMA6 gene in these tissues of JHP were considerably higher than those in Berkshire pigs. Meantime, some significant correlations of PSMA6 mRNA expression in lipid metabolism-related tissues such as the liver and LDM with the marbling score, as well as the content of intramuscular fat (IMF), in pigs were found by correlation coefficient analysis. To further explore the effects of PSMA6 expression on fat deposition, we performed PSMA6 overexpression in 3T3-L1 cells via Lentivirus infection. Our results indicated that PSMA6 could promote cell proliferation and accelerate cell division. It was also found that the transcription factors CCAAT/enhancer-binding protein alpha (CEBPA) and peroxisome proliferator-activated receptor gamma (PPARG), as well as the key genes related to adipogenesis, were upregulated, while the genes related to fat oxidation were significantly downregulated, which indicated that the PSMA6 gene could stimulate the differentiation of preadipocytes.