Sharmila Pramanik 1 , Eric Yang 2 , Wendy Wu 1 Show Affiliations »
Abstract
OBJECTIVES: Recent research shows that most high grade ovarian cancer (OC) originates from the fallopian tube (FT). Cytologic evaluation of FT cells may enable early detection of OC. MATERIAL AND METHODS: This was a prospective study with patients enrolled from 3 centers (October 2016- August 2017). Forty-two women undergoing salpingo-oophorectomy for a pelvic mass suspicious for malignancy or undergoing risk-reducing surgery for BRCA mutations were included in the study. At the time of scheduled surgery, a novel catheter was used to collect FT cells through hysteroscopy. A pathologist blinded to surgical or pathologic findings evaluated FT cytology, and results were compared to pathology. RESULTS: Of the 61 samples collected, 72% (44/61) met the adequacy criteria (≥5 clusters of cells with 20 cells in each cluster). Cytology classification criteria were established and applied to adequate samples. Forty-four samples were benign with mixed population of cells with round, oval, and spindled nuclei; 2-dimensional clusters; columnar cell configuration; flat sheets; cilia presence; no/mild nuclear pleomorphism; no nuclear membrane irregularities; and no nucleoli. Five samples had benign features with reactive nuclear and cytoplasmic changes and/or background inflammation, which were categorized as "reactive atypia." Two malignant samples had features of 3-dimensional (3D) clusters, loss of mixed population of cells; increased nuclear/cytoplasmic ratio; nuclear membrane irregularity and nucleoli presence. Three samples with some but not all of malignant features were categorized as "neoplastic" (anisonucleosis; small nucleoli and features suggestive of 3D clusters). Malignant/ neoplastic samples were labeled as "Positive" (n = 5) while benign/reactive samples were labeled as "Negative" (n = 39). A high concordance rate (95%, 42/44) was observed between cytology results and histology. CONCLUSIONS: We characterized cytologic features for pathologically distinct FT samples collected in vivo using a novel catheter and demonstrated its value in detecting OC. ©2020 Cytopathology Foundation Inc, Published by Scientific Scholar.
OBJECTIVES: Recent research shows that most high grade ovarian cancer (OC) originates from the fallopian tube (FT). Cytologic evaluation of FT cells may enable early detection of OC. MATERIAL AND METHODS: This was a prospective study with patients enrolled from 3 centers (October 2016- August 2017). Forty-two women undergoing salpingo-oophorectomy for a pelvic mass suspicious for malignancy or undergoing risk-reducing surgery for BRCA mutations were included in the study. At the time of scheduled surgery, a novel catheter was used to collect FT cells through hysteroscopy. A pathologist blinded to surgical or pathologic findings evaluated FT cytology, and results were compared to pathology. RESULTS: Of the 61 samples collected, 72% (44/61) met the adequacy criteria (≥5 clusters of cells with 20 cells in each cluster). Cytology classification criteria were established and applied to adequate samples. Forty-four samples were benign with mixed population of cells with round, oval, and spindled nuclei; 2-dimensional clusters; columnar cell configuration; flat sheets; cilia presence; no/mild nuclear pleomorphism; no nuclear membrane irregularities; and no nucleoli. Five samples had benign features with reactive nuclear and cytoplasmic changes and/or background inflammation, which were categorized as "reactive atypia." Two malignant samples had features of 3-dimensional (3D) clusters, loss of mixed population of cells; increased nuclear/cytoplasmic ratio; nuclear membrane irregularity and nucleoli presence. Three samples with some but not all of malignant features were categorized as "neoplastic" (anisonucleosis; small nucleoli and features suggestive of 3D clusters). Malignant/ neoplastic samples were labeled as "Positive" (n = 5) while benign/reactive samples were labeled as "Negative" (n = 39). A high concordance rate (95%, 42/44) was observed between cytology results and histology. CONCLUSIONS: We characterized cytologic features for pathologically distinct FT samples collected in vivo using a novel catheter and demonstrated its value in detecting OC. ©2020 Cytopathology Foundation Inc, Published by Scientific Scholar.
Entities: Chemical
Keywords:
BRCA; Cytuity; Detection; Hysteroscopy; Ovarian cancer
Year: 2020
PMID： 33093855 PMCID： PMC7568227 DOI： 10.25259/CytoJournal_7_2020
Source DB: PubMed Journal: Cytojournal ISSN： 1742-6413 Impact factor: 2.091