| Literature DB >> 33092596 |
Chenying Lu1,2, Shiji Fang1,2, Qiaoyou Weng1,2, Xiuling Lv1,2, Miaomiao Meng1,2, Jinyu Zhu1,2, Liyun Zheng1,2, Yumin Hu1,2, Yang Gao1,2, Xulu Wu1,2, Jianting Mao1,2, Bufu Tang1,2, Zhongwei Zhao1,2, Li Huang3, Jiansong Ji4,5.
Abstract
An amendment to this paper has been published and can be accessed via the original article.Entities:
Year: 2020 PMID: 33092596 PMCID: PMC7580011 DOI: 10.1186/s12964-020-00674-y
Source DB: PubMed Journal: Cell Commun Signal ISSN: 1478-811X Impact factor: 5.712
Fig. 3OPN promotes the Warburg effect in HCC cells. a The knockdown efficiency of OPN in HCC-LM3 cells was measured by Western blotting and ELISA. b Effects of OPN knockdown on the glucose uptake and lactate production in HCC-LM3 cells (n = 3). c The extracellular acidification rate (ECAR) in sh-OPN and sh-Ctrl HCC-LM3 cells was measured by Seahorse analyzer (n = 5). d Effects of OPN blockade on the glucose uptake and lactate production in HCC-LM3 cells (n = 3). e The overexpression efficiency of OPN in NIH3T3 cells and MEFs was measured by Western blotting. f Effects of OPN overexpression on the glucose uptake and lactate production in NIH3T3 cells and MEFs (n = 3). g Effects of OPN overexpression on ECAR in NIH3T3 cells and MEFs were measured by Seahorse analyzer (n = 5). *P < 0.05 and **P < 0.01
Fig. 8Expression pattern of OPN in clinical samples. a The expression of glycolytic genes in human HCC tissue samples with high OPN (n = 10) and low OPN (n = 20) expression was analyzed by real-time qPCR. b Representative photographs of OPN expression in HCC tumor tissues; scale bar: 50 μm. The correlation between OPN expression and the SUVmax value was analyzed. *P < 0.05 and **P < 0.01