Haiam Mohamed Mahmoud Farrag1, Fatma Abdel Aziz Mohamed Mostafa2, Mona Embarek Mohamed3, Enas Abdelhameed Mahmoud Huseein4. 1. Department of Parasitology, Faculty of Medicine, Assiut University, Assiut, Egypt; Faculty of Applied Medical Sciences, Shaqra University, Saudi Arabia. Electronic address: hayammahmoud@su.edu.sa. 2. Department of Botany and Microbiology, Faculty of Science, Cairo University, Cairo, Egypt. 3. Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt. 4. Department of Parasitology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Abstract
BACKGROUND: Fungi represent an interesting candidate for the synthesis of nanoparticles. The biosynthesis of silver nanoparticles (AgNPs) has many industrial and biomedical indications. We aimed in this work to biologically synthesize silver nanoparticles using Aspergillus niger and to evaluate its effect against the newly identified Allovahlkampfia spelaea that causes resistant human keratitis. MATERIAL AND METHODS: Aspergillus niger (soil isolate) was treated with silver nitrate to produce silver nanoparticles. AgNPs were characterized by Ultraviolet-Visible Spectroscopy, Transmission Electron Microscopy, and Fourier Transform Infrared Spectroscopy. The effect of the synthesized nanoparticles against Allovahlkampfia spelaea growth, encystation, excystation, and toxicity in host cells was evaluated. RESULTS: AgNPs exhibited significant inhibition of Allovahlkampfia spelaea viability and growth of both trophozoites and cysts, with a reduction of amoebic cytotoxic activity in host cells. CONCLUSION: AgNPs may give a promising future to the treatment of Allovahlkampfia spelaea infections in humans.
BACKGROUND: Fungi represent an interesting candidate for the synthesis of nanoparticles. The biosynthesis of silver nanoparticles (AgNPs) has many industrial and biomedical indications. We aimed in this work to biologically synthesize silver nanoparticles using Aspergillus niger and to evaluate its effect against the newly identified Allovahlkampfia spelaea that causes resistant humankeratitis. MATERIAL AND METHODS:Aspergillus niger (soil isolate) was treated with silver nitrate to produce silver nanoparticles. AgNPs were characterized by Ultraviolet-Visible Spectroscopy, Transmission Electron Microscopy, and Fourier Transform Infrared Spectroscopy. The effect of the synthesized nanoparticles against Allovahlkampfia spelaea growth, encystation, excystation, and toxicity in host cells was evaluated. RESULTS: AgNPs exhibited significant inhibition of Allovahlkampfia spelaea viability and growth of both trophozoites and cysts, with a reduction of amoebic cytotoxic activity in host cells. CONCLUSION: AgNPs may give a promising future to the treatment of Allovahlkampfia spelaea infections in humans.
Authors: Parameshwar Jakinala; Nageshwar Lingampally; Bee Hameeda; R Z Sayyed; Yahya Khan M; Elsayed Ahmed Elsayed; Hesham El Enshasy Journal: PLoS One Date: 2021-03-18 Impact factor: 3.240
Authors: Ali Es-Haghi; Mohammad Ehsan Taghavizadeh Yazdi; Mohammad Sharifalhoseini; Mohsen Baghani; Ehsan Yousefi; Abbas Rahdar; Francesco Baino Journal: Biomimetics (Basel) Date: 2021-05-27