Literature DB >> 3308918

Studies of tektin filaments from flagellar microtubules by immunoelectron microscopy.

W B Amos1, L A Amos, R W Linck.   

Abstract

Chemically resistant 2-3 nm filaments with a high alpha-helical content, isolated from sea-urchin sperm flagellar doublet microtubules, consist of proteins that have been named tektins. Polyclonal affinity-purified antibodies to tektins labelled sperm tails all along their lengths, as shown by indirect immunofluorescence microscopy, provided the specimens were not too well fixed. Results obtained for unfixed specimens studied by immunoelectron microscopy suggested the tektins are normally masked by tubulin. A monoclonal anti-tektin antibody labelled bare tektin filaments at longitudinal intervals of approximately 48 nm, which fits in well with the 96 nm longitudinal repeat of axonemes. We discuss a possible scheme for the regular interaction of tubulin monomers with an alpha-helical coiled coil.

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Year:  1986        PMID： 3308918     DOI： 10.1242/jcs.1986.supplement_5.4

Source DB:  PubMed          Journal:  J Cell Sci Suppl        ISSN： 0269-3518


  8 in total

1.  Primary structure of tektin A1: comparison with intermediate-filament proteins and a model for its association with tubulin.

Authors:  J M Norrander; L A Amos; R W Linck
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205

2.  The Rib43a protein is associated with forming the specialized protofilament ribbons of flagellar microtubules in Chlamydomonas.

Authors:  J M Norrander; A M deCathelineau; J A Brown; M E Porter; R W Linck
Journal:  Mol Biol Cell       Date:  2000-01       Impact factor: 4.138

3.  Cep57 protein is required for cytokinesis by facilitating central spindle microtubule organization.

Authors:  Runsheng He; Qixi Wu; Haining Zhou; Ning Huang; Jianguo Chen; Junlin Teng
Journal:  J Biol Chem       Date:  2013-04-08       Impact factor: 5.157

4.  Evidence for tektins in centrioles and axonemal microtubules.

Authors:  W Steffen; R W Linck
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205

5.  Mice deficient in the axonemal protein Tektin-t exhibit male infertility and immotile-cilium syndrome due to impaired inner arm dynein function.

Authors:  Hiromitsu Tanaka; Naoko Iguchi; Yoshiro Toyama; Kouichi Kitamura; Tohru Takahashi; Kazuhiro Kaseda; Mamiko Maekawa; Yoshitake Nishimune
Journal:  Mol Cell Biol       Date:  2004-09       Impact factor: 4.272

6.  Production of recombinant human tektin 1, 2, and 4 and in vitro assembly of human tektin 1.

Authors:  M S Budamagunta; F Guo; N Sun; B Shibata; P G FitzGerald; J C Voss; J F Hess
Journal:  Cytoskeleton (Hoboken)       Date:  2017-12-02

7.  Insights into the structure and function of ciliary and flagellar doublet microtubules: tektins, Ca2+-binding proteins, and stable protofilaments.

Authors:  Richard Linck; Xiaofeng Fu; Jianfeng Lin; Christna Ouch; Alexandra Schefter; Walter Steffen; Peter Warren; Daniela Nicastro
Journal:  J Biol Chem       Date:  2014-05-02       Impact factor: 5.157

8.  Biochemical characterization of tektins from sperm flagellar doublet microtubules.

Authors:  R W Linck; R E Stephens
Journal:  J Cell Biol       Date:  1987-04       Impact factor: 10.539
  8 in total

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