| Literature DB >> 3308638 |
J Gómez-Márquez1, M Freire, F Segade.
Abstract
We report a simple, rapid and reliable procedure for large-scale purification of plasmid DNA from non-amplified bacterial cultures. It is a modification of the boiling method of Holmes and Quigley [Anal. Biochem. 114 (1981) 193-197] and involves gel-filtration chromatography using Sephacryl S-1000 for final purification of plasmid DNA. This method does not require CsCl gradients and the recovered plasmids are free of RNA and chromosomal DNA, are supercoiled, retain their biological activity, and are suitable for restriction analysis.Entities:
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Year: 1987 PMID： 3308638 DOI： 10.1016/0378-1119(87)90494-x
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688