Ayman S Bannaga1,2, Frank Kvasnik3, Krishna Persaud4, Ramesh P Arasaradnam1,2,5,6. 1. Department of Gastroenterology and Hepatology, University Hospital Coventry and Warwickshire NHS Trust, Clifford Bridge Road, Coventry CV2 2DX, United Kingdom. 2. Warwick Medical School, Gibbet Hill Campus, Medical School Building, Coventry CV4 7HL, United Kingdom. 3. SensAm Limited, Unit1 Green Fold Court, Green Fold Way, Leigh WN7 3XJ, United Kingdom. 4. School of Chemical Engineering & Analytical Science, University of Manchester, Manchester M1 3AL, United Kingdom. 5. Faculty of Health & Life Sciences, Coventry University, Priory St, Coventry CV1 5FB, United Kingdom. 6. School of Biological Sciences, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom.
Abstract
BACKGROUND: In the human body, volatile organic compounds (VOCs) are produced by different tissues then secreted in different body fluids and subsequently excreted. Here we explore a non-invasive method for the detection of liver, prostate and bladder cancers. METHODS: We recruited 140 cases. There were 31 hepatocellular carcinomas (HCC), 62 prostate carcinomas, 29 bladder carcinomas and 18 non-cancer cases. Male to female ratio was 5:1 and mean age was 72 years. Urinary VOCs were detected by applying solid-phase microextraction (SPME) technique. RESULTS: The sensitivity for detection of HCC with normal alpha fetoprotein (AFP) was 68% (SE 0.06, 95% CI 0.54 to 0.81 and P < 0.005). The VOCs sensitivity in the detection of HCC cases with raised AFP was 83%. (SE 0.05, 95% CI 0.73 to 0.93 and P < 0.0001). The VOCs sensitivity for prostate cancer detection was 70% (SE 0.049, 95% CI 0.60 to 0.79 and P < 0.0002) and sensitivity for bladder cancer detection was 81% (SE 0.052, 95% CI 0.70 to 0.91 and P < 0.0001). CONCLUSIONS: SPME urinary VOCs analysis was able to differentiate between controls and each of hepatocellular, prostate and bladder cancers. This suggests that urinary VOCs are cancer specific and could potentially be used as a diagnostic method.
BACKGROUND: In the human body, volatile organic compounds (VOCs) are produced by different tissues then secreted in different body fluids and subsequently excreted. Here we explore a non-invasive method for the detection of liver, prostate and bladder cancers. METHODS: We recruited 140 cases. There were 31 hepatocellular carcinomas (HCC), 62 prostate carcinomas, 29 bladder carcinomas and 18 non-cancer cases. Male to female ratio was 5:1 and mean age was 72 years. Urinary VOCs were detected by applying solid-phase microextraction (SPME) technique. RESULTS: The sensitivity for detection of HCC with normal alpha fetoprotein (AFP) was 68% (SE 0.06, 95% CI 0.54 to 0.81 and P < 0.005). The VOCs sensitivity in the detection of HCC cases with raised AFP was 83%. (SE 0.05, 95% CI 0.73 to 0.93 and P < 0.0001). The VOCs sensitivity for prostate cancer detection was 70% (SE 0.049, 95% CI 0.60 to 0.79 and P < 0.0002) and sensitivity for bladder cancer detection was 81% (SE 0.052, 95% CI 0.70 to 0.91 and P < 0.0001). CONCLUSIONS: SPME urinary VOCs analysis was able to differentiate between controls and each of hepatocellular, prostate and bladder cancers. This suggests that urinary VOCs are cancer specific and could potentially be used as a diagnostic method.
Authors: Lauren Lett; Michael George; Rachael Slater; Ben De Lacy Costello; Norman Ratcliffe; Marta García-Fiñana; Henry Lazarowicz; Chris Probert Journal: Br J Cancer Date: 2022-03-29 Impact factor: 9.075
Authors: Christopher Bradley; Siew Wan Hee; Lazaros Andronis; Krishna Persaud; Mark A Hull; John Todd; Sian Taylor-Phillips; Steve Smith; Rachel Constable; Norman Waugh; Ramesh P Arasaradnam Journal: BMJ Open Date: 2022-03-30 Impact factor: 2.692