Khadijeh Nematdoost1,2, Keyhan Ashrafi2, Bijan Majidi-Shad2, Eshrat Beigom Kia3, Arash Zeinali4, Meysam Sharifdini5,6. 1. Cellular and Molecular Research Center, Guilan University of Medical Sciences, Rasht, Iran. 2. Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran. 3. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 4. Veterinary Head Office of Guilan Province, Rasht, Iran. 5. Cellular and Molecular Research Center, Guilan University of Medical Sciences, Rasht, Iran. sharifdini@gums.ac.ir. 6. Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran. sharifdini@gums.ac.ir.
Abstract
PURPOSE: Identification of different genotypes of echinococcal cyst in various domestic herbivores and humans within the target area was the principal aim of the present study, performed using sequence data of cox1 and nad1 mitochondrial genes. METHODS: A total of 57 cystic echinococcosis (CE) cysts were isolated from indigenous livestock including 45 cattle, 9 sheep and 3 goats from several slaughterhouses in Guilan Province. Moreover, 12 formalin-fixed paraffin-embedded (FFPE) CE cyst tissues from humans were also included, obtained from the archives of several hospitals in Rasht, the capital of Guilan. Genetic sequencing was conducted using mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes. RESULTS: Our results found that E. granulosus sensu stricto (s.s.) and E. ortleppi were present in 92.7% and 7.2% isolates, respectively. E. granulosus s.s. (genotypes G1 and G3) and E. ortleppi were isolated from various livestock whereas all CE cysts isolated from humans were E. granulosus s.s. G1 genotype. CONCLUSION: We found that E. granulosus s.s. G1 was the predominant genotype within the study region. This is the first study to report E. ortleppi in cattle in Iran.
PURPOSE: Identification of different genotypes of echinococcal cyst in various domestic herbivores and humans within the target area was the principal aim of the present study, performed using sequence data of cox1 and nad1 mitochondrial genes. METHODS: A total of 57 cystic echinococcosis (CE) cysts were isolated from indigenous livestock including 45 cattle, 9 sheep and 3 goats from several slaughterhouses in Guilan Province. Moreover, 12 formalin-fixed paraffin-embedded (FFPE) CE cyst tissues from humans were also included, obtained from the archives of several hospitals in Rasht, the capital of Guilan. Genetic sequencing was conducted using mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes. RESULTS: Our results found that E. granulosus sensu stricto (s.s.) and E. ortleppi were present in 92.7% and 7.2% isolates, respectively. E. granulosus s.s. (genotypes G1 and G3) and E. ortleppi were isolated from various livestock whereas all CE cysts isolated from humans were E. granulosus s.s. G1 genotype. CONCLUSION: We found that E. granulosus s.s. G1 was the predominant genotype within the study region. This is the first study to report E. ortleppi in cattle in Iran.
Authors: P Deplazes; L Rinaldi; C A Alvarez Rojas; P R Torgerson; M F Harandi; T Romig; D Antolova; J M Schurer; S Lahmar; G Cringoli; J Magambo; R C A Thompson; E J Jenkins Journal: Adv Parasitol Date: 2017-01-20 Impact factor: 3.870
Authors: Marion Hüttner; Minoru Nakao; Torsten Wassermann; Ludwig Siefert; Joop D F Boomker; Anke Dinkel; Yasuhito Sako; Ute Mackenstedt; Thomas Romig; Akira Ito Journal: Int J Parasitol Date: 2007-11-04 Impact factor: 3.981
Authors: U Saarma; I Jõgisalu; E Moks; A Varcasia; A Lavikainen; A Oksanen; S Simsek; V Andresiuk; G Denegri; L M González; E Ferrer; T Gárate; L Rinaldi; P Maravilla Journal: Parasitology Date: 2009-01-21 Impact factor: 3.234