The distribution of p29 protein in normal human skin.

In order to identify specific oestrogen-sensitive structures, normal human skin was examined for the binding of the ER D5 antibody which is associated with p29, a 29 kilodalton protein found in the cytoplasm of normal oestrogen-sensitive cells. Strong and specific staining was seen in the epidermis, with a gradient showing the most intense staining in the granular layer. Similar positive staining was seen in the hair follicles and sebaceous glands. Variable staining was seen in the eccrine glands and vessels. These findings demonstrate p29 to be present in these structures, and hence that oestrogens may exert a specific effect on these tissues.