Yue Qiu1, Junwen Yang2, Yiping Chen3, Jinhong Yang4, Qingxiong Zhu5, Chunhui Zhu5, Shuangjie Li6, Jing Liu7, Chaomin Wan8, Yu Zhu8, Minxia Chen9, Yi Xu9, Jianning Tong10, Rui Li10, Qingwen Shan11, Daojiong Lin12, Shouye Wu12, Zhiqiang Zhuo13, Caihong Wang13, Shiyong Zhao14, Zhenghong Qi14, Xiaofeng Sun15, Bieerding Maihebuba15, Chunmei Jia16, Huiling Gao16, Yibing Cheng17, Mei Zeng18. 1. Department of Infectious Diseases, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai, 201102, China. 2. Department of Microbiology Laboratory, Children's Hospital Affiliated to Zhengzhou University (Henan Children's Hospital), Zhengzhou, China. 3. Department of Pediatrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China. 4. Department of Microbiology Laboratory, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China. 5. Department of Infection Diseases, Children's hospital of Jiangxi Province, Nanchang, China. 6. Department of Hepatology, Hunan Children's Hospital, Changsha, China. 7. Department of Infection Diseases, Hunan Children's Hospital, Changsha, China. 8. Department of Pediatrics, Sichuan University West China Second Hospital, Chengdu, China. 9. Department of Infections Disease, Guangzhou Women and Children's Medical Center, Guangzhou, China. 10. Department of Pediatric, Gastroenterology and Infectious Diseases, Qingdao Women and Children's Hospital, Qingdao, China. 11. Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China. 12. Department of infectious diseases, Hainan Women and Children's Medical Center, Haikou, China. 13. Department of infectious diseases, Xiamen Children's Hospital, Xiamen, China. 14. Department of Infectious Diseases, Hangzhou Children's Hospital, Hangzhou, China. 15. Department of Infectious Diseases, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China. 16. Department of Pharmacy, The fourth Hospital of Baotou, Baotou, China. 17. Department of Emergency, Henan Children's Hospital Affiliated to Zhengzhou University, 33, Longhu Outer Ring East Road, Zhengzhou, 450018, China. 13703829317@163.com. 18. Department of Infectious Diseases, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai, 201102, China. zengmeigao@aliyun.com.
Abstract
OBJECTIVES: This study aimed to investigate the microbiological profiles and antimicrobial resistance patterns of bloodstream pathogens in Chinese children. METHODS: This retrospective study was conducted at 13 tertiary hospitals in China during 2016-2018. The first bloodstream isolates of the same species from one pediatric patient < 18 years were included to this study for analysis. Antimicrobial susceptibility testing was determined based on minimum inhibitory concentrations or Kirby-Bauer disk diffusion methods according to the 2018 Clinical and Laboratory Standards Institute guidelines. RESULTS: Overall, 9345 nonduplicate bloodstream isolates were collected. Top 10 pathogens included Coagulase-negative staphylococcus (CoNS) (44.4%), Escherichia coli (10.2%), Klebsiella pneumoniae (5.9%), Staphylococcus aureus (5.0%), Streptococcus pneumoniae (4.9%), Pseudomonas aeruginosa(2.8%), Enterococcus faecium (2.7%), Stenotrophomonas maltophilia (2.4%), Salmonella spp. (2.3%), and Streptococcus agalactiae (2.0%). The commonest pathogens apart from CoNS in age group 0-28 days, 29 days-2 months, 3-11 months, 1-5 years, and ≥ 5 years were Escherichia coli (17.2%), Escherichia coli (14.0%), Escherichia coli (7.9%), Streptococcus pneumoniae (10.7%) ,and Staphylococcus aureus (13.6%), respectively. The overall prevalence of extended-spectrum β-lactamases-producing Enterobacteriaceae, carbapenem-resistant Klebsiella pneumoniae, carbapenem-resistant Acinetobacter baumannii, and carbapenem-resistant Pseudomonas aeruginosa were 41.4, 28.4, 31.7, and 5.6%, respectively. The overall prevalence of methicillin-resistant Staphylococcus aureus, penicillin-resistant Streptococcus pneumoniae and vancomycin-resistant Enterococcus was 38.1, 28.3, and 0.7%, respectively. CONCLUSIONS: The major bacterial pathogens have differences in different age groups, ward types, and regions in Chinese children, and the commonest causing microorganism was the Escherichia coli, especially in neonates and infants. High prevalence of important resistant phenotypes is of a serious concern.
OBJECTIVES: This study aimed to investigate the microbiological profiles and antimicrobial resistance patterns of bloodstream pathogens in Chinese children. METHODS: This retrospective study was conducted at 13 tertiary hospitals in China during 2016-2018. The first bloodstream isolates of the same species from one pediatric patient < 18 years were included to this study for analysis. Antimicrobial susceptibility testing was determined based on minimum inhibitory concentrations or Kirby-Bauer disk diffusion methods according to the 2018 Clinical and Laboratory Standards Institute guidelines. RESULTS: Overall, 9345 nonduplicate bloodstream isolates were collected. Top 10 pathogens included Coagulase-negative staphylococcus (CoNS) (44.4%), Escherichia coli (10.2%), Klebsiella pneumoniae (5.9%), Staphylococcus aureus (5.0%), Streptococcus pneumoniae (4.9%), Pseudomonas aeruginosa(2.8%), Enterococcus faecium (2.7%), Stenotrophomonas maltophilia (2.4%), Salmonella spp. (2.3%), and Streptococcus agalactiae (2.0%). The commonest pathogens apart from CoNS in age group 0-28 days, 29 days-2 months, 3-11 months, 1-5 years, and ≥ 5 years were Escherichia coli (17.2%), Escherichia coli (14.0%), Escherichia coli (7.9%), Streptococcus pneumoniae (10.7%) ,and Staphylococcus aureus (13.6%), respectively. The overall prevalence of extended-spectrum β-lactamases-producing Enterobacteriaceae, carbapenem-resistant Klebsiella pneumoniae, carbapenem-resistant Acinetobacter baumannii, and carbapenem-resistant Pseudomonas aeruginosa were 41.4, 28.4, 31.7, and 5.6%, respectively. The overall prevalence of methicillin-resistant Staphylococcus aureus, penicillin-resistant Streptococcus pneumoniae and vancomycin-resistant Enterococcus was 38.1, 28.3, and 0.7%, respectively. CONCLUSIONS: The major bacterial pathogens have differences in different age groups, ward types, and regions in Chinese children, and the commonest causing microorganism was the Escherichia coli, especially in neonates and infants. High prevalence of important resistant phenotypes is of a serious concern.
Authors: Pei Yee Woh; May Pui Shan Yeung; William Bernard Goggins; Norman Lo; Kam Tak Wong; Viola Chow; Ka Yee Chau; Kitty Fung; Zigui Chen; Margaret Ip Journal: Microbiol Spectr Date: 2021-08-04