| Literature DB >> 33077975 |
Daniela Amann-Zalcenstein1,2, Luyi Tian1,3,4, Jaring Schreuder1, Sara Tomei1,3, Dawn S Lin1,3, Kirsten A Fairfax5,6,7, Jessica E Bolden5,6, Mark D McKenzie5,8, Andrew Jarratt5,6, Adrienne Hilton5,6, Jacob T Jackson1,5, Ladina Di Rago6, Matthew P McCormack9, Carolyn A de Graaf5,6, Olivia Stonehouse3,4, Samir Taoudi4,5, Warren S Alexander5,6, Stephen L Nutt1,5, Matthew E Ritchie4,5, Ashley P Ng5,6,10, Shalin H Naik11,12,13.
Abstract
A classical view of blood cell development is that multipotent hematopoietic stem and progenitor cells (HSPCs) become lineage-restricted at defined stages. Lin-c-Kit+Sca-1+Flt3+ cells, termed lymphoid-primed multipotent progenitors (LMPPs), have lost megakaryocyte and erythroid potential but are heterogeneous in their fate. Here, through single-cell RNA sequencing, we identify the expression of Dach1 and associated genes in this fraction as being coexpressed with myeloid/stem genes but inversely correlated with lymphoid genes. Through generation of Dach1-GFP reporter mice, we identify a transcriptionally and functionally unique Dach1-GFP- subpopulation within LMPPs with lymphoid potential with low to negligible classic myeloid potential. We term these 'lymphoid-primed progenitors' (LPPs). These findings define an early definitive branch point of lymphoid development in hematopoiesis and a means for prospective isolation of LPPs.Entities:
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Year: 2020 PMID： 33077975 DOI： 10.1038/s41590-020-0799-x
Source DB: PubMed Journal: Nat Immunol ISSN： 1529-2908 Impact factor: 25.606