Literature DB >> 3307775

Purification and characterization of recombinant human interleukin-1 beta produced in Escherichia coli.

Y Kikumoto, Y M Hong, T Nishida, S Nakai, Y Masui, Y Hirai.   

Abstract

Recombinant human interleukin-1 beta (rIL-1 beta) produced in Escherichia coli was purified to homogeneity by a combination of mass ion exchange column chromatography, ion exchange and gel filtration high performance liquid chromatography. The purified rIL-1 beta had a molecular weight of 18 kD on SDS-polyacrylamide gel electrophoresis and an isoelectric point of 6.9 on analytical isoelectric focusing. These values were almost same as those of natural interleukin-1 beta. The amino acid composition and amino acid sequence of the amino terminal region were consistent with those deduced from the cDNA sequence. In addition, the primary structure was confirmed by peptide mapping with lysyl-endopeptidase on reverse phase HPLC. Besides rIL-1 beta with amino terminal Ala, two molecular species, [Met0] rIL-1 beta and [desAla1] rIL-1 beta, were also obtained. Biological and physicochemical properties of the three species of rIL-1 beta were compared.

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Year:  1987        PMID： 3307775     DOI： 10.1016/s0006-291x(87)80123-7

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  14 in total

1.  The pH dependence of interleukin-1 beta effects on insulin secretion in HIT cells.

Authors:  H Shimizu; Y Uehara; N Sato; M Mori; Y Shimomura
Journal:  J Endocrinol Invest       Date:  1995-09       Impact factor: 4.256

2.  Isolation and bioactivities of three IL-1 beta N-terminal variants.

Authors:  K A Richard; A W Yem; M R Deibel; N D Staite
Journal:  Agents Actions       Date:  1989-06

3.  Survival of hemopoietic progenitors in the G0 period of the cell cycle does not require early hemopoietic regulators.

Authors:  A G Leary; Y Hirai; T Kishimoto; S C Clark; M Ogawa
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

4.  A simplified recipe for assigning amide NMR signals using combinatorial 14N amino acid inverse-labeling.

Authors:  Hidekazu Hiroaki; Yoshitaka Umetsu; Yo-ichi Nabeshima; Minako Hoshi; Daisuke Kohda
Journal:  J Struct Funct Genomics       Date:  2011-08-25

5.  Enhancement by galactosamine of lipopolysaccharide(LPS)-induced tumour necrosis factor production and lethality: its suppression by LPS pretreatment.

Authors:  Y Endo; M Shibazaki; K Yamaguchi; K Kai; S Sugawara; H Takada; H Kikuchi; K Kumagai
Journal:  Br J Pharmacol       Date:  1999-09       Impact factor: 8.739

6.  Participation of interleukin-1 and tumor necrosis factor in the responses of the sympathetic nervous system during lipopolysaccharide-induced fever.

Authors:  T Saigusa
Journal:  Pflugers Arch       Date:  1990-05       Impact factor: 3.657

7.  Characterization and mechanism of fever induction by interleukin-1 beta.

Authors:  M Hashimoto
Journal:  Pflugers Arch       Date:  1991-12       Impact factor: 3.657

8.  Preventive effects of interleukin 1 beta for ACNU-induced myelosuppression in malignant brain tumors: the experimental and preliminary clinical studies.

Authors:  S Tanaka; S Tabuchi; K Watanabe; H Takigawa; K Akatsuka; H Numata; Y Hokama; T Hori
Journal:  J Neurooncol       Date:  1992-10       Impact factor: 4.130

9.  Chemical modification of interleukin-1 beta: biochemical characterization of a carbodiimide-catalyzed intramolecular cross-linked protein.

Authors:  A W Yem; D M Guido; W R Mathews; N D Staite; K A Richard; M D Prairie; W C Krueger; D E Epps; M R Deibel
Journal:  J Protein Chem       Date:  1992-12

10.  Stability of interleukin 1 beta (IL-1 beta) in aqueous solution: analytical methods, kinetics, products, and solution formulation implications.

Authors:  L C Gu; E A Erdös; H S Chiang; T Calderwood; K Tsai; G C Visor; J Duffy; W C Hsu; L C Foster
Journal:  Pharm Res       Date:  1991-04       Impact factor: 4.200
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