| Literature DB >> 33074539 |
Emily Wiseman1, Lauren Bates2, David J Carroll3.
Abstract
To provide a better understanding of the composition of the egg cell membrane, we describe a method in which proteins and peptides that are either naturally released by the egg or cleaved by sperm proteases can be collected, analyzed, and identified. Such molecules are captured and isolated from the surrounding seawater via biotinylation, before being concentrated by an affinity interaction and subsequently analyzed by western blotting and mass spectrometry.Entities:
Keywords: Biotinylation; Blotting; Cell surface; Egg activation; Fertilization; Signal transduction
Mesh：
Substances：
Year: 2021 PMID： 33074539 DOI： 10.1007/978-1-0716-0974-3_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745