Literature DB >> 33074532

Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata.

Ryusaku Deguchi1, Takeshi Nakano2.   

Abstract

Pseudopotamilla occelata is a polychaete worm distributed widely in the northern part of the Pacific coast, having value as fishing bait as well as biological material for some basic research areas, including reproduction. Here we describe methods for handling the gametes and embryos of this worm, focusing on such topics as maintenance of adults, induction of oocyte maturation and fertilization, culture of embryos and larvae, microinjection into oocytes, and calcium (Ca2+) imaging.

Entities:  

Keywords:  Calcium; Embryo; Fertilization; Gametes; Microinjection; Oocyte maturation; Polychaete

Mesh：

Substances：

Year:  2021        PMID： 33074532     DOI： 10.1007/978-1-0716-0974-3_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

Review 1.  Measurement of intracellular calcium.

Authors:  A Takahashi; P Camacho; J D Lechleiter; B Herman
Journal:  Physiol Rev       Date:  1999-10       Impact factor: 37.312

2.  The evolution of annelids reveals two adaptive routes to the interstitial realm.

Authors:  Torsten Hugo Struck; Anja Golombek; Anne Weigert; Franziska Anni Franke; Wilfried Westheide; Günter Purschke; Christoph Bleidorn; Kenneth Michael Halanych
Journal:  Curr Biol       Date:  2015-07-23       Impact factor: 10.834

Review 3.  Diving into marine genomics with CRISPR/Cas9 systems.

Authors:  Tsuyoshi Momose; Jean-Paul Concordet
Journal:  Mar Genomics       Date:  2016-10-12       Impact factor: 1.710

Review 4.  Intracellular calcium signaling in the fertilized eggs of Annelida.

Authors:  Takeshi Nakano; Ryusaku Deguchi; Keiichiro Kyozuka
Journal:  Biochem Biophys Res Commun       Date:  2014-06-19       Impact factor: 3.575

5.  Illuminating the base of the annelid tree using transcriptomics.

Authors:  Anne Weigert; Conrad Helm; Matthias Meyer; Birgit Nickel; Detlev Arendt; Bernhard Hausdorf; Scott R Santos; Kenneth M Halanych; Günter Purschke; Christoph Bleidorn; Torsten H Struck
Journal:  Mol Biol Evol       Date:  2014-02-23       Impact factor: 16.240

6.  Soluble sperm extract specifically recapitulates the initial phase of the Ca2+ response in the fertilized oocyte of P. occelata following a G-protein/ PLCβ signaling pathway.

Authors:  Takeshi Nakano; Keiichiro Kyozuka
Journal:  Zygote       Date:  2014-10-16       Impact factor: 1.442

7.  Spatiotemporal characteristics and mechanisms of intracellular Ca(2+) increases at fertilization in eggs of jellyfish (Phylum Cnidaria, Class Hydrozoa).

Authors:  Ryusaku Deguchi; Eri Kondoh; Junko Itoh
Journal:  Dev Biol       Date:  2005-03-15       Impact factor: 3.582

Review 8.  Comparative biology of cAMP-induced germinal vesicle breakdown in marine invertebrate oocytes.

Authors:  Ryusaku Deguchi; Noriyo Takeda; Stephen A Stricker
Journal:  Mol Reprod Dev       Date:  2011-07-19       Impact factor: 2.609

9.  Ca2+-sensitive fluorescent dyes and intracellular Ca2+ imaging.

Authors:  Martin D Bootman; Katja Rietdorf; Tony Collins; Simon Walker; Michael Sanderson
Journal:  Cold Spring Harb Protoc       Date:  2013-02-01

10.  Novel two-step Ca2+ increase and its mechanisms and functions at fertilization in oocytes of the annelidan worm Pseudopotamilla occelata.

Authors:  Takeshi Nakano; Keiichiro Kyozuka; Ryusaku Deguchi
Journal:  Dev Growth Differ       Date:  2008-04-28       Impact factor: 2.053
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