Yunhui Jo1, Geon Oh2, Yongha Gi2, Heehun Sung2, Eun Bin Joo3, Suk Lee3, Myonggeun Yoon2. 1. Department of Bio-convergence Engineering, Korea University, Seoul, Korea. 2. Department of Bio-medical Engineering, Korea University, Seoul, Korea. 3. Department of Radiation Oncology, College of Medicine, Korea University, Seoul, Korea.
Abstract
PURPOSE: Tumor treating fields (TTF) therapy is a noninvasive method that uses alternating electric fields to treat various types of cancer. This study demonstrates the combined effect of TTF and radiotherapy (RT) in vitro on pancreatic cancer, which is known to be difficult to treat. MATERIALS AND METHODS: In CFPAC-I and HPAF-II pancreatic cancer cell lines, the combined in vitro effect of TTF and RT was evaluated by measuring cell counts, markers of apoptosis, and clonogenic cell survival. The synergy effects were verified using the Valeriote and Carpentier equations. RESULTS: TTF and RT inhibited cancer cell growth more effectively than did monotherapy with TTF or RT. The combined treatment also enhanced apoptosis more than monotherapy, as shown by assays for cleaved poly (ADP-ribose) polymerase (PARP) and annexin V. In addition, on the survival curve, this treatment method has been shown to work synergistically. CONCLUSION: These results suggest that combined treatment with TTF and RT may be a good alternative treatment for patients with pancreatic cancer.
PURPOSE: Tumor treating fields (TTF) therapy is a noninvasive method that uses alternating electric fields to treat various types of cancer. This study demonstrates the combined effect of TTF and radiotherapy (RT) in vitro on pancreatic cancer, which is known to be difficult to treat. MATERIALS AND METHODS: In CFPAC-I and HPAF-II pancreatic cancer cell lines, the combined in vitro effect of TTF and RT was evaluated by measuring cell counts, markers of apoptosis, and clonogenic cell survival. The synergy effects were verified using the Valeriote and Carpentier equations. RESULTS: TTF and RT inhibited cancer cell growth more effectively than did monotherapy with TTF or RT. The combined treatment also enhanced apoptosis more than monotherapy, as shown by assays for cleaved poly (ADP-ribose) polymerase (PARP) and annexin V. In addition, on the survival curve, this treatment method has been shown to work synergistically. CONCLUSION: These results suggest that combined treatment with TTF and RT may be a good alternative treatment for patients with pancreatic cancer.
Authors: Liping Bai; Tobias Pfeifer; Wolfgang Gross; Carolina De La Torre; Shuyang Zhao; Li Liu; Michael Schaefer; Ingrid Herr Journal: Front Oncol Date: 2021-11-03 Impact factor: 5.738