Jian-Hua Chen1,2, Edmund Cheung So1,2,3, Ping-Ching Wu4, Wen-Shuo Kuo5,6, Chia-Yuan Chang7, Jui-Chang Liu8. 1. Department of Anesthesia & Medicine Research, An Nan Hospital, China Medical University, Tainan 709, Taiwan Republic of China. 2. Department of Anesthesia, China Medical University, Taichung 404, Taiwan Republic of China. 3. Graduate Institute of Medical Sciences, Chang Jung Christian University, Tainan 711, Taiwan Republic of China. 4. Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan Republic of China. 5. School of Chemistry and Materials Science, Nanjing University of Information Science and Technology, Nanjing, Jiangsu 210044, People's Republic of China. 6. Allergy & Clinical Immunology Research Center, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan Republic of China. 7. Department of Mechanical Engineering, National Cheng Kung University, Tainan 701, Taiwan Republic of China. 8. Department of Biochemistry and Molecular Biology, National Cheng Kung University, Tainan 701, Taiwan Republic of China.
Abstract
BACKGROUND: Multidrug-resistant (MDR) bacterial strain is a serious medical problem. Methicillin-resistant Staphylococcus aureus (MRSA) is resistant to many antibiotics and is often associated with several diseases such as arthritis, osteomyelitis, and endocarditis. The development of an alternative treatment for eliminating MDR bacteria such as MRSA has attracted a considerable amount of research attention. Moreover, the development of a material for highly efficient generation of reactive oxygen species (ROS) involving two-photon photodynamic therapy (PDT) is currently desirable. MATERIALS AND METHODS: We present an example demonstrating that the use of water-soluble C60(OH)30 fullerenol with a 0.89 singlet oxygen quantum yield serving as a photosensitizer in PDT has the superior ability in effectively generating ROS. RESULTS: It has ultra-low energy (228.80 nJ pixel-1) and can perform 900 scans under two-photon excitation (TPE) in the near-infrared region (760 nm) to completely eliminate the MDR species. Furthermore, the favorable two-photon properties are absorption of approximately 760 nm in wavelength, absolute cross-section of approximately 1187.50 Göeppert-Mayer units, lifetime of 6.640 ns, ratio of radiative to nonradiative decay rates of approximately 0.053, and two-photon stability under TPE. CONCLUSION: This enabled water-soluble C60(OH)30 fullerenol to act as a promising two-photon photosensitizer proceeding with PDT to easily eliminate MDR species.
BACKGROUND: Multidrug-resistant (MDR) bacterial strain is a serious medical problem. Methicillin-resistant Staphylococcus aureus (MRSA) is resistant to many antibiotics and is often associated with several diseases such as arthritis, osteomyelitis, and endocarditis. The development of an alternative treatment for eliminating MDR bacteria such as MRSA has attracted a considerable amount of research attention. Moreover, the development of a material for highly efficient generation of reactive oxygen species (ROS) involving two-photon photodynamic therapy (PDT) is currently desirable. MATERIALS AND METHODS: We present an example demonstrating that the use of water-soluble C60(OH)30 fullerenol with a 0.89 singlet oxygen quantum yield serving as a photosensitizer in PDT has the superior ability in effectively generating ROS. RESULTS: It has ultra-low energy (228.80 nJ pixel-1) and can perform 900 scans under two-photon excitation (TPE) in the near-infrared region (760 nm) to completely eliminate the MDR species. Furthermore, the favorable two-photon properties are absorption of approximately 760 nm in wavelength, absolute cross-section of approximately 1187.50 Göeppert-Mayer units, lifetime of 6.640 ns, ratio of radiative to nonradiative decay rates of approximately 0.053, and two-photon stability under TPE. CONCLUSION: This enabled water-soluble C60(OH)30 fullerenol to act as a promising two-photon photosensitizer proceeding with PDT to easily eliminate MDR species.
Authors: Hannes C Schniepp; Je-Luen Li; Michael J McAllister; Hiroaki Sai; Margarita Herrera-Alonso; Douglas H Adamson; Robert K Prud'homme; Roberto Car; Dudley A Saville; Ilhan A Aksay Journal: J Phys Chem B Date: 2006-05-04 Impact factor: 2.991
Authors: Li Cao; Xin Wang; Mohammed J Meziani; Fushen Lu; Haifang Wang; Pengju G Luo; Yi Lin; Barbara A Harruff; L Monica Veca; Davoy Murray; Su-Yuan Xie; Ya-Ping Sun Journal: J Am Chem Soc Date: 2007-08-28 Impact factor: 15.419