Genomic Assemblies of Members of Burkholderia and Related Genera as a Resource for Natural Product Discovery.

The genomes of 450 members of Burkholderiaceae, isolated from clinical and environmental sources, were sequenced and assembled as a resource for genome mining. Genomic analysis of the collection has enabled the identification of multiple metabolites and their biosynthetic gene clusters, including the antibiotics gladiolin, icosalide A, enacyloxin, and cepacin A.

ANNOUNCEMENT

Burkholderia is a diverse genus of bacteria and a rich source of antimicrobial compounds (1). Modern natural product discovery is often guided by genome mining (2) and increasingly relies on large genome collections. Therefore, we have sequenced, assembled, and deposited into public databases a substantial collection of genomes of members of Burkholderia and related genera to promote natural product discovery.

Genomic DNA was extracted, using the automated Maxwell 16 instrument with the tissue extraction kit (Promega), from Burkholderia strains and strains of related genera that had been grown in tryptic soy broth overnight at 30°C or 37°C. HiSeq 2000 and HiSeq X Ten instruments, with the associated Illumina HiSeq kits for library preparation, generated 125-bp and 150-bp paired-end reads, respectively. Reads were trimmed using Trim Galore v0.4.2 (3), their quality was assessed using FastQC v0.10.1 (4), and overlapping reads were merged using FLASH v1.2.11 (5). The resulting reads were assembled into contigs using the assembler SPAdes v3.9.1 (6). Pilon v1.2125 (7) identified and corrected misassembled contigs, and contigs representing contaminating DNA were identified with Kraken v0.10.5-beta (8) using the MiniKraken database. In addition, four Burkholderia strains were sequenced with a Pacific Bioscience RS II instrument using genomic DNA prepared with the SMRTbell template preparation kit v2.0. The sequencing was performed in two runs with P4-C2 and P5-C3 chemistries, and the genomes were assembled with SMRT Analysis v2.3.0 as described previously (9). A quality assessment of the final genome assemblies was performed with QUAST v4.4 (10) (Table 1). Genomic identification was performed by average nucleotide identity (ANI) analyses with fastANI v1.2 (11) and pyani v0.2.9 (ANI using MUMmer [ANIm] to align input sequences) (12), using a ≥95% species delineation threshold (11). The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) v4.12 was used for genome annotation (13). Bioinformatics were supported by the Cloud Infrastructure for Microbial Bioinformatics (CLIMB) (14). Default software parameters were used except where otherwise noted.

TABLE 1

Strain information and genome assembly statistics for the Burkholderiaceae genome collection

Species	Strain	Isolation source	Geographic location	Read BioSample accession no.	Assembly BioSample accession no.	Assembly GenBank accession no.	Coverage (×) (technology)	Genome size (Mbp)	GC content (%)	N50 (bp)
Burkholderia ambifaria	BCC0118	Clinical, sputum	USA	ERS784989	ERS4267623	GCA_902829755	81 (Illumina)	7.50	66.82	273,879
Burkholderia ambifaria	BCC0191	Environmental, soil	USA	ERS784799	ERS4267624	GCA_902829465	82 (Illumina)	7.58	66.56	302,202
Burkholderia ambifaria	BCC0192	Environmental	USA	ERS785047	ERS4267625	GCA_902829785	73 (Illumina)	7.40	66.73	277,504
Burkholderia ambifaria	BCC0197	Environmental, Sesbania exaltata leaves		ERS785076	ERS4267626	GCA_902829415	71 (Illumina)	7.38	66.67	386,867
Burkholderia ambifaria	BCC0250	Clinical, sputum	Australia	ERS784819	ERS4267627	GCA_902829765	86 (Illumina)	7.36	66.84	396,147
Burkholderia ambifaria	BCC0267	Clinical, cystic fibrosis patient	Australia	ERS784835	ERS4267628	GCA_902829645	80 (Illumina)	7.36	66.76	784,052
Burkholderia ambifaria	BCC0284	Environmental, corn	USA	ERS1328916	ERS4267629	GCA_902829725	59 (Illumina)	7.47	66.68	423,851
Burkholderia ambifaria	BCC0316	Environmental, soil	USA	ERS784850	ERS4267630	GCA_902829685	83 (Illumina)	7.64	66.54	292,321
Burkholderia ambifaria	BCC0338	Environmental, corn roots	USA	ERS1371637	ERS4267631	GCA_902829825	90 (Illumina)	7.45	66.70	514,723
Burkholderia ambifaria	BCC0399	Environmental	USA	ERS785037	ERS4267632	GCA_902829775	77 (Illumina)	7.40	66.76	407,605
Burkholderia ambifaria	BCC0410	Environmental, maize	Italy	ERS784866	ERS4267633	GCA_902829705	93 (Illumina)	7.38	66.78	849,830
Burkholderia ambifaria	BCC0423	Environmental, maize	Italy	ERS1336067	ERS4267634	GCA_902829805	73 (Illumina)	7.47	66.76	382,759
Burkholderia ambifaria	BCC0477	Clinical, cystic fibrosis patient	USA	ERS784882	ERS4267635	GCA_902829695	99 (Illumina)	7.81	66.47	255,598
Burkholderia ambifaria	BCC0478	Clinical, cystic fibrosis patient	USA	ERS784897	ERS4267636	GCA_902829665	109 (Illumina)	7.24	66.82	601,161
Burkholderia ambifaria	BCC0480	Environmental, soil	USA	ERS784913	ERS4267637	GCA_902829735	113 (Illumina)	7.84	66.57	570,779
Burkholderia ambifaria	BCC1041	Environmental	Italy	ERS784930	ERS4267638	GCA_902829815	104 (Illumina)	7.51	66.70	469,567
Burkholderia ambifaria	BCC1048	Environmental	Italy	ERS784886	ERS4267639	GCA_902829675	70 (Illumina)	7.51	66.70	469,249
Burkholderia ambifaria	BCC1052	Environmental	Italy	ERS1371632	ERS4267640	GCA_902829435	72 (Illumina)	7.33	66.81	397,259
Burkholderia ambifaria	BCC1062	Environmental	Italy	ERS1328829	ERS4267641	GCA_902829655	65 (Illumina)	7.45	66.69	382,455
Burkholderia ambifaria	BCC1065	Environmental	Italy	ERS784959	ERS4267642	GCA_902829835	77 (Illumina)	7.31	66.79	605,534
Burkholderia ambifaria	BCC1066	Environmental	Italy	ERS784800	ERS4267643	GCA_902829905	87 (Illumina)	7.32	66.78	381,533
Burkholderia ambifaria	BCC1072	Environmental	Italy	ERS1328913	ERS4267644	GCA_902830005	66 (Illumina)	7.44	66.75	397,405
Burkholderia ambifaria	BCC1080	Environmental	Italy	ERS1371635	ERS4267645	GCA_902829995	84 (Illumina)	7.29	66.76	383,339
Burkholderia ambifaria	BCC1083	Environmental	Italy	ERS1328835	ERS4267646	GCA_902829965	63 (Illumina)	7.30	66.76	373,328
Burkholderia ambifaria	BCC1086	Environmental	Italy	ERS1328827	ERS4267647	GCA_902829915	70 (Illumina)	7.58	66.72	395,516
Burkholderia ambifaria	BCC1088	Environmental	Italy	ERS1371633	ERS4267648	GCA_902829875	87 (Illumina)	7.48	66.70	397,405
Burkholderia ambifaria	BCC1090	Environmental	Italy	ERS1328833	ERS4267649	GCA_902829975	68 (Illumina)	7.33	66.76	340,920
Burkholderia ambifaria	BCC1092	Environmental	Italy	ERS784808	ERS4267650	GCA_902829935	77 (Illumina)	7.38	66.78	849,686
Burkholderia ambifaria	BCC1093	Environmental	Italy	ERS784821	ERS4267651	GCA_902829945	81 (Illumina)	7.40	66.76	427,669
Burkholderia ambifaria	BCC1095	Environmental	Italy	ERS784837	ERS4267652	GCA_902829885	84 (Illumina)	7.40	66.76	407,577
Burkholderia ambifaria	BCC1098	Environmental	Italy	ERS784852	ERS4267653	GCA_902829895	83 (Illumina)	7.38	66.78	849,506
Burkholderia ambifaria	BCC1100	Environmental	Italy	ERS784868	ERS4267654	GCA_902830015	80 (Illumina)	7.40	66.76	407,581
Burkholderia ambifaria	BCC1103	Environmental	Italy	ERS784884	ERS4267655	GCA_902829985	86 (Illumina)	7.40	66.76	427,387
Burkholderia ambifaria	BCC1105	Environmental	Italy	ERS1371636	ERS4267656	GCA_902829845	97 (Illumina)	6.30	66.77	1,592,784
Burkholderia ambifaria	BCC1107	Environmental	Italy	ERS784899	ERS4267657	GCA_902829855	88 (Illumina)	7.40	66.76	374,059
Burkholderia ambifaria	BCC1212	Environmental, rhizosphere	USA	ERS1371638	ERS4267658	GCA_902829955	82 (Illumina)	7.61	66.44	358,611
Burkholderia ambifaria	BCC1213	Environmental	USA	ERS1328957	ERS4267659	GCA_902829925	65 (Illumina)	7.47	66.70	425,516
Burkholderia ambifaria	BCC1214	Environmental	USA	ERS784915	ERS4267660	GCA_902829595	102 (Illumina)	7.61	66.40	270,842
Burkholderia ambifaria	BCC1216	Environmental	USA	ERS1328918	ERS4267661	GCA_902829865	67 (Illumina)	7.46	66.69	234,327
Burkholderia ambifaria	BCC1218	Environmental	USA	ERS784932	ERS4267662	GCA_902830025	88 (Illumina)	7.46	66.67	348,377
Burkholderia ambifaria	BCC1220	Environmental	USA	ERS1328837	ERS4267663	GCA_902830035	65 (Illumina)	7.64	66.39	393,412
Burkholderia ambifaria	BCC1223	Environmental	USA	ERS784947	ERS4267664	GCA_902830105	104 (Illumina)	7.61	66.40	267,383
Burkholderia ambifaria	BCC1224	Environmental, maize	USA	ERS1328836	ERS4267665	GCA_902829395	51 (Illumina)	8.46	63.24	317,020
Burkholderia ambifaria	BCC1228	Environmental, maize	USA	ERS1328832	ERS4267666	GCA_902830065	62 (Illumina)	7.38	66.73	558,864
Burkholderia ambifaria	BCC1229	Environmental, maize	USA	ERS1371639	ERS4267667	GCA_902830045	65 (Illumina)	7.51	66.70	298,531
Burkholderia ambifaria	BCC1233	Environmental, maize	USA	ERS1328917	ERS4267668	GCA_902830075	57 (Illumina)	7.96	66.24	390,415
Burkholderia ambifaria	BCC1236	Environmental, maize	USA	ERS1328839	ERS4267669	GCA_902830095	64 (Illumina)	7.63	66.60	358,767
Burkholderia ambifaria	BCC1237	Environmental, maize	USA	ERS1371640	ERS4267670	GCA_902830055	66 (Illumina)	7.34	66.84	420,767
Burkholderia ambifaria	BCC1240	Environmental, maize	USA	ERS1328914	ERS4267671	GCA_902830085	57 (Illumina)	7.40	66.77	503,900
Burkholderia ambifaria	BCC1241	Environmental, maize	USA	ERS784961	ERS4267672	GCA_902830175	78 (Illumina)	7.68	66.49	288,780
Burkholderia ambifaria	BCC1246	Environmental, maize	USA	ERS1328834	ERS4267673	GCA_902830205	69 (Illumina)	7.48	66.64	450,462
Burkholderia ambifaria	BCC1248	Environmental, maize	USA	ERS784801	ERS4267674	GCA_902830135	87 (Illumina)	8.03	66.48	241,997
Burkholderia ambifaria	BCC1249	Environmental, maize	USA	ERS1371634	ERS4267675	GCA_902830255	97 (Illumina)	7.58	66.75	447,443
Burkholderia ambifaria	BCC1252	Environmental, maize	USA	ERS784809	ERS4267676	GCA_902830125	83 (Illumina)	7.42	66.71	254,705
Burkholderia ambifaria	BCC1256	Environmental, maize	USA	ERS784823	ERS4267677	GCA_902830165	86 (Illumina)	7.54	66.60	281,063
Burkholderia ambifaria	BCC1258	Environmental, maize	USA	ERS1371641	ERS4267678	GCA_902829475	81 (Illumina)	7.60	66.61	272,850
Burkholderia ambifaria	BCC1259	Environmental, maize	USA	ERS784838	ERS4267679	GCA_902830225	78 (Illumina)	7.63	66.39	431,727
Burkholderia ambifaria	BCC1265	Environmental	USA	ERS784854	ERS4267680	GCA_902830115	79 (Illumina)	7.62	66.44	335,885
Burkholderia ambifaria	BCC1270	Environmental, maize	USA	ERS784870	ERS4267681	GCA_902830155	73 (Illumina)	7.60	66.61	315,858
Burkholderia multivorans	BCC1599	Environmental		ERS1371642	ERS4267682	GCA_902833025	87 (Illumina)	6.74	67.15	248,547
Burkholderia sp.	BCC1630	Environmental	Malaysia	ERS1328847	ERS4267683	GCA_902833165	75 (Illumina)	7.62	66.03	201,973
Burkholderia sp.	BCC1638	Environmental	Malaysia	ERS1328851	ERS4267684	GCA_902833195	89 (Illumina)	6.96	66.61	206,632
Burkholderia anthina	BCC0036	Clinical, cystic fibrosis patient	UK	ERS785067	ERS4267685	GCA_902830145	69 (Illumina)	7.72	66.49	216,836
Burkholderia anthina	BCC0403	Environmental, river	USA	ERS784934	ERS4267686	GCA_902830325	76 (Illumina)	8.15	66.41	375,294
Burkholderia anthina	BCC0485	Clinical, cystic fibrosis patient	USA	ERS1328919	ERS4267687	GCA_902829375	49 (Illumina)	9.30	61.27	152,611
Burkholderia anthina	BCC0518	Environmental, soil	UK	ERS1328922	ERS4267688	GCA_902830485	66 (Illumina)	8.28	66.46	220,529
Burkholderia anthina	BCC0634	Environmental, soil rhizosphere	USA	ERS1328841	ERS4267689	GCA_902830375	87 (Illumina)	7.94	65.74	168,505
Burkholderia anthina	BCC0635	Environmental, rhizosphere		ERS1333586	ERS4267690	GCA_902830445	89 (Illumina)	7.76	66.03	194,880
Burkholderia anthina	BCC0636	Environmental, Carludovica palmata rhizosphere	UK	ERS1328842	ERS4267691	GCA_902830345	95 (Illumina)	7.76	66.03	195,048
Burkholderia anthina	BCC0637	Environmental, soil		ERS1328920	ERS4267692	GCA_902830315	101 (Illumina)	7.30	66.36	205,801
Burkholderia anthina	BCC0638	Environmental, soil		ERS1328843	ERS4267693	GCA_902829625	102 (Illumina)	7.69	66.33	147,404
Burkholderia anthina	LMG 20980	Environmental, soil rhizosphere	USA	ERS1371643	ERS4267694	GCA_902830395	71 (Illumina)	7.63	66.74	336,735
Burkholderia anthina	BCC1349	Clinical		ERS1328844	ERS4267695	GCA_902830335	76 (Illumina)	8.61	66.32	444,914
Burkholderia stagnalis	BCC1350	Environmental		ERS784963	ERS4267696	GCA_902833065	83 (Illumina)	8.37	67.13	251,925
Burkholderia anthina	BCC1351	Clinical		ERS1328921	ERS4267697	GCA_902829335	94 (Illumina)	7.48	66.21	196,378
Burkholderia anthina	BCC1358	Clinical		ERS1371644	ERS4267698	GCA_902830365	68 (Illumina)	9.16	66.08	173,669
Burkholderia anthina	BCC1363	Environmental		ERS1336069	ERS4267699	GCA_902830415	85 (Illumina)	7.28	66.32	170,879
Burkholderia arboris	BCC0049	Clinical, non-cystic fibrosis patient	France	ERS785032	ERS4267700	GCA_902830425	64 (Illumina)	8.28	66.77	418,964
Burkholderia cenocepacia	J2315	Clinical, respiratory sample	UK	ERS784840	ERS4267701	GCA_902830575	82 (Illumina)	7.91	66.99	221,803
Burkholderia cenocepacia	BCC0016	Clinical, sputum	Canada	ERS784856	ERS4267702	GCA_902830355	81 (Illumina)	7.89	67.01	175,882
Burkholderia cenocepacia	BCC0017	Clinical, sputum	Canada	ERS785082	ERS4267703	GCA_902830435	79 (Illumina)	8.10	67.17	243,601
Burkholderia cepacia	BCC0018	Clinical, cystic fibrosis patient	UK	ERS785027	ERS4267704	GCA_902830685	59 (Illumina)	8.54	66.76	891,303
Burkholderia cenocepacia	BCC0020	Clinical, cystic fibrosis patient	Australia	ERS784871	ERS4267705	GCA_902830475	88 (Illumina)	7.30	66.88	536,526
Burkholderia cenocepacia	BCC0048	Clinical, non-cystic fibrosis patient	France	ERS784998	ERS4267706	GCA_902830385	64 (Illumina)	8.32	66.91	293,098
Burkholderia sp.	BCC0097	Clinical, respiratory sample	Canada	ERS784888	ERS4267707	GCA_902832985	65 (Illumina)	7.94	66.71	613,508
Burkholderia cenocepacia	BCC0107	Clinical, sputum	Canada	ERS785010	ERS4267708	GCA_902830455	68 (Illumina)	8.19	67.10	293,110
Burkholderia cenocepacia	BCC0127	Clinical, sputum	USA	ERS785018	ERS4267709	GCA_902830405	71 (Illumina)	7.73	66.79	420,157
Burkholderia contaminans	BCC0132	Clinical, sputum	Canada	ERS785057	ERS4267710	GCA_902830775	64 (Illumina)	8.46	66.61	151,177
Burkholderia cenocepacia	BCC0138	Clinical	Canada	ERS785048	ERS4267711	GCA_902830465	67 (Illumina)	7.99	67.24	228,664
Burkholderia cenocepacia	BCC0160	Clinical	USA	ERS784903	ERS4267712	GCA_902830505	81 (Illumina)	6.98	66.93	421,783
Burkholderia cenocepacia	BCC0165	Clinical, cystic fibrosis patient	Belgium	ERS785066	ERS4267713	GCA_902830495	73 (Illumina)	7.52	66.96	1,954,875
Burkholderia cenocepacia	BCC0199	Environmental	USA	ERS785064	ERS4267714	GCA_902830545	72 (Illumina)	7.97	66.66	275,726
Burkholderia cenocepacia	BCC0202	Environmental	USA	ERS784992	ERS4267715	GCA_902830535	84 (Illumina)	7.61	66.87	596,170
Burkholderia cenocepacia	BCC0313	Clinical		ERS784920	ERS4267716	GCA_902830515	77 (Illumina)	8.62	65.53	301,741
Burkholderia contaminans	BCC0320	Environmental, radish	Mexico	ERS784936	ERS4267717	GCA_902830835	84 (Illumina)	8.33	66.35	606,600
Burkholderia cenocepacia	BCC0451	Environmental, maize		ERS784950	ERS4267718	GCA_902830565	91 (Illumina)	7.52	66.92	1,207,947
Burkholderia sp.	BCC0506	Clinical, cystic fibrosis patient	Italy	ERS784858	ERS4267719	GCA_902833145	90 (Illumina)	7.29	67.22	506,111
Burkholderia sp.	BCC0801	Clinical, cystic fibrosis patient	Argentina	ERS784873	ERS4267720	GCA_902833175	88 (Illumina)	7.50	67.06	298,805
Burkholderia cenocepacia	BCC0999	Environmental		ERS784803	ERS4267721	GCA_902830585	96 (Illumina)	7.62	66.81	225,632
Burkholderia cenocepacia	BCC1028	Environmental		ERS784811	ERS4267722	GCA_902830605	91 (Illumina)	7.59	66.87	470,293
Burkholderia sp.	BCC1208	Environmental, lupine shoot endophyte	Australia	ERS784827	ERS4267723	GCA_902833095	91 (Illumina)	7.38	66.96	490,408
Burkholderia cenocepacia	BCC1266	Environmental		ERS785055	ERS4267724	GCA_902830595	65 (Illumina)	7.92	66.65	259,121
Burkholderia cenocepacia	BCC1322	Environmental/industrial		ERS784842	ERS4267725	GCA_902829445	82 (Illumina)	7.94	66.75	571,121
Burkholderia cenocepacia	BCC1556	Environmental/industrial	UK	ERS785001	ERS4267726	GCA_902830525	78 (Illumina)	7.21	67.13	1,136,705
Burkholderia cepacia	BCC1631	Environmental		ERS1328961	ERS4267727	GCA_902830715	71 (Illumina)	8.72	66.64	276,956
Burkholderia cepacia	BCC1632	Environmental		ERS1328848	ERS4267728	GCA_902830785	83 (Illumina)	8.83	66.59	273,961
Burkholderia cepacia	BCC1634	Environmental		ERS1328849	ERS4267729	GCA_902830765	90 (Illumina)	7.59	66.62	403,579
Burkholderia cepacia	BCC1636	Environmental		ERS1328850	ERS4267730	GCA_902829425	88 (Illumina)	8.48	66.70	266,835
Burkholderia cepacia	BCC1641	Environmental	Malaysia	ERS1328925	ERS4267731	GCA_902830805	80 (Illumina)	8.72	66.64	256,085
Burkholderia sp.	BCC1644	Environmental		ERS1371646	ERS4267732	GCA_902833225	71 (Illumina)	8.25	66.71	390,260
Burkholderia cenocepacia	BCC1718	Clinical		ERS785000	ERS4267733	GCA_902830555	70 (Illumina)	7.70	66.81	543,487
Burkholderia cepacia	BCC0001	Environmental, onion		ERS784996	ERS4267734	GCA_902830625	67 (Illumina)	8.51	66.65	467,216
Burkholderia cepacia	BCC0002	Environmental, forest soil	Trinidad and Tobago	ERS784890	ERS4267735	GCA_902830635	68 (Illumina)	8.54	66.76	891,307
Burkholderia cepacia	BCC0003	Clinical, cystic fibrosis patient	Australia	ERS784995	ERS4267736	GCA_902830665	68 (Illumina)	8.51	66.29	289,400
Burkholderia cepacia	BCC0196	Environmental, grass		ERS784922	ERS4267737	GCA_902830615	75 (Illumina)	8.53	66.58	453,915
Burkholderia ambifaria	BCC0200	Environmental		ERS785045	ERS4267738	GCA_902829565	76 (Illumina)	7.63	66.55	331,719
Burkholderia cepacia	BCC0233	Environmental		ERS785054	ERS4267739	GCA_902830645	66 (Illumina)	8.82	66.50	775,678
Burkholderia cepacia	BCC0256	Clinical, sputum	New Zealand	ERS784905	ERS4267740	GCA_902830655	59 (Illumina)	9.19	66.32	987,550
Burkholderia cepacia	BCC0367	Clinical		ERS784937	ERS4267741	GCA_902830675	86 (Illumina)	8.68	66.51	796,538
Burkholderia cepacia	BCC0970	Environmental		ERS784952	ERS4267742	GCA_902830695	77 (Illumina)	8.37	66.76	428,681
Burkholderia cepacia	BCC0974	Environmental		ERS784967	ERS4267743	GCA_902830705	86 (Illumina)	8.59	66.74	450,214
Burkholderia cepacia	BCC0977	Environmental		ERS784804	ERS4267744	GCA_902830755	106 (Illumina)	6.93	67.06	573,127
Burkholderia cepacia	BCC1058	Environmental		ERS784813	ERS4267745	GCA_902830745	81 (Illumina)	8.37	66.76	505,601
Burkholderia cepacia	BCC1273			ERS784829	ERS4267746	GCA_902830735	80 (Illumina)	8.51	66.80	984,555
Burkholderia sp.	BCC1640	Environmental	Malaysia	ERS1328852	ERS4267747	GCA_902833125	92 (Illumina)	7.20	66.40	186,730
Burkholderia sp.	BCC0044	Clinical, cystic fibrosis patient	UK	ERS785043	ERS4267748	GCA_902832955	70 (Illumina)	8.04	66.82	1,287,212
Burkholderia sp.	BCC0322	Clinical, cystic fibrosis patient	UK	ERS784908	ERS4267749	GCA_902833045	71 (Illumina)	7.75	66.27	527,417
Burkholderia sp.	BCC0397	Clinical		ERS784940	ERS4267750	GCA_902832865	71 (Illumina)	8.16	66.81	442,581
Burkholderia sp.	BCC0398	Environmental		ERS785051	ERS4267751	GCA_902833245	65 (Illumina)	8.01	66.54	387,212
Burkholderia sp.	BCC0405	Environmental		ERS784923	ERS4267752	GCA_902833075	71 (Illumina)	7.98	66.08	309,371
Burkholderia sp.	BCC0419	Environmental, maize		ERS784958	ERS4267753	GCA_902830285	66 (Illumina)	8.18	66.71	1,058,720
Burkholderia sp.	BCC1047	Environmental		ERS784973	ERS4267754	GCA_902833155	83 (Illumina)	7.79	66.79	153,691
Burkholderia pseudomultivorans	BCC1191	Clinical		ERS785049	ERS4267755	GCA_902832925	73 (Illumina)	7.55	67.02	243,992
Burkholderia contaminans	BCC0123	Clinical, sputum	USA	ERS785009	ERS4267756	GCA_902830725	60 (Illumina)	8.77	66.35	548,891
Burkholderia contaminans	BCC0254	Clinical, sputum	Australia	ERS784845	ERS4267757	GCA_902830865	79 (Illumina)	8.35	66.00	564,071
Burkholderia contaminans	BCC0339	Clinical		ERS784860	ERS4267758	GCA_902830845	76 (Illumina)	8.29	66.39	1,070,961
Burkholderia contaminans	BCC1315	Environmental/industrial		ERS784875	ERS4267759	GCA_902830875	75 (Illumina)	8.02	66.62	652,822
Burkholderia diffusa	BCC0106	Clinical, cystic fibrosis patient throat	Canada	ERS785072	ERS4267760	GCA_902830825	72 (Illumina)	7.80	65.92	283,477
Burkholderia diffusa	BCC0109	Clinical, respiratory sample	Canada	ERS785028	ERS4267761	GCA_902830855	76 (Illumina)	7.23	66.15	216,537
Burkholderia diffusa	LMG 29043	Environmental, soil	USA	ERS4267762	ERS4267762	GCA_902830815	180 (Illumina)	7.15	66.50	236,915
Burkholderia dolosa	BCC0306	Clinical	USA	ERS785034	ERS4267763	GCA_902829575	75 (Illumina)	6.50	67.06	519,080
Burkholderia gladioli	BCC0238	Clinical, sputum	USA	ERS333128	ERS4267764	GCA_902830885	63 (Illumina)	8.55	68.00	479,899
Burkholderia gladioli	BCC0252	Clinical, sputum	Australia	ERS784955	ERS4267765	GCA_902830905	89 (Illumina)	8.22	68.09	289,654
Burkholderia gladioli	BCC0507	Clinical, cystic fibrosis patient	Italy	ERS784970	ERS4267766	GCA_902830895	92 (Illumina)	8.36	68.13	335,266
Burkholderia gladioli	BCC0771	Environmental, Gladiolus sp.	USA	ERS784806	ERS4267767	GCA_902830915	80 (Illumina)	8.80	67.72	967,784
Burkholderia gladioli	BCC1317	Environmental/industrial	UK	ERS785017	ERS4267768	GCA_902830925	59 (Illumina)	8.77	67.93	552,562
Burkholderia gladioli	BCC1619	Clinical	USA	ERS784817	ERS4267769	GCA_902830945	89 (Illumina)	7.88	68.13	868,935
Burkholderia gladioli	BCC1620	Clinical	USA	ERS784832	ERS4267770	GCA_902830985	80 (Illumina)	8.19	68.09	416,357
Burkholderia gladioli	BCC1621	Clinical	USA	ERS784849	ERS4267771	GCA_902830995	75 (Illumina)	8.48	67.95	434,143
Burkholderia gladioli	BCC1622	Clinical	USA	ERS784864	ERS4267772	GCA_902830955	79 (Illumina)	8.41	68.05	615,150
Burkholderia gladioli	BCC1623	Clinical	USA	ERS784879	ERS4267773	GCA_902830935	75 (Illumina)	8.44	67.87	365,793
Burkholderia gladioli	BCC1624	Clinical	USA	ERS784895	ERS4267774	GCA_902830975	81 (Illumina)	7.88	68.14	430,243
Burkholderia gladioli	BCC1645	Environmental, Allium cepa bulb	Australia	ERS784911	ERS4267775	GCA_902830965	73 (Illumina)	8.61	67.87	383,325
Burkholderia gladioli	BCC1646	Environmental, Allium cepa	India	ERS784928	ERS4267776	GCA_902831005	78 (Illumina)	8.39	67.96	286,552
Burkholderia gladioli	BCC1647	Environmental, Gladiolus sp.	USA	ERS784943	ERS4267777	GCA_902831075	73 (Illumina)	8.88	67.70	561,180
Burkholderia gladioli	BCC1648	Environmental	USA	ERS784957	ERS4267778	GCA_902831035	79 (Illumina)	8.62	67.87	310,631
Burkholderia gladioli	BCC1649	Environmental, Gladiolus sp.	Zimbabwe	ERS784812	ERS4267779	GCA_902831025	67 (Illumina)	8.25	68.30	554,529
Burkholderia gladioli	BCC1650	Environmental, poisoned bongkrek	Indonesia	ERS784828	ERS4267780	GCA_902831015	67 (Illumina)	8.10	68.24	708,245
Burkholderia gladioli	BCC1651	Clinical	USA	ERS784844	ERS4267781	GCA_902829385	68 (Illumina)	7.94	68.29	595,470
Burkholderia gladioli	BCC1661	Clinical	USA	ERS784861	ERS4267782	GCA_902831055	63 (Illumina)	8.33	68.06	632,785
Burkholderia gladioli	BCC1662	Clinical	USA	ERS784878	ERS4267783	GCA_902831065	65 (Illumina)	8.51	68.05	410,006
Burkholderia gladioli	BCC1663	Clinical	USA	ERS784894	ERS4267784	GCA_902831085	82 (Illumina)	8.51	68.05	410,015
Burkholderia gladioli	BCC1664	Clinical	USA	ERS784910	ERS4267785	GCA_902831045	62 (Illumina)	8.51	68.06	378,407
Burkholderia gladioli	BCC1665	Clinical	USA	ERS784925	ERS4267786	GCA_902831105	72 (Illumina)	8.33	68.06	601,921
Burkholderia gladioli	BCC1666	Clinical	USA	ERS784942	ERS4267787	GCA_902831115	70 (Illumina)	8.51	68.05	408,038
Burkholderia gladioli	BCC1667	Clinical	UK	ERS784960	ERS4267788	GCA_902831095	71 (Illumina)	8.49	67.84	427,447
Burkholderia gladioli	BCC1668	Clinical	UK	ERS784974	ERS4267789	GCA_902831165	75 (Illumina)	8.46	67.97	603,154
Burkholderia gladioli	BCC1669	Clinical	UK	ERS784982	ERS4267790	GCA_902831175	78 (Illumina)	8.18	68.14	485,051
Burkholderia gladioli	BCC1670	Clinical	UK	ERS784814	ERS4267791	GCA_902829325	68 (Illumina)	8.29	68.03	309,426
Burkholderia gladioli	BCC1671	Clinical	UK	ERS784830	ERS4267792	GCA_902831225	62 (Illumina)	8.36	68.09	369,886
Burkholderia gladioli	BCC1672	Clinical	UK	ERS784846	ERS4267793	GCA_902831265	63 (Illumina)	8.28	68.03	287,015
Burkholderia gladioli	BCC1674	Clinical	UK	ERS784994	ERS4267794	GCA_902831195	68 (Illumina)	8.44	67.87	356,821
Burkholderia gladioli	BCC1675	Clinical	USA	ERS785062	ERS4267795	GCA_902831215	70 (Illumina)	8.09	68.09	936,495
Burkholderia gladioli	BCC1676	Clinical	USA	ERS784863	ERS4267796	GCA_902831315	70 (Illumina)	7.99	68.26	467,101
Burkholderia gladioli	BCC1677	Clinical	USA	ERS785071	ERS4267797	GCA_902831155	75 (Illumina)	8.11	68.25	632,433
Burkholderia gladioli	BCC1678	Clinical	USA	ERS784880	ERS4267798	GCA_902831285	71 (Illumina)	7.83	68.04	235,084
Burkholderia gladioli	BCC1679	Clinical	USA	ERS784896	ERS4267799	GCA_902831135	73 (Illumina)	8.12	68.09	272,665
Burkholderia gladioli	BCC1680	Clinical	USA	ERS784912	ERS4267800	GCA_902831205	68 (Illumina)	7.97	68.00	411,881
Burkholderia gladioli	BCC1681	Clinical	USA	ERS784927	ERS4267801	GCA_902831305	79 (Illumina)	7.33	68.09	382,283
Burkholderia gladioli	BCC1682	Clinical	USA	ERS784944	ERS4267802	GCA_902829635	82 (Illumina)	7.95	68.03	428,648
Burkholderia gladioli	BCC1683	Clinical	USA	ERS784962	ERS4267803	GCA_902831235	64 (Illumina)	8.31	68.17	886,986
Burkholderia gladioli	BCC1684	Clinical	USA	ERS784975	ERS4267804	GCA_902831185	76 (Illumina)	8.39	67.96	576,064
Burkholderia gladioli	BCC1685	Clinical	USA	ERS784983	ERS4267805	GCA_902829495	81 (Illumina)	7.95	68.03	314,381
Burkholderia gladioli	BCC1686	Clinical	USA	ERS784816	ERS4267806	GCA_902831255	88 (Illumina)	8.34	68.06	728,975
Burkholderia gladioli	BCC1687	Clinical	USA	ERS784833	ERS4267807	GCA_902831145	65 (Illumina)	8.19	68.21	336,464
Burkholderia gladioli	BCC1688	Clinical	USA	ERS784848	ERS4267808	GCA_902831125	67 (Illumina)	8.46	68.14	319,266
Burkholderia gladioli	BCC1689	Clinical	USA	ERS784865	ERS4267809	GCA_902831275	71 (Illumina)	8.26	67.94	353,209
Burkholderia gladioli	BCC1690	Clinical	USA	ERS784881	ERS4267810	GCA_902831295	71 (Illumina)	8.48	67.94	433,041
Burkholderia gladioli	BCC1691	Clinical	USA	ERS784898	ERS4267811	GCA_902831245	78 (Illumina)	8.43	68.04	1,964,774
Burkholderia gladioli	BCC1692	Clinical	USA	ERS784914	ERS4267812	GCA_902831375	65 (Illumina)	8.39	67.98	810,454
Burkholderia gladioli	BCC1693	Clinical	USA	ERS784929	ERS4267813	GCA_902831395	79 (Illumina)	8.22	68.10	668,608
Burkholderia gladioli	BCC1694	Clinical	USA	ERS784946	ERS4267814	GCA_902831415	69 (Illumina)	8.48	67.90	688,705
Burkholderia gladioli	BCC1695	Clinical	USA	ERS784964	ERS4267815	GCA_902831335	78 (Illumina)	7.91	68.06	538,303
Burkholderia gladioli	BCC1696	Clinical	USA	ERS784976	ERS4267816	GCA_902831405	84 (Illumina)	8.28	68.19	429,819
Burkholderia gladioli	BCC1697	Clinical	USA	ERS784984	ERS4267817	GCA_902831465	78 (Illumina)	8.09	68.02	652,756
Burkholderia gladioli	BCC1698	Clinical	USA	ERS784818	ERS4267818	GCA_902831425	77 (Illumina)	8.50	68.10	434,629
Burkholderia gladioli	BCC1699	Clinical	USA	ERS784834	ERS4267819	GCA_902831435	66 (Illumina)	8.35	67.87	354,820
Burkholderia gladioli	BCC1700	Clinical	USA	ERS784851	ERS4267820	GCA_902831515	67 (Illumina)	8.49	68.08	1,416,119
Burkholderia gladioli	BCC1701	Clinical	USA	ERS784867	ERS4267821	GCA_902829355	67 (Illumina)	8.15	67.98	280,973
Burkholderia gladioli	BCC1703	Clinical	USA	ERS785056	ERS4267822	GCA_902831355	64 (Illumina)	8.29	68.22	599,795
Caballeronia sp.	BCC1704	Clinical	USA	ERS784900	ERS4267823	GCA_902833455	87 (Illumina)	5.98	63.70	1,316,445
Burkholderia gladioli	BCC1705	Clinical	USA	ERS784916	ERS4267824	GCA_902831345	79 (Illumina)	8.15	68.17	551,683
Caballeronia zhejiangensis	BCC1706	Clinical	USA	ERS784931	ERS4267825	GCA_902833575	80 (Illumina)	7.02	63.19	368,929
Burkholderia gladioli	BCC1707	Clinical	USA	ERS784948	ERS4267826	GCA_902831505	81 (Illumina)	8.01	68.22	534,508
Burkholderia gladioli	BCC1708	Clinical	USA	ERS784966	ERS4267827	GCA_902831455	80 (Illumina)	8.34	68.03	945,197
Burkholderia gladioli	BCC1709	Clinical	USA	ERS785035	ERS4267828	GCA_902831485	62 (Illumina)	8.47	68.12	414,686
Burkholderia gladioli	BCC1710	Clinical	USA	ERS785039	ERS4267829	GCA_902831495	66 (Illumina)	8.38	68.02	615,977
Burkholderia gladioli	BCC1711	Clinical	USA	ERS785006	ERS4267830	GCA_902831365	71 (Illumina)	8.35	68.06	335,836
Burkholderia gladioli	BCC1712	Clinical	USA	ERS785025	ERS4267831	GCA_902831475	61 (Illumina)	8.21	68.08	388,388
Burkholderia gladioli	BCC1713	Clinical	USA	ERS785074	ERS4267832	GCA_902831445	66 (Illumina)	8.47	68.05	147,146
Burkholderia gladioli	BCC1714	Clinical	USA	ERS785080	ERS4267833	GCA_902831385	71 (Illumina)	8.50	68.08	361,363
Burkholderia gladioli	BCC1715	Clinical	USA	ERS784977	ERS4267834	GCA_902831325	82 (Illumina)	7.97	68.14	954,999
Burkholderia gladioli	BCC1716	Clinical	USA	ERS785052	ERS4267835	GCA_902831605	66 (Illumina)	8.50	67.87	385,075
Burkholderia gladioli	BCC1717	Clinical	USA	ERS785014	ERS4267836	GCA_902831675	58 (Illumina)	8.43	67.93	430,020
Burkholderia gladioli	BCC1719	Clinical	USA	ERS785042	ERS4267837	GCA_902831715	62 (Illumina)	7.99	68.17	429,154
Burkholderia gladioli	BCC1720	Clinical	USA	ERS784985	ERS4267838	GCA_902831695	83 (Illumina)	7.91	68.29	770,588
Burkholderia gladioli	BCC1721	Clinical	USA	ERS784820	ERS4267839	GCA_902831535	87 (Illumina)	8.22	68.23	1,289,329
Burkholderia gladioli	BCC1722	Clinical	USA	ERS784836	ERS4267840	GCA_902831595	71 (Illumina)	8.00	68.18	346,974
Burkholderia gladioli	BCC1723	Clinical	USA	ERS784853	ERS4267841	GCA_902831645	69 (Illumina)	8.19	68.25	1,570,053
Burkholderia gladioli	BCC1724	Clinical	USA	ERS785060	ERS4267842	GCA_902831545	60 (Illumina)	8.52	68.03	505,438
Burkholderia gladioli	BCC1725	Clinical	USA	ERS784869	ERS4267843	GCA_902831555	62 (Illumina)	8.57	68.03	521,790
Burkholderia gladioli	BCC1726	Clinical	USA	ERS784885	ERS4267844	GCA_902831525	71 (Illumina)	8.13	68.29	848,284
Burkholderia gladioli	BCC1727	Clinical	USA	ERS785068	ERS4267845	GCA_902829345	67 (Illumina)	8.16	68.29	921,754
Burkholderia gladioli	BCC1728	Clinical	USA	ERS785003	ERS4267846	GCA_902831685	74 (Illumina)	8.13	68.29	391,569
Burkholderia gladioli	BCC1729	Clinical	USA	ERS784902	ERS4267847	GCA_902831705	69 (Illumina)	8.38	67.89	662,719
Burkholderia gladioli	BCC1730	Clinical	USA	ERS784917	ERS4267848	GCA_902829545	70 (Illumina)	8.54	68.12	793,173
Burkholderia gladioli	BCC1731	Clinical	USA	ERS784997	ERS4267849	GCA_902831635	62 (Illumina)	8.50	67.97	339,104
Burkholderia gladioli	BCC1732	Clinical	USA	ERS784933	ERS4267850	GCA_902829585	69 (Illumina)	8.82	67.67	606,420
Burkholderia gladioli	BCC1733	Clinical	USA	ERS784951	ERS4267851	GCA_902831575	73 (Illumina)	8.47	68.03	519,208
Burkholderia gladioli	BCC1734	Clinical	USA	ERS785079	ERS4267852	GCA_902831585	78 (Illumina)	8.20	68.24	757,647
Burkholderia gladioli	BCC1735	Clinical	USA	ERS785008	ERS4267853	GCA_902831665	69 (Illumina)	7.93	67.98	388,060
Burkholderia gladioli	BCC1754	Clinical	USA	ERS1371583	ERS4267854	GCA_902829365	64 (Illumina)	8.38	68.20	416,499
Burkholderia gladioli	BCC1755	Clinical	USA	ERS1328941	ERS4267855	GCA_902831625	67 (Illumina)	8.27	68.20	324,811
Burkholderia gladioli	BCC1756	Clinical	USA	ERS1328753	ERS4267856	GCA_902831655	75 (Illumina)	7.75	68.11	264,066
Burkholderia gladioli	BCC1757	Clinical	USA	ERS1371584	ERS4267857	GCA_902831565	70 (Illumina)	8.11	68.25	281,288
Burkholderia gladioli	BCC1758	Clinical	USA	ERS1371585	ERS4267858	GCA_902831615	77 (Illumina)	8.42	68.16	347,044
Burkholderia gladioli	BCC1759	Clinical	USA	ERS1333276	ERS4267859	GCA_902831785	71 (Illumina)	8.42	67.93	202,663
Burkholderia gladioli	BCC1760	Clinical	USA	ERS1371586	ERS4267860	GCA_902831885	74 (Illumina)	8.15	68.21	351,231
Burkholderia gladioli	BCC1761	Clinical	USA	ERS1371587	ERS4267861	GCA_902831865	72 (Illumina)	8.11	68.15	227,067
Burkholderia gladioli	BCC1762	Clinical	USA	ERS1328756	ERS4267862	GCA_902831745	78 (Illumina)	8.38	67.86	232,045
Burkholderia gladioli	BCC1763	Clinical	USA	ERS1328942	ERS4267863	GCA_902831845	70 (Illumina)	8.73	68.08	409,905
Burkholderia gladioli	BCC1764	Clinical	USA	ERS1371588	ERS4267864	GCA_902831775	76 (Illumina)	7.95	68.19	273,951
Burkholderia gladioli	BCC1765	Clinical	USA	ERS1328883	ERS4267865	GCA_902831855	76 (Illumina)	8.06	68.10	267,531
Burkholderia gladioli	BCC1766	Clinical	USA	ERS1371589	ERS4267866	GCA_902831735	77 (Illumina)	8.28	67.89	275,070
Burkholderia gladioli	BCC1767	Clinical	USA	ERS1371590	ERS4267867	GCA_902831875	79 (Illumina)	8.15	68.28	365,649
Burkholderia gladioli	BCC1768	Clinical	USA	ERS1371591	ERS4267868	GCA_902831905	71 (Illumina)	8.26	68.11	226,248
Burkholderia gladioli	BCC1769	Clinical	USA	ERS1328884	ERS4267869	GCA_902831815	67 (Illumina)	8.37	68.02	248,528
Burkholderia gladioli	BCC1770	Clinical	USA	ERS1328772	ERS4267870	GCA_902831755	61 (Illumina)	8.42	67.98	261,993
Burkholderia gladioli	BCC1771	Clinical	USA	ERS1328943	ERS4267871	GCA_902831805	58 (Illumina)	8.41	67.99	303,762
Burkholderia gladioli	BCC1772	Clinical	USA	ERS1328773	ERS4267872	GCA_902831895	58 (Illumina)	8.44	68.03	340,707
Burkholderia gladioli	BCC1773	Clinical	USA	ERS1328885	ERS4267873	GCA_902829405	55 (Illumina)	8.08	68.21	264,067
Burkholderia gladioli	BCC1774	Clinical	USA	ERS1371592	ERS4267874	GCA_902831835	70 (Illumina)	7.89	68.10	264,045
Burkholderia gladioli	BCC1775	Clinical	USA	ERS1333277	ERS4267875	GCA_902831915	65 (Illumina)	8.38	68.13	332,589
Burkholderia gladioli	BCC1777	Clinical	USA	ERS1328886	ERS4267877	GCA_902831795	67 (Illumina)	7.93	68.09	275,756
Burkholderia gladioli	BCC1778	Clinical	USA	ERS1328776	ERS4267878	GCA_902831825	66 (Illumina)	8.30	68.04	284,651
Burkholderia gladioli	BCC1779	Clinical	USA	ERS1328944	ERS4267879	GCA_902831765	65 (Illumina)	8.27	68.02	190,616
Burkholderia gladioli	BCC1780	Clinical	USA	ERS1328777	ERS4267880	GCA_902831945	72 (Illumina)	8.45	67.76	220,441
Burkholderia gladioli	BCC1781	Clinical	USA	ERS1328887	ERS4267881	GCA_902831975	69 (Illumina)	7.99	68.16	208,905
Burkholderia gladioli	BCC1782	Clinical	USA	ERS1328778	ERS4267882	GCA_902829555	62 (Illumina)	8.00	68.24	264,129
Burkholderia gladioli	BCC1783	Clinical	USA	ERS1336060	ERS4267883	GCA_902829505	61 (Illumina)	8.39	67.96	224,024
Burkholderia gladioli	BCC1784	Clinical	USA	ERS1328888	ERS4267884	GCA_902831925	77 (Illumina)	8.13	68.17	273,802
Burkholderia gladioli	BCC1785	Clinical	USA	ERS1328779	ERS4267885	GCA_902831985	75 (Illumina)	7.99	68.26	243,147
Burkholderia gladioli	BCC1786	Clinical	USA	ERS1328945	ERS4267886	GCA_902831965	57 (Illumina)	8.33	68.18	347,752
Burkholderia gladioli	BCC1787	Clinical	USA	ERS1371593	ERS4267887	GCA_902831955	96 (Illumina)	8.25	68.27	371,296
Burkholderia gladioli	BCC1788	Clinical	USA	ERS1371594	ERS4267888	GCA_902831935	68 (Illumina)	8.66	67.84	291,652
Burkholderia gladioli	BCC1789	Clinical	USA	ERS1328781	ERS4267889	GCA_902830195	61 (Illumina)	8.33	68.05	274,262
Burkholderia gladioli	BCC1790	Clinical	USA	ERS1333278	ERS4267890	GCA_902831995	78 (Illumina)	8.41	67.90	249,452
Burkholderia gladioli	BCC1791	Clinical	USA	ERS1328782	ERS4267891	GCA_902832115	68 (Illumina)	8.15	68.14	334,966
Burkholderia gladioli	BCC1792	Clinical	USA	ERS1328890	ERS4267892	GCA_902832095	62 (Illumina)	8.13	68.17	276,382
Burkholderia gladioli	BCC1793	Clinical	USA	ERS1328783	ERS4267893	GCA_902832155	73 (Illumina)	8.14	68.24	280,591
Burkholderia gladioli	BCC1794	Clinical	USA	ERS1328946	ERS4267894	GCA_902832105	70 (Illumina)	8.34	68.23	281,967
Burkholderia gladioli	BCC1795	Clinical	USA	ERS1328784	ERS4267895	GCA_902832035	77 (Illumina)	7.82	68.17	275,050
Burkholderia gladioli	BCC1796	Clinical	USA	ERS1328891	ERS4267896	GCA_902832055	66 (Illumina)	8.38	68.08	351,151
Burkholderia gladioli	BCC1798	Clinical	USA	ERS1371596	ERS4267897	GCA_902832005	75 (Illumina)	8.25	68.07	230,356
Burkholderia gladioli	BCC1799	Clinical	USA	ERS1328786	ERS4267898	GCA_902832015	57 (Illumina)	8.11	68.22	343,906
Burkholderia gladioli	BCC1800	Clinical	USA	ERS1328892	ERS4267899	GCA_902832165	84 (Illumina)	7.92	68.29	257,500
Burkholderia gladioli	BCC1801	Clinical	USA	ERS1328787	ERS4267900	GCA_902832175	75 (Illumina)	7.96	68.24	316,850
Burkholderia gladioli	BCC1802	Clinical	USA	ERS1328947	ERS4267901	GCA_902832185	56 (Illumina)	8.45	68.11	270,804
Burkholderia gladioli	BCC1803	Clinical	USA	ERS1371597	ERS4267902	GCA_902832025	81 (Illumina)	7.71	68.12	272,468
Burkholderia gladioli	BCC1804	Clinical	USA	ERS1371598	ERS4267903	GCA_902832195	71 (Illumina)	8.45	68.00	250,984
Burkholderia gladioli	BCC1805	Clinical	USA	ERS1328789	ERS4267904	GCA_902832045	78 (Illumina)	7.86	67.94	270,833
Burkholderia gladioli	BCC1806	Clinical	USA	ERS1333279	ERS4267905	GCA_902832075	63 (Illumina)	8.48	68.05	273,269
Burkholderia gladioli	BCC1807	Clinical	USA	ERS1371599	ERS4267906	GCA_902832085	69 (Illumina)	8.47	68.00	304,296
Burkholderia gladioli	BCC1808	Clinical	USA	ERS1371600	ERS4267907	GCA_902832145	77 (Illumina)	7.97	68.22	293,939
Burkholderia gladioli	BCC1809	Clinical	USA	ERS1328791	ERS4267908	GCA_902832125	66 (Illumina)	8.64	67.87	235,923
Burkholderia gladioli	BCC1810	Clinical	USA	ERS1328948	ERS4267909	GCA_902830185	64 (Illumina)	8.28	68.22	310,714
Burkholderia gladioli	BCC1811	Clinical	USA	ERS1328792	ERS4267910	GCA_902832135	57 (Illumina)	8.61	67.93	305,514
Burkholderia gladioli	BCC1812	Clinical	USA	ERS1328895	ERS4267911	GCA_902832065	73 (Illumina)	8.23	67.94	301,688
Burkholderia gladioli	BCC1813	Clinical	USA	ERS1328793	ERS4267912	GCA_902832315	65 (Illumina)	8.57	68.01	293,202
Burkholderia gladioli	BCC1814	Clinical	USA	ERS1336062	ERS4267913	GCA_902832285	62 (Illumina)	8.21	68.14	206,859
Burkholderia gladioli	BCC1815	Clinical	USA	ERS1328794	ERS4267914	GCA_902832295	50 (Illumina)	8.95	67.39	262,346
Burkholderia gladioli	BCC1816	Clinical	Canada	ERS1328896	ERS4267915	GCA_902832395	70 (Illumina)	8.18	68.12	326,051
Burkholderia gladioli	BCC1817	Clinical	USA	ERS1328795	ERS4267916	GCA_902832305	69 (Illumina)	8.12	68.21	242,936
Burkholderia gladioli	BCC1818	Clinical	USA	ERS1333280	ERS4267917	GCA_902832365	56 (Illumina)	8.16	68.23	345,879
Burkholderia gladioli	BCC1819	Clinical	USA	ERS1328796	ERS4267918	GCA_902832325	61 (Illumina)	8.42	67.90	331,109
Burkholderia gladioli	BCC1820	Clinical	USA	ERS1328897	ERS4267919	GCA_902832245	75 (Illumina)	8.05	68.15	242,112
Burkholderia gladioli	BCC1821	Clinical	USA	ERS1328797	ERS4267920	GCA_902829485	76 (Illumina)	8.13	68.08	263,017
Burkholderia gladioli	BCC1822	Clinical	USA	ERS1336063	ERS4267921	GCA_902832255	71 (Illumina)	8.08	68.18	402,601
Burkholderia gladioli	BCC1823	Clinical	USA	ERS1328798	ERS4267922	GCA_902829525	69 (Illumina)	8.22	68.31	336,871
Paraburkholderia caribensis	BCC1824	Clinical	USA	ERS1328898	ERS4267923	GCA_902833585	64 (Illumina)	9.04	62.49	121,444
Burkholderia gladioli	BCC1825	Clinical	USA	ERS1328799	ERS4267924	GCA_902832355	70 (Illumina)	8.21	67.96	211,609
Burkholderia gladioli	BCC1826	Clinical	USA	ERS1328949	ERS4267925	GCA_902832385	58 (Illumina)	8.38	68.13	338,267
Burkholderia gladioli	BCC1827	Clinical	USA	ERS1328800	ERS4267926	GCA_902832345	58 (Illumina)	8.71	67.88	269,894
Burkholderia gladioli	BCC1828	Clinical	Canada	ERS1328899	ERS4267927	GCA_902832375	78 (Illumina)	8.24	68.04	268,035
Burkholderia gladioli	BCC1829	Clinical	USA	ERS1371601	ERS4267928	GCA_902832235	80 (Illumina)	8.61	67.80	317,992
Burkholderia gladioli	BCC1830	Clinical	Canada	ERS1371602	ERS4267929	GCA_902832225	76 (Illumina)	8.40	68.05	419,764
Burkholderia gladioli	BCC1831	Clinical	Canada	ERS1371603	ERS4267930	GCA_902832205	74 (Illumina)	8.43	68.00	189,469
Burkholderia gladioli	BCC1832	Clinical	USA	ERS1371604	ERS4267931	GCA_902832215	73 (Illumina)	8.16	68.08	208,866
Burkholderia gladioli	BCC1833	Clinical	USA	ERS1328803	ERS4267932	GCA_902832275	70 (Illumina)	8.49	68.02	3902,44
Burkholderia gladioli	BCC1834	Clinical	USA	ERS1328950	ERS4267933	GCA_902832265	66 (Illumina)	7.90	68.06	286,322
Burkholderia gladioli	BCC1835	Clinical	USA	ERS1371605	ERS4267934	GCA_902832335	73 (Illumina)	8.41	67.96	264,845
Burkholderia gladioli	BCC1836	Clinical	USA	ERS1328901	ERS4267935	GCA_902832445	63 (Illumina)	8.18	68.30	407,323
Burkholderia gladioli	BCC1837	Clinical	USA	ERS1371606	ERS4267936	GCA_902832455	77 (Illumina)	8.14	67.99	206,652
Burkholderia gladioli	BCC1838	Clinical	USA	ERS1336064	ERS4267937	GCA_902830215	68 (Illumina)	8.44	67.91	179,624
Burkholderia gladioli	BCC1840	Clinical	USA	ERS1328902	ERS4267938	GCA_902832405	71 (Illumina)	8.15	68.14	209,087
Burkholderia gladioli	BCC1841	Clinical	USA	ERS1328807	ERS4267939	GCA_902832435	55 (Illumina)	8.72	67.94	274,939
Burkholderia gladioli	BCC1842	Clinical	USA	ERS1328951	ERS4267940	GCA_902832415	76 (Illumina)	8.17	68.27	344,808
Burkholderia gladioli	BCC1843	Clinical	USA	ERS1328808	ERS4267941	GCA_902832425	67 (Illumina)	8.01	68.10	177,545
Burkholderia gladioli	BCC1844	Clinical	USA	ERS1328903	ERS4267942	GCA_902832555	71 (Illumina)	8.20	67.98	219,436
Burkholderia gladioli	BCC1845	Clinical	USA	ERS1328809	ERS4267943	GCA_902832535	71 (Illumina)	8.13	68.24	362,515
Burkholderia gladioli	BCC1846	Clinical	USA	ERS1371607	ERS4267944	GCA_902832475	68 (Illumina)	8.41	68.00	320,953
Burkholderia gladioli	BCC1847	Clinical	USA	ERS1371608	ERS4267945	GCA_902830245	65 (Illumina)	8.41	68.17	287,220
Burkholderia gladioli	BCC1848	Clinical	USA	ERS1328904	ERS4267946	GCA_902832525	69 (Illumina)	8.14	68.04	301,164
Burkholderia gladioli	BCC1849	Clinical	USA	ERS1371609	ERS4267947	GCA_902832485	94 (Illumina)	8.22	68.29	368,142
Burkholderia gladioli	BCC1850	Clinical	USA	ERS1371610	ERS4267948	GCA_902832585	81 (Illumina)	8.53	68.02	275,270
Burkholderia gladioli	BCC1851	Clinical	USA	ERS1371611	ERS4267949	GCA_902832495	65 (Illumina)	8.37	68.20	304,291
Burkholderia gladioli	BCC1852	Clinical	USA	ERS1371612	ERS4267950	GCA_902832515	72 (Illumina)	8.42	68.05	333,375
Burkholderia gladioli	BCC1853	Clinical	USA	ERS1328813	ERS4267951	GCA_902832545	66 (Illumina)	8.22	68.20	326,166
Burkholderia gladioli	BCC1854	Clinical	USA	ERS1371613	ERS4267952	GCA_902832465	69 (Illumina)	8.90	67.81	274,690
Burkholderia gladioli	BCC1855	Clinical	USA	ERS1371614	ERS4267953	GCA_902832505	64 (Illumina)	8.32	68.01	186,469
Burkholderia gladioli	BCC1856	Clinical	USA	ERS1328906	ERS4267954	GCA_902832565	67 (Illumina)	8.05	68.29	286,277
Burkholderia gladioli	BCC1857	Clinical	USA	ERS1371615	ERS4267955	GCA_902832595	68 (Illumina)	8.23	68.25	280,379
Burkholderia gladioli	BCC1858	Clinical	USA	ERS1371616	ERS4267956	GCA_902832575	54 (Illumina)	8.19	68.22	307,394
Burkholderia gladioli	BCC1859	Clinical	USA	ERS1371617	ERS4267957	GCA_902832615	95 (Illumina)	8.26	68.20	334,836
Burkholderia gladioli	BCC1860	Clinical	USA	ERS1371618	ERS4267958	GCA_902832655	88 (Illumina)	8.63	68.00	347,760
Burkholderia gladioli	BCC1861	Clinical	USA	ERS1371619	ERS4267959	GCA_902829615	79 (Illumina)	8.63	67.75	216,674
Burkholderia gladioli	BCC1862	Clinical	USA	ERS1371620	ERS4267960	GCA_902832685	89 (Illumina)	8.38	68.14	294,407
Burkholderia gladioli	BCC1863	Clinical	USA	ERS1371621	ERS4267961	GCA_902832605	74 (Illumina)	8.27	68.23	294,023
Burkholderia gladioli	BCC1864	Clinical	USA	ERS1328908	ERS4267962	GCA_902830235	53 (Illumina)	8.50	67.90	212,901
Burkholderia gladioli	BCC1865	Clinical	USA	ERS1328819	ERS4267963	GCA_902832725	72 (Illumina)	8.23	68.15	233,312
Burkholderia gladioli	BCC1866	Clinical	USA	ERS1371622	ERS4267964	GCA_902832695	71 (Illumina)	8.46	67.99	287,257
Burkholderia gladioli	BCC1867	Clinical	USA	ERS1371623	ERS4267965	GCA_902832675	72 (Illumina)	8.40	67.96	178,703
Burkholderia gladioli	BCC1868	Clinical	USA	ERS1371624	ERS4267966	GCA_902832715	62 (Illumina)	7.93	68.19	393,583
Burkholderia gladioli	BCC1869	Clinical	USA	ERS1328821	ERS4267967	GCA_902832665	64 (Illumina)	8.60	67.92	311,968
Burkholderia gladioli	BCC1870	Clinical	USA	ERS1371625	ERS4267968	GCA_902832645	86 (Illumina)	8.35	67.95	186,380
Burkholderia gladioli	BCC1871	Clinical	USA	ERS1371626	ERS4267969	GCA_902832705	95 (Illumina)	8.06	68.11	284,538
Burkholderia gladioli	BCC1872	Clinical	USA	ERS1371627	ERS4267970	GCA_902832745	74 (Illumina)	8.43	68.15	286,407
Burkholderia gladioli	BCC1880			ERS1371628	ERS4267971	GCA_902832635	73 (Illumina)	8.23	68.04	621,809
Burkholderia gladioli	BCC1881			ERS1371629	ERS4267972	GCA_902832735	71 (Illumina)	8.39	68.20	350,910
Burkholderia gladioli	BCC1882			ERS1371630	ERS4267973	GCA_902832625	58 (Illumina)	8.39	68.20	350,910
Burkholderia gladioli	BCC1883			ERS1371631	ERS4267974	GCA_902832785	71 (Illumina)	8.13	68.31	603,194
Burkholderia glumae	LMG 2196	Environmental, Oryza sativa	Japan	ERS784969	ERS4267975	GCA_902832765	92 (Illumina)	6.66	68.35	162,494
Burkholderia lata	BCC0174	Environmental, soil	Trinidad and Tobago	ERS785040	ERS4267976	GCA_902832775	63 (Illumina)	8.48	66.57	1,023,694
Burkholderia sp.	BCC0217	Clinical, cystic fibrosis patient	USA	ERS784822	ERS4267977	GCA_902833005	74 (Illumina)	9.44	65.63	453,341
Burkholderia latens	BCC1625	Clinical, cystic fibrosis patient	Italy	ERS784839	ERS4267978	GCA_902832795	80 (Illumina)	7.08	66.83	469,972
Burkholderia latens	BCC1626	Clinical, cystic fibrosis patient	UK	ERS784855	ERS4267979	GCA_902832755	78 (Illumina)	7.25	66.69	638,063
Burkholderia metallica	BCC0095	Clinical, sputum	Canada	ERS785019	ERS4267980	GCA_902832815	67 (Illumina)	7.75	66.76	474,034
Burkholderia metallica	BCC0483	Clinical		ERS784872	ERS4267981	GCA_902832805	75 (Illumina)	7.55	67.06	469,713
Burkholderia metallica	LMG 24068	Clinical, cystic fibrosis patient	USA	ERS784887	ERS4267982	GCA_902832845	75 (Illumina)	7.55	67.06	476,026
Burkholderia multivorans	BCC0005	Clinical, sputum	Canada	ERS785011	ERS4267983	GCA_902832835	87 (Illumina)	6.39	67.37	1,402,971
Burkholderia multivorans	BCC0008	Clinical, sputum	UK	ERS784904	ERS4267984	GCA_902832825	88 (Illumina)	6.63	67.18	793,813
Burkholderia multivorans	BCC0010	Clinical, non-cystic fibrosis patient brain abscess	UK	ERS784919	ERS4267985	GCA_902833875	92 (Illumina)	6.27	67.24	486,046
Burkholderia multivorans	BCC0031	Clinical, sputum	Canada	ERS785002	ERS4267986	GCA_902833805	89 (Illumina)	6.53	67.07	561,216
Burkholderia multivorans	BCC0037	Clinical, chronic granulomatous disease, lung	USA	ERS785053	ERS4267987	GCA_902833915	93 (Illumina)	6.56	67.16	303,874
Burkholderia multivorans	BCC0050	Environmental, soil	UK	ERS785036	ERS4267988	GCA_902833885	78 (Illumina)	6.97	66.86	531,523
Burkholderia multivorans	BCC0059	Clinical, sputum	Canada	ERS785022	ERS4267989	GCA_902833835	74 (Illumina)	6.76	67.09	384,200
Burkholderia multivorans	BCC0067	Clinical, sputum	Canada	ERS784935	ERS4267990	GCA_902833855	96 (Illumina)	6.12	67.36	349,267
Burkholderia multivorans	BCC0089	Clinical, sputum	Canada	ERS785033	ERS4267991	GCA_902833895	82 (Illumina)	6.39	67.21	595,797
Burkholderia multivorans	BCC0093	Clinical, respiratory sample	Canada	ERS785061	ERS4267992	GCA_902832975	96 (Illumina)	6.30	67.22	342,629
Burkholderia multivorans	BCC0099	Clinical, respiratory sample	Canada	ERS785069	ERS4267993	GCA_902832855	94 (Illumina)	6.93	66.81	276,171
Burkholderia multivorans	BCC0102	Clinical, respiratory sample	Canada	ERS784954	ERS4267994	GCA_902833015	80 (Illumina)	6.66	67.18	459,105
Burkholderia multivorans	BCC0115	Clinical, sputum	USA	ERS784971	ERS4267995	GCA_902830305	102 (Illumina)	6.22	67.36	413,909
Burkholderia multivorans	BCC0149	Clinical, cystic fibrosis patient	USA	ERS785050	ERS4267996	GCA_902832965	86 (Illumina)	6.35	67.17	436,404
Burkholderia multivorans	BCC0175	Clinical, chronic granulomatous disease, blood	USA	ERS785024	ERS4267997	GCA_902832875	77 (Illumina)	6.71	66.72	328,297
Burkholderia multivorans	BCC0244	Clinical, sputum	Canada	ERS785004	ERS4267998	GCA_902832915	93 (Illumina)	6.57	67.18	435,992
Burkholderia oklahomensis	LMG 23618	Environmental		ERS784979	ERS4267999	GCA_902829515	88 (Illumina)	7.09	67.11	364,599
Burkholderia plantarii	LMG 9035	Environmental, Oryza sativa cv. Koshihikari, seedling with blight and chlorosis	Japan	ERS784824	ERS4268000	GCA_902832905	79 (Illumina)	8.04	68.61	305,422
Burkholderia pyrrocinia	BCC0171	Environmental, cornfield soil	USA	ERS785059	ERS4268001	GCA_902832995	66 (Illumina)	8.16	66.18	325,972
Burkholderia pyrrocinia	LMG 14191	Environmental, soil		ERS785083	ERS4268002	GCA_902832895	75 (Illumina)	7.86	66.51	520,567
Burkholderia pyrrocinia	BCC0986	Environmental		ERS784857	ERS4268003	GCA_902829605	74 (Illumina)	7.60	66.70	556,419
Burkholderia pyrrocinia	BCC1346	Environmental		ERS784841	ERS4268004	GCA_902832935	70 (Illumina)	8.34	65.94	282,972
Burkholderia pyrrocinia	BCC1364	Clinical		ERS784874	ERS4268005	GCA_902832945	75 (Illumina)	7.62	66.57	587,939
Burkholderia seminalis	LMG 24067	Clinical, cystic fibrosis patient	USA	ERS784889	ERS4268006	GCA_902832885	70 (Illumina)	7.97	67.09	523,234
Burkholderia seminalis	BCC1628	Environmental, Oryza sativa seed	Philippines	ERS784906	ERS4268007	GCA_902833035	60 (Illumina)	8.24	66.71	331,981
Burkholderia sp.	BCC1642	Environmental		ERS1328853	ERS4268008	GCA_902829715	78 (Illumina)	9.22	62.08	159,099
Burkholderia stabilis	BCC0186	Clinical, cystic fibrosis patient	Belgium	ERS785081	ERS4268009	GCA_902833135	92 (Illumina)	7.15	66.55	267,680
Burkholderia stabilis	BCC0237	Environmental, albuterol inhaler	USA	ERS785013	ERS4268010	GCA_902833115	58 (Illumina)	8.52	66.46	323,418
Burkholderia stabilis	BCC0285	Clinical, infected tissue of tibial fracture		ERS784949	ERS4268011	GCA_902833105	80 (Illumina)	8.75	66.23	274,531
Burkholderia stabilis	BCC1308	Environmental/industrial		ERS784921	ERS4268012	GCA_902833185	61 (Illumina)	8.25	66.51	685,734
Burkholderia stagnalis	LMG 28156	Environmental, soil	Australia	ERS1371647	ERS4268013	GCA_902830275	66 (Illumina)	8.03	67.24	262,379
Burkholderia territorii	LMG 28158	Environmental, water	Australia	ERS1328858	ERS4268014	GCA_902833055	92 (Illumina)	6.98	66.66	277,911
Burkholderia ubonensis	LMG 20358	Environmental, surface soil	Thailand	ERS784956	ERS4268015	GCA_902833085	66 (Illumina)	7.77	67.30	623,290
Burkholderia vietnamiensis	BCC0027	Clinical, cystic fibrosis patient	USA	ERS785078	ERS4268016	GCA_902833235	85 (Illumina)	6.87	66.94	283,796
Burkholderia vietnamiensis	BCC0028	Clinical, cystic fibrosis patient	Sweden	ERS785077	ERS4268017	GCA_902833215	94 (Illumina)	6.67	67.25	195,580
Burkholderia vietnamiensis	BCC0029	Clinical, chronic granulomatous disease, lung abscess	Canada	ERS784991	ERS4268018	GCA_902829455	95 (Illumina)	6.98	66.73	204,529
Burkholderia vietnamiensis	LMG 10929	Environmental, Oryza sativa rhizosphere soil	Vietnam	ERS785016	ERS4268019	GCA_902830295	75 (Illumina)	6.88	66.90	308,470
Burkholderia vietnamiensis	BCC0040	Clinical, non-cystic fibrosis patient		ERS784999	ERS4268020	GCA_902833385	89 (Illumina)	6.26	66.68	397,866
Burkholderia vietnamiensis	BCC0042	Environmental, hospital		ERS784972	ERS4268021	GCA_902833285	105 (Illumina)	6.29	66.65	667,227
Burkholderia vietnamiensis	BCC0046	Clinical, non-cystic fibrosis patient		ERS785044	ERS4268022	GCA_902833275	81 (Illumina)	6.77	66.97	511,799
Burkholderia vietnamiensis	BCC0104	Clinical, lymph node	USA	ERS785007	ERS4268023	GCA_902833335	74 (Illumina)	7.06	66.85	228,863
Burkholderia vietnamiensis	BCC0124	Clinical, sputum	USA	ERS785030	ERS4268024	GCA_902833355	73 (Illumina)	7.11	66.93	309,681
Burkholderia vietnamiensis	BCC0126	Clinical, sputum	USA	ERS1328823	ERS4268025	GCA_902833375	72 (Illumina)	6.81	67.10	186,133
Burkholderia vietnamiensis	BCC0128	Clinical, sputum	Canada	ERS784980	ERS4268026	GCA_902833415	96 (Illumina)	6.63	67.17	400,883
Burkholderia vietnamiensis	BCC0129	Clinical	USA	ERS1328846	ERS4268027	GCA_902833445	79 (Illumina)	6.98	66.46	186,188
Burkholderia vietnamiensis	BCC0136	Clinical, throat	Canada	ERS785029	ERS4268028	GCA_902833435	69 (Illumina)	7.29	66.68	225,055
Burkholderia vietnamiensis	BCC0142	Clinical	USA	ERS784988	ERS4268029	GCA_902833265	86 (Illumina)	7.21	66.73	246,894
Burkholderia vietnamiensis	BCC0151	Clinical	USA	ERS784826	ERS4268030	GCA_902833395	95 (Illumina)	6.08	67.33	168,450
Burkholderia vietnamiensis	BCC0157	Clinical, sputum	USA	ERS1371645	ERS4268031	GCA_902833295	102 (Illumina)	6.35	67.18	137,809
Burkholderia vietnamiensis	BCC0185	Clinical, neck abscess	Chile	ERS1336070	ERS4268032	GCA_902833315	77 (Illumina)	7.12	66.80	202,960
Burkholderia vietnamiensis	BCC0190	Clinical	USA	ERS785015	ERS4268033	GCA_902830265	84 (Illumina)	6.16	66.76	523,281
Burkholderia vietnamiensis	BCC0194	Environmental, soil		ERS785075	ERS4268034	GCA_902833365	75 (Illumina)	7.06	66.93	158,113
Burkholderia vietnamiensis	BCC0324	Clinical, non-cystic fibrosis patient		ERS784843	ERS4268035	GCA_902833345	92 (Illumina)	6.21	66.62	311,192
Burkholderia vietnamiensis	BCC0587	Environmental, soil		ERS1328927	ERS4268037	GCA_902833305	71 (Illumina)	7.39	66.79	187,227
Burkholderia vietnamiensis	BCC1161	Clinical		ERS785038	ERS4268038	GCA_902833255	78 (Illumina)	6.62	67.33	306,697
Burkholderia vietnamiensis	BCC1162	Clinical		ERS784859	ERS4268039	GCA_902833325	85 (Illumina)	6.62	67.33	308,591
Burkholderia vietnamiensis	BCC1163	Clinical		ERS784876	ERS4268040	GCA_902833545	81 (Illumina)	6.62	67.33	314,533
Burkholderia vietnamiensis	BCC1170	Clinical		ERS785023	ERS4268041	GCA_902833495	79 (Illumina)	6.91	66.39	157,040
Burkholderia vietnamiensis	BCC1172	Environmental		ERS785031	ERS4268042	GCA_902833625	71 (Illumina)	7.39	66.30	161,339
Burkholderia vietnamiensis	BCC1176	Clinical		ERS785046	ERS4268043	GCA_902833605	76 (Illumina)	7.09	66.83	457,880
Burkholderia vietnamiensis	BCC1186	Clinical		ERS784891	ERS4268044	GCA_902833535	72 (Illumina)	7.08	66.45	138,269
Burkholderia vietnamiensis	BCC1193	Clinical		ERS785058	ERS4268045	GCA_902833555	72 (Illumina)	7.25	66.29	160,650
Burkholderia vietnamiensis	BCC1301	Environmental/industrial		ERS785065	ERS4268046	GCA_902833615	80 (Illumina)	7.05	66.78	246,898
Burkholderia vietnamiensis	BCC1304	Environmental/industrial		ERS784990	ERS4268047	GCA_902833475	71 (Illumina)	8.09	66.11	246,358
Burkholderia vietnamiensis	BCC1309	Environmental/industrial		ERS785063	ERS4268048	GCA_902833565	82 (Illumina)	7.08	66.91	276,656
Burkholderia vietnamiensis	BCC1409	Environmental/industrial		ERS785012	ERS4268049	GCA_902833595	72 (Illumina)	7.22	66.67	308,880
Burkholderia vietnamiensis	BCC1412	Environmental/industrial		ERS785020	ERS4268050	GCA_902833465	71 (Illumina)	7.34	66.61	178,163
Burkholderia cepacia	BCC1633	Environmental	Malaysia	ERS1328923	ERS4268051	GCA_902830795	81 (Illumina)	8.84	66.29	275,215
Caballeronia glathei	BCC0772	Environmental, lateritic soil	Germany	ERS784993	ERS4268052	GCA_902833485	75 (Illumina)	8.74	64.38	262,729
Paraburkholderia bryophila	BCC1875	Environmental, isolated from Sphagnum rubellum moss	Germany	ERS1328963	ERS4268053	GCA_902833525	71 (Illumina)	8.09	62.91	200,899
Paraburkholderia sp.	BCC1876	Environmental, isolated from Aulacomnium palustre moss	Germany	ERS1328856	ERS4268054	GCA_902833695	68 (Illumina)	8.50	62.75	785,957
Paraburkholderia caledonica	LMG 19076	Environmental, soil rhizosphere	UK	ERS784802	ERS4268055	GCA_902833635	99 (Illumina)	7.31	61.96	449,131
Paraburkholderia caribensis	LMG 18531	Environmental, soil, vertisol		ERS784810	ERS4268056	GCA_902833515	66 (Illumina)	8.97	62.60	301,636
Paraburkholderia fungorum	LMG 16225	Environmental, Phanerochaete chrysosporium fungus	France	ERS784892	ERS4268057	GCA_902833645	67 (Illumina)	8.93	61.85	451,759
Paraburkholderia hospita	LMG 20598	Environmental, agricultural soil	Belgium	ERS784978	ERS4268058	GCA_902833685	56 (Illumina)	11.25	61.88	202,916
Paraburkholderia kururiensis	LMG 19447	Environmental, aquifer	Japan	ERS784986	ERS4268059	GCA_902833705	84 (Illumina)	7.54	64.83	257,717
Burkholderia vietnamiensis	BCC1878	Environmental, isolated from Aulacomnium palustre moss	Germany	ERS1328857	ERS4268060	GCA_902829795	91 (Illumina)	7.03	66.73	129,098
Paraburkholderia mimosarum	LMG 23256	Environmental, Mimosa pigra root nodule	China	ERS785026	ERS4268061	GCA_902833725	60 (Illumina)	8.60	63.84	198,630
Paraburkholderia phenazinium	BCC1873	Environmental		ERS1328926	ERS4268062	GCA_902829745	70 (Illumina)	8.96	62.26	287,222
Paraburkholderia phenazinium	BCC1874			ERS1328855	ERS4268063	GCA_902833675	71 (Illumina)	9.00	62.28	309,756
Paraburkholderia phenoliruptrix	LMG 22037	Environmental, chemostat		ERS1371595	ERS4268064	GCA_902833655	82 (Illumina)	7.36	63.48	174,050
Paraburkholderia phymatum	LMG 21445	Environmental, Machaerium lunatum root nodule	French Guiana	ERS784987	ERS4268065	GCA_902833665	72 (Illumina)	8.60	62.32	334,756
Paraburkholderia sacchari	LMG 19450	Environmental, sugarcane plantation soil	Brazil	ERS785005	ERS4268066	GCA_902833715	81 (Illumina)	7.27	64.04	428,857
Paraburkholderia terricola	LMG 20594	Environmental, agricultural soil	Belgium	ERS785021	ERS4268067	GCA_902833815	67 (Illumina)	7.34	63.65	179,029
Paraburkholderia tropica	LMG 22274	Environmental, sugarcane roots	Brazil	ERS784938	ERS4268068	GCA_902833865	60 (Illumina)	8.60	64.77	492,153
Paraburkholderia tropica	BCC1637	Environmental	Malaysia	ERS1328924	ERS4268069	GCA_902833825	80 (Illumina)	8.35	64.80	300,108
Paraburkholderia tuberum	LMG 21444	Environmental, Aspalathus carnosa root nodule	South Africa	ERS785070	ERS4268070	GCA_902833905	64 (Illumina)	8.79	63.06	137,501
Robbsia andropogonis	LMG 2129	Environmental, Sorghum bicolor	USA	ERS784901	ERS4268071	GCA_902833845	84 (Illumina)	6.33	58.87	340,675
Trinickia caryophylli	LMG 2155	Environmental, Dianthus caryophyllus	USA	ERS784825	ERS4268072	GCA_902833925	104 (Illumina)	6.56	64.73	645,930
Burkholderia stagnalis	BCC1350	Environmental			ERS4268073	GCA_902833205	150 (PacBio)	8.45	67.06	3,279,208
Burkholderia gladioli	BCC0238	Clinical, sputum	USA		ERS4268074	GCA_902829535	150 (PacBio)	8.64	67.94	4,046,521
Burkholderia vietnamiensis	BCC0268	Environmental, soil	New Zealand		ERS4268075	GCA_902833425	150 (PacBio)	6.81	67.10	2,221,444
Burkholderia vietnamiensis	BCC1408	Environmental/industrial, diesel contaminant	UK		ERS4268076	GCA_902833505	150 (PacBio)	7.89	66.39	1,872,953

The genome collection contains 24 named Burkholderia species and 17 genomes that possessed an ANI value of <95% compared to sequenced type strains. Within the Burkholderia cepacia complex, the first representative genome of Burkholderia arboris has been deposited, as well as genomes for type strains of Burkholderia anthina, Burkholderia metallica, Burkholderia seminalis, Burkholderia territorii, and Burkholderia ubonensis. The collection considerably increases the number of curated Burkholderia genomes, specifically for Burkholderia gladioli (207 genomes), Burkholderia ambifaria (60 genomes), and Burkholderia vietnamiensis (35 genomes).

The Paraburkholderia genomes cover 14 named species and 1 unnamed species based on ANI analysis. The type strains of Paraburkholderia caledonica and Paraburkholderia tuberum were sequenced, and the type strains of 11 species were resequenced. Additional genome assemblies were deposited for Paraburkholderia megapolitana and Paraburkholderia phenazinium. Finally, three Caballeronia genomes were identified in the collection, i.e., Caballeronia glathei, Caballeronia zhejiangensis, and an uncharacterized Caballeronia species. The type strains of Robbsia andropogonis and Trinickia caryophylli were also resequenced.

Multiple novel metabolites have been identified, and their biosynthetic pathways have been elucidated, including the antimycobacterial compound gladiolin (9) and the lipopeptidiolide antibiotic icosalides (15) in B. gladioli. The discovery and biosynthesis of enacyloxin lla, an antibiotic with potent activity against Acinetobacter baumannii (16–18), and the antioomycete metabolite cepacin (19) have been studied in B. ambifaria. Further analysis of this Burkholderiaceae genome resource is under way to identify additional biologically active specialized metabolites and their potential applications.

Data availability.

The genome sequences in this announcement have been deposited in the European Nucleotide Archive (ENA) under the project accession number PRJEB35318. The BioSample accession numbers for the 454 genome assemblies are ERS4267623 to ERS4268076. The Illumina paired-end read data associated with these genome assemblies have been deposited under the project accession number PRJEB9765, with the exception of Burkholderia diffusa LMG 29043, which is available under project accession number PRJEB35318.