Literature DB >> 33058563

Laboratory Maintenance and Propagation of Freshwater Planarians.

Makayla R P Dean1, Elizabeth M Duncan1.   

Abstract

Freshwater planarians are a powerful model organism for the study of animal regeneration, stem cell maintenance and differentiation, and the development and functions of several highly conserved complex tissues. At the same time, planarians are easy to maintain, inexpensive to propagate, and reasonably macroscopic (1 mm to 1 cm in length), making them excellent organisms to use in both complex academic research and hands-on teaching laboratories. Here, we provide a detailed description of how to maintain and propagate these incredibly versatile animals in any basic laboratory setting.
© 2020 Wiley Periodicals LLC. Basic Protocol 1: Salt solution preparation Basic Protocol 2: Cleaning planarian housing Basic Protocol 3: Food preparation Basic Protocol 4: Feeding planarians Basic Protocol 5: Expansion and amplification of colony. © 2020 Wiley Periodicals LLC.

Entities:  

Keywords:  Girardia; Schmidtea mediterranea; planarian; regeneration

Mesh:

Substances:

Year:  2020        PMID: 33058563      PMCID: PMC7941200          DOI: 10.1002/cpmc.120

Source DB:  PubMed          Journal:  Curr Protoc Microbiol        ISSN: 1934-8525


  15 in total

1.  Decreased neoblast progeny and increased cell death during starvation-induced planarian degrowth.

Authors:  Cristina González-Estévez; Daniel A Felix; Gustavo Rodríguez-Esteban; A Aziz Aboobaker
Journal:  Int J Dev Biol       Date:  2012       Impact factor: 2.203

2.  The planarian Schmidtea mediterranea as a model for studying motile cilia and multiciliated cells.

Authors:  Cyril Basquin; Anne-Marie Orfila; Juliette Azimzadeh
Journal:  Methods Cell Biol       Date:  2015-03-07       Impact factor: 1.441

Review 3.  Evolution and regeneration of the planarian central nervous system.

Authors:  Yoshihiko Umesono; Kiyokazu Agata
Journal:  Dev Growth Differ       Date:  2009-04       Impact factor: 2.053

4.  Genetic and physiologic control of fissioning and sexuality in planarians.

Authors:  M Grasso; M Benazzi
Journal:  J Embryol Exp Morphol       Date:  1973-10

5.  The molecular logic for planarian regeneration along the anterior-posterior axis.

Authors:  Yoshihiko Umesono; Junichi Tasaki; Yui Nishimura; Martina Hrouda; Eri Kawaguchi; Shigenobu Yazawa; Osamu Nishimura; Kazutaka Hosoda; Takeshi Inoue; Kiyokazu Agata
Journal:  Nature       Date:  2013-07-24       Impact factor: 49.962

6.  Reactivating head regrowth in a regeneration-deficient planarian species.

Authors:  S-Y Liu; C Selck; B Friedrich; R Lutz; M Vila-Farré; A Dahl; H Brandl; N Lakshmanaperumal; I Henry; J C Rink
Journal:  Nature       Date:  2013-07-24       Impact factor: 49.962

7.  The maintenance and regeneration of the planarian excretory system are regulated by EGFR signaling.

Authors:  Jochen C Rink; Hanh Thi-Kim Vu; Alejandro Sánchez Alvarado
Journal:  Development       Date:  2011-09       Impact factor: 6.868

Review 8.  The history and enduring contributions of planarians to the study of animal regeneration.

Authors:  Sarah A Elliott; Alejandro Sánchez Alvarado
Journal:  Wiley Interdiscip Rev Dev Biol       Date:  2012-07-23       Impact factor: 5.814

Review 9.  The freshwater planarian Schmidtea mediterranea: embryogenesis, stem cells and regeneration.

Authors:  Alejandro Sánchez Alvarado
Journal:  Curr Opin Genet Dev       Date:  2003-08       Impact factor: 5.578

10.  Wnt and TGFβ coordinate growth and patterning to regulate size-dependent behaviour.

Authors:  Christopher P Arnold; Blair W Benham-Pyle; Jeffrey J Lange; Christopher J Wood; Alejandro Sánchez Alvarado
Journal:  Nature       Date:  2019-08-15       Impact factor: 49.962

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