S Mrakic-Sposta1, M Gussoni2, C Dellanoce3, M Marzorati4, M Montorsi5, L Rasica4,6, L Pratali7, G D'Angelo7, M Martinelli8, L Bastiani7, L Di Natale9, A Vezzoli3. 1. Institute of Clinical Physiology, National Research Council (IFC-CNR), Piazza dell'Ospedale Maggiore, 3, 20162, Milan, Italy. simona.mrakicsposta@cnr.it. 2. Institute of Science and Chemical Technology, National Council of Research (SCITEC-CNR), Milan, Italy. 3. Institute of Clinical Physiology, National Research Council (IFC-CNR), Piazza dell'Ospedale Maggiore, 3, 20162, Milan, Italy. 4. Institute of Biomedical Technologies, National Research Council (CNR), Segrate, Milano, Italy. 5. Department of Human Sciences and Promotion of the Quality of Life, San Raffaele Roma Open University, Milan, Italy. 6. Faculty of Kinesiology, University of Calgary, Calgary, Canada. 7. Institute of Clinical Physiology, National Council of Research (IFC-CNR), Pisa, Italy. 8. Institute of Information Science and Technologies, National Research Council (ISTI-CNR), Pisa, Italy. 9. Centre Hospitalier Universitarie de Dijon-Bourgogne, Dijon, France.
Abstract
PURPOSE: High altitude results in lower barometric pressure and hence partial pressure of O2 decrease can lead to several molecular and cellular changes, such as generation of reactive oxygen species (ROS). Electron Paramagnetic Resonance technique was adopted in the field, to evaluate the effects of acute and sub-acute hypobaric hypoxia (HH) on ROS production by micro-invasive method. Biological biomarkers, indicators of oxidative stress, renal function and inflammation were investigated too. METHODS: Fourteen lowlander subjects (mean age 27.3 ± 5.9 years) were exposed to HH at 3269 m s.l. ROS production, related oxidative damage to cellular components, systemic inflammatory response and renal function were determined through blood and urine profile performed at 1st, 2nd, 4th, 7th, and 14th days during sojourn. RESULTS: Kinetics of changes during HH exposition showed out significant (range p < 0.05-0.0001) increases that at max corresponds to 38% for ROS production rate, 140% for protein carbonyl, 44% for lipid peroxidation, 42% for DNA damage, 200% for inflammatory cytokines and modifications in renal function (assessed by neopterin concentration: 48%). Conversely, antioxidant capacity significantly (p < 0.0001) decreased - 17% at max. CONCLUSION: This 14 days in-field study describes changes of oxidative-stress biomarkers during HH exposure in lowlanders. The results show an overproduction of ROS and consequent oxidative damage to protein, lipids and DNA with a decrease in antioxidant capacity and the involvement of inflammatory status and a transient renal dysfunction. Exposure at high altitude induces a hypoxic condition during acute and sub-acute phases accompanied by molecular adaptation mechanism indicating acclimatization.
PURPOSE: High altitude results in lower barometric pressure and hence partial pressure of O2 decrease can lead to several molecular and cellular changes, such as generation of reactive oxygen species (ROS). Electron Paramagnetic Resonance technique was adopted in the field, to evaluate the effects of acute and sub-acute hypobaric hypoxia (HH) on ROS production by micro-invasive method. Biological biomarkers, indicators of oxidative stress, renal function and inflammation were investigated too. METHODS: Fourteen lowlander subjects (mean age 27.3 ± 5.9 years) were exposed to HH at 3269 m s.l. ROS production, related oxidative damage to cellular components, systemic inflammatory response and renal function were determined through blood and urine profile performed at 1st, 2nd, 4th, 7th, and 14th days during sojourn. RESULTS: Kinetics of changes during HH exposition showed out significant (range p < 0.05-0.0001) increases that at max corresponds to 38% for ROS production rate, 140% for protein carbonyl, 44% for lipid peroxidation, 42% for DNA damage, 200% for inflammatory cytokines and modifications in renal function (assessed by neopterin concentration: 48%). Conversely, antioxidant capacity significantly (p < 0.0001) decreased - 17% at max. CONCLUSION: This 14 days in-field study describes changes of oxidative-stress biomarkers during HH exposure in lowlanders. The results show an overproduction of ROS and consequent oxidative damage to protein, lipids and DNA with a decrease in antioxidant capacity and the involvement of inflammatory status and a transient renal dysfunction. Exposure at high altitude induces a hypoxic condition during acute and sub-acute phases accompanied by molecular adaptation mechanism indicating acclimatization.
Entities:
Keywords:
Acclimatization; Electron paramagnetic resonance; Hypobaric hypoxia; Oxidative stress; Reactive oxygen species
Authors: Adam J Chicco; Catherine H Le; Erich Gnaiger; Hans C Dreyer; Jonathan B Muyskens; Angelo D'Alessandro; Travis Nemkov; Austin D Hocker; Jessica E Prenni; Lisa M Wolfe; Nathan M Sindt; Andrew T Lovering; Andrew W Subudhi; Robert C Roach Journal: J Biol Chem Date: 2018-03-14 Impact factor: 5.157
Authors: Ri-Li Ge; Tony G Babb; Mark Sivieri; Geir K Resaland; Trine Karlsen; Jim Stray-Gundersen; Benjamin D Levine Journal: High Alt Med Biol Date: 2006 Impact factor: 1.981
Authors: Tadej Debevec; Vincent Pialoux; Igor B Mekjavic; Ola Eiken; Pauline Mury; Grégoire P Millet Journal: Med Sci Sports Exerc Date: 2014-01 Impact factor: 5.411
Authors: Morgan Levenez; Kate Lambrechts; Simona Mrakic-Sposta; Alessandra Vezzoli; Peter Germonpré; Hadrien Pique; Fabio Virgili; Gerardo Bosco; Pierre Lafère; Costantino Balestra Journal: Int J Environ Res Public Health Date: 2022-01-15 Impact factor: 3.390