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Literature DB >> 3305372

Purification of actin from Candida albicans and comparison with the Candida 48,000-Mr protein.

Abstract

Actin was purified from Candida albicans cells by affinity chromatography by DNase-Sepharose and was recognized by immunoblotting with monoclonal antibody directed against chick muscle actin. The C. albicans 48-kilodalton protein recognized by sera from patients with invasive candidiasis was shown by DEAE chromatography and immunoblotting not to be identical with the purified C. albicans actin.

Entities: Chemical Disease Gene Species

Mesh：

Substances：

Year: 1987 PMID： 3305372 PMCID： PMC260703 DOI： 10.1128/iai.55.9.2324-2326.1987

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

11 in total

1. Fused rocket immunoelectrophoresis.

Authors: P J Svendsen
Journal: Scand J Immunol Suppl Date: 1973

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

3. An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida Albicans.

Authors: K L Lee; H R Buckley; C C Campbell
Journal: Sabouraudia Date: 1975-07

4. Isolation and characterization of actin-like protein from yeast Saccharomyces cerevisiae.

Authors: V E Koteliansky; M A Glukhova; M V Bejanian; A P Surguchov; V N Smirnov
Journal: FEBS Lett Date: 1979-06-01 Impact factor: 4.124

5. Isolation of polymerization-competent cytoplasmic actin by affinity chromatography on immobilized DNAse I using formamide as eluant.

Authors: K Zechel
Journal: Eur J Biochem Date: 1980-09

6. Production and characterization of three monoclonal antibodies to Candida albicans proteins.

Authors: N A Strockbine; M T Largen; H R Buckley
Journal: Infect Immun Date: 1984-03 Impact factor: 3.441

7. Identification and molecular weight characterization of antigens from Candida albicans that are recognized by human sera.

Authors: N A Strockbine; M T Largen; S M Zweibel; H R Buckley
Journal: Infect Immun Date: 1984-02 Impact factor: 3.441

Purification of actin from Candida albicans and comparison with the Candida 48,000-Mr protein.

1. Fused rocket immunoelectrophoresis.

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

3. An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida Albicans.

4. Isolation and characterization of actin-like protein from yeast Saccharomyces cerevisiae.

5. Isolation of polymerization-competent cytoplasmic actin by affinity chromatography on immobilized DNAse I using formamide as eluant.

6. Production and characterization of three monoclonal antibodies to Candida albicans proteins.

7. Identification and molecular weight characterization of antigens from Candida albicans that are recognized by human sera.

8. Identification of an actin-like protein and of its messenger ribonucleic acid in Saccharomyces cerevisiae.

9. Actin is the naturally occurring inhibitor of deoxyribonuclease I.

10. Actin from Saccharomyces cerevisiae.

Review 1. Cytological immunodetection of yeast glycoprotein secretion.

Review 2. Serological techniques for diagnosis of fungal infection.