Characterizing the Transport of Aluminum, Silicon, and Titanium-Containing Particles and Nanoparticles in Mainstream Tobacco Smoke.

The most commonly observed forms of aluminum, silicon, and titanium in tobacco products are aluminum silicates (e.g., kaolin), silica, and titanium(IV) oxide. These compounds are neither water soluble nor volatile at cigarette combustion temperatures. Rather, they are transported in mainstream tobacco smoke as particles after being freed by combustion from the tobacco filler and can induce pulmonary inflammation when inhaled. Aluminum silicate particles are the most frequently observed particles in the pulmonary macrophages of smokers and have become known as "smokers' inclusions." A relatively new technique, single particle triple quadrupole inductively coupled plasma-mass spectrometry (sp-QQQ-ICP-MS) was used to analyze aluminum, silicon, and titanium-containing particle deliveries in cigarette and little cigar mainstream tobacco smoke, and to collect information on solid inorganic particles. The mass concentration of aluminum containing particles transmitted in mainstream smoke was low (.89-.56 ng/cigarette), which was not surprising because aluminum silicates are not volatile. Although the collective masses (ng/cigarette) of aluminum, silicon, and titanium containing particles under 100 nm diameter transported in mainstream smoke were low, an abundance of "ultrafine" particles (particles <100nm or nanoparticles) was observed. Limitations of the particle background equivalent diameter (BED-the smallest detectable particle size (MassHunter 4.5 Software) (1, 2) due to the environmentally ubiquitous silicon background restricted the determination of silica nanoparticles, but silica particles slightly below 200 nm diameter were consistently detected. Aluminum and titanium containing nanoparticles were observed in all cigarette and little cigar samples, with titanium(IV) oxide particle deliveries consistently fewer in number and smaller in diameter than the other two types of particles. The highest concentrations of aluminum-containing particles (as kaolin) were in the nanoparticle range with much lower concentrations extending to the larger particle sizes (>100nm). The number and range of particle sizes determined in mainstream smoke is consistent with pulmonary deposition of aluminum silicates described by other researchers as contributing to the "smokers' inclusions" observed in pulmonary macrophages.