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Literature DB >> 3304292

Isolation of the Candida tropicalis gene for P450 lanosterol demethylase and its expression in Saccharomyces cerevisiae.

C Chen, T G Turi, D Sanglard, J C Loper.

Abstract

We have isolated the gene for cytochrome P450 lanosterol 14 alpha-demethylase (14DM) from the yeast Candida tropicalis. This was accomplished by screening genomic libraries of strain ATCC750 in E. coli using a DNA fragment containing the yeast Saccharomyces cerevisiae 14DM gene. Identity of this gene was confirmed by a) observing a heme binding region common to all P450s after sequencing the 3' portion of the gene, and b) based upon tests of its expression in strains of Saccharomyces cerevisiae.

Entities: Chemical Gene Species

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Year: 1987 PMID： 3304292 DOI： 10.1016/0006-291x(87)90792-3

Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575

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Cited

9 in total

1. Molecular biological characterization of an azole-resistant Candida glabrata isolate.

Authors: P Marichal; H Vanden Bossche; F C Odds; G Nobels; D W Warnock; V Timmerman; C Van Broeckhoven; S Fay; P Mose-Larsen
Journal: Antimicrob Agents Chemother Date: 1997-10 Impact factor: 5.191

Review 2. Clinical, cellular, and molecular factors that contribute to antifungal drug resistance.

Authors: T C White; K A Marr; R A Bowden
Journal: Clin Microbiol Rev Date: 1998-04 Impact factor: 26.132

3. Isolation and molecular characterisation of the gene encoding eburicol 14 alpha-demethylase (cYP51) from Penicillium italicum.

Authors: J G van Nistelrooy; J M van den Brink; J A van Kan; R F van Gorcom; M A de Waard
Journal: Mol Gen Genet Date: 1996-04-10

4. The presence of an R467K amino acid substitution and loss of allelic variation correlate with an azole-resistant lanosterol 14alpha demethylase in Candida albicans.

Authors: T C White
Journal: Antimicrob Agents Chemother Date: 1997-07 Impact factor: 5.191

5. Increased mRNA levels of ERG16, CDR, and MDR1 correlate with increases in azole resistance in Candida albicans isolates from a patient infected with human immunodeficiency virus.

Authors: T C White
Journal: Antimicrob Agents Chemother Date: 1997-07 Impact factor: 5.191

Isolation of the Candida tropicalis gene for P450 lanosterol demethylase and its expression in Saccharomyces cerevisiae.

1. Molecular biological characterization of an azole-resistant Candida glabrata isolate.

Review 2. Clinical, cellular, and molecular factors that contribute to antifungal drug resistance.

3. Isolation and molecular characterisation of the gene encoding eburicol 14 alpha-demethylase (cYP51) from Penicillium italicum.

4. The presence of an R467K amino acid substitution and loss of allelic variation correlate with an azole-resistant lanosterol 14alpha demethylase in Candida albicans.

5. Increased mRNA levels of ERG16, CDR, and MDR1 correlate with increases in azole resistance in Candida albicans isolates from a patient infected with human immunodeficiency virus.

6. Experimental induction of fluconazole resistance in Candida tropicalis ATCC 750.

7. Characterization of an azole-resistant Candida glabrata isolate.

Review 8. Fungal Cytochrome P450s and the P450 Complement (CYPome) of Fusarium graminearum.

Review 9. Cytochrome P450 proteins and potential utilization in biodegradation.