Literature DB >> 3302724

Rational modification of enzyme catalysis by engineering surface charge.

A J Russell, A R Fersht.   

Abstract

Changing the surface charge of subtilisin by site-directed mutagenesis produces enzymes with significantly shifted pH-activity profiles, higher catalytic activities and altered specificities. Insight into water as a dielectric, the role of ions in electrostatic shielding and the field effects on catalysis is obtained and suggests rules for tailoring pH-activity profiles.

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Year:  1987        PMID： 3302724     DOI： 10.1038/328496a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  49 in total

1.  Inhibitor binding induces active site stabilization of the HCV NS3 protein serine protease domain.

Authors:  G Barbato; D O Cicero; F Cordier; F Narjes; B Gerlach; S Sambucini; S Grzesiek; V G Matassa; R De Francesco; R Bazzo
Journal:  EMBO J       Date:  2000-03-15       Impact factor: 11.598

Review 2.  Stability of protein pharmaceuticals.

Authors:  M C Manning; K Patel; R T Borchardt
Journal:  Pharm Res       Date:  1989-11       Impact factor: 4.200

3.  Improving the activity and stability of GL-7-ACA acylase CA130 by site-directed mutagenesis.

Authors:  Wei Zhang; Yuan Liu; Huabao Zheng; Sheng Yang; Weihong Jiang
Journal:  Appl Environ Microbiol       Date:  2005-09       Impact factor: 4.792

4.  Redesigning protein pKa values.

Authors:  Barbara Mary Tynan-Connolly; Jens Erik Nielsen
Journal:  Protein Sci       Date:  2006-12-22       Impact factor: 6.725

5.  Electrostatic interactions modulate the conformation of collagen I.

Authors:  Uwe Freudenberg; Sven H Behrens; Petra B Welzel; Martin Müller; Milauscha Grimmer; Katrin Salchert; Tilman Taeger; Kati Schmidt; Wolfgang Pompe; Carsten Werner
Journal:  Biophys J       Date:  2007-01-05       Impact factor: 4.033

6.  Competitive binding interaction between Zn2+ and saxitoxin in cardiac Na+ channels. Evidence for a sulfhydryl group in the Zn2+/saxitoxin binding site.

Authors:  L Schild; E Moczydlowski
Journal:  Biophys J       Date:  1991-03       Impact factor: 4.033

7.  Nucleotide sequences of genes encoding the type II chloramphenicol acetyltransferases of Escherichia coli and Haemophilus influenzae, which are sensitive to inhibition by thiol-reactive reagents.

Authors:  I A Murray; J V Martinez-Suarez; T J Close; W V Shaw
Journal:  Biochem J       Date:  1990-12-01       Impact factor: 3.857

8.  Crystal structure of a superantigen bound to MHC class II displays zinc and peptide dependence.

Authors:  K Petersson; M Håkansson; H Nilsson; G Forsberg; L A Svensson; A Liljas; B Walse
Journal:  EMBO J       Date:  2001-07-02       Impact factor: 11.598

9.  Coevolutionary patterns in cytochrome c oxidase subunit I depend on structural and functional context.

Authors:  Zhengyuan O Wang; David D Pollock
Journal:  J Mol Evol       Date:  2007-11       Impact factor: 2.395

10.  Tautomerism, acid-base equilibria, and H-bonding of the six histidines in subtilisin BPN' by NMR.

Authors:  Regina M Day; Craig J Thalhauser; James L Sudmeier; Matthew P Vincent; Ekaterina V Torchilin; David G Sanford; Christopher W Bachovchin; William W Bachovchin
Journal:  Protein Sci       Date:  2003-04       Impact factor: 6.725
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