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Literature DB >> 3302673

Homologous recombination between single-stranded DNA and chromosomal genes in Saccharomyces cerevisiae.

Abstract

Transformation of Saccharomyces cerevisiae strains was examined by using the URA3 and TRP1 genes cloned into M13 vectors in the absence of sequences capable of promoting autonomous replication. These constructs transform S. cerevisiae cells to prototrophy by homologous recombination with the resident mutant gene. Single-stranded DNA was found to transform S. cerevisiae cells at efficiencies greater than that of double-stranded DNA. No conversion of single-stranded transforming DNA into duplex forms could be detected during the transformation process, and we conclude that single-stranded DNA may participate directly in recombination with chromosomal sequences. Transformation with single-stranded DNA gave rise to both gene conversion and reciprocal exchange events. Cotransformation with competing heterologous single-stranded DNA specifically inhibited transformation by single-stranded DNA, suggesting that one of the components in the transformation-recombination process has a preferential affinity for single-stranded DNA.

Entities: Gene Species

Mesh：

Substances：

Year: 1987 PMID： 3302673 PMCID： PMC365363 DOI： 10.1128/mcb.7.7.2329-2334.1987

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

21 in total

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Authors: S Fogel; R K Mortimer
Journal: Annu Rev Genet Date: 1971 Impact factor: 16.830

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Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

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Authors: H C Birnboim; J Doly
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

Review 4. Molecular aspects of genetic exchange and gene conversion.

Authors: R Holliday
Journal: Genetics Date: 1974-09 Impact factor: 4.562

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Authors: B A Kunz; R H Haynes
Journal: Annu Rev Genet Date: 1981 Impact factor: 16.830

6. Transformation of yeast.

Authors: A Hinnen; J B Hicks; G R Fink
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

7. Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.

Authors: G Tschumper; J Carbon
Journal: Gene Date: 1980-07 Impact factor: 3.688

8. Yeast transformation: a model system for the study of recombination.

Authors: T L Orr-Weaver; J W Szostak; R J Rothstein
Journal: Proc Natl Acad Sci U S A Date: 1981-10 Impact factor: 11.205

9. recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange.

Authors: M M Cox; I R Lehman
Journal: Proc Natl Acad Sci U S A Date: 1981-06 Impact factor: 11.205

10. Homologous pairing of DNA molecules promoted by a protein from Ustilago.

Authors: E Kmiec; W K Holloman
Journal: Cell Date: 1982-06 Impact factor: 41.582

25 in total

1. Transcription affects formation and processing of intermediates in oligonucleotide-mediated gene alteration.

Authors: Olga Igoucheva; Vitali Alexeev; Melissa Pryce; Kyonggeun Yoon
Journal: Nucleic Acids Res Date: 2003-05-15 Impact factor: 16.971

2. Mechanisms of intermolecular homologous recombination in plants as studied with single- and double-stranded DNA molecules.

Authors: M J de Groot; R Offringa; M P Does; P J Hooykaas; P J van den Elzen
Journal: Nucleic Acids Res Date: 1992-06-11 Impact factor: 16.971

Homologous recombination between single-stranded DNA and chromosomal genes in Saccharomyces cerevisiae.

Review 1. Recombination in yeast.

2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

3. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Review 4. Molecular aspects of genetic exchange and gene conversion.

Review 5. Phenomenology and genetic control of mitotic recombination in yeast.

6. Transformation of yeast.

7. Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.

8. Yeast transformation: a model system for the study of recombination.

9. recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange.

10. Homologous pairing of DNA molecules promoted by a protein from Ustilago.

1. Transcription affects formation and processing of intermediates in oligonucleotide-mediated gene alteration.

2. Mechanisms of intermolecular homologous recombination in plants as studied with single- and double-stranded DNA molecules.

3. A DNA double chain break stimulates triparental recombination in Saccharomyces cerevisiae.

4. Transformation of yeast with synthetic oligonucleotides.

5. Mismatch-stimulated plasmid integration in yeast.

6. Short DNA fragments induce site specific recombination in mammalian cells.

7. Single-stranded DNA as a recombination substrate in plants as assessed by stable and transient recombination assays.

8. Transformation by integration in Podospora anserina. III. Replacement of a chromosome segment by a two-step process.

9. Efficient UV stimulation of yeast integrative transformation requires damage on both plasmid strands.

10. Targeted recombination with single-stranded DNA vectors in mammalian cells.