More Severe Erosive Phenotype Despite Lower Circulating Autoantibody Levels in Dipeptidyl Peptidase-4 Inhibitor (DPP4i)-Associated Bullous Pemphigoid: A Retrospective Cohort Study.

BACKGROUND: The clinical and immunological profile of patients with dipeptidyl peptidase-4 inhibitor (DPP4i)-associated bullous pemphigoid (BP) is inconsistent in the current literature.
OBJECTIVES: The aims were to investigate the clinical and immunological features of patients with DPP4i-associated BP and to examine whether there are intraclass differences between different DPP4i agents.
METHODS: A retrospective cohort study was conducted, including all consecutive patients diagnosed with BP throughout the years 2009-2019 in a tertiary referral center.
RESULTS: The study encompassed 273 patients with BP (mean age at diagnosis 79.1 ± 9.9 years), of whom 24 (8.8%) were associated with DPP4i. Sitagliptin was the prescribed agent for 17 patients (70.8%), and vildagliptin was prescribed in seven patients (29.2%). Relative to other patients with BP, patients with DPP4i-associated BP had more prominent truncal involvement (95.8% vs. 73.9%; P = 0.017), greater erosion/blister Bullous Pemphigoid Disease Area Index (BPDAI) subscore (29.8 ± 17.4 vs. 20.6 ± 14.4; P = 0.018), and lower levels of anti-BP180 NC16A (279.2 ± 346.1 vs. 572.2 ± 1352.0 U/ml; P = 0.009) and anti-BP230 (25.5 ± 47.8 vs. 128.6 ± 302.9 U/ml; P = 0.009) antibodies. Relative to patients with sitagliptin-associated BP, those with vildagliptin-associated BP had a lower seropositivity rate (57.1% vs. 94.1%, P = 0.031) and lower levels (96.7 ± 139.0 vs. 354.5 ± 376.5; P = 0.023) of anti-BP180 NC16A antibodies, and tended to present with higher erosion/blister BPDAI subscore (36.3 ± 9.6 vs. 25.8 ± 19.7; P = 0.095).
CONCLUSIONS: DPP4i-associated BP is characterized by a more severe blistering and erosive presentation despite lower levels of typically pathogenic antibodies.

Key Points

Introduction

Bullous pemphigoid (BP) is the most prevalent subepidermal autoimmune bullous disease in Western Europe and Northern America [1]. During the last 2 decades, a 1.9- to 4.3-fold rise in the incidence of BP was noted in France, Germany, and Israel, an epidemiological finding that has been attributed to multiple factors, including growing exposure to culprit medications [2]. Several lines of evidence suggested that dipeptidyl peptidase-4 inhibitors (DPP4i), or gliptins, a class of oral hypoglycemic drugs recently utilized to manage type 2 diabetes mellitus, may induce or aggravate BP. Data from a wide array of controlled epidemiological studies accumulated throughout the past few years to indicate that treatment with DPP4i is associated with an increased risk of developing BP [3-10].

Although exposure to DPP4i appears to be a risk factor of BP [11], it is yet to be delineated whether patients with DPP4i-associated BP possess a unique morphological, immunological, genetic, or histological profile distinguishing them from patients with classical BP [12-14]. While some studies observed distinct characteristics of this group of patients [6, 15–18], others refuted the presence of such characteristics [9, 19–21].

The primary aim of the current study was to evaluate the clinical and immunological characteristics of patients with DPP4i-associated BP relative to those of diabetic and non-diabetic patients with non-DPP4i-associated classical BP. The secondary aim was to assess the existence of intraclass differences between different DPP4i agents in terms of the clinical and immunological features of the associated BP.

Methods

Study Population and Definition of Cases

We performed a retrospective study comprising all patients diagnosed with BP between January 1st, 2009, and February 28th, 2020, at the Department of Dermatology, University of Lűbeck, Lűbeck, Germany. The current study was approved by the institutional ethical committee (20-110A).

The diagnosis of BP was based on the following criteria: (1) suggestive clinical picture; (2) linear deposits of IgG and⁄or C3 along the dermal–epidermal junction by direct immunofluorescence (IF) microscopy of a perilesional skin biopsy; and (3) the detection of circulating autoantibodies binding to the epidermal side of 1 ml NaCl-split normal human skin by indirect IF microscopy, and⁄or the presence of circulating IgG autoantibodies against BP180 and⁄or BP230, as identified by BP180 NC16A/BP230 enzyme-linked immunosorbent assay (ELISA) [22].

Definition of Covariates

The severity of disease was evaluated based on the Bullous Pemphigoid Disease Area Index (BPDAI) [23]. This score had been documented including its following subcomponents (erosion/blister activity, urticaria/erythema activity, damage, and pruritus). The non-inflammatory phenotype was defined in patients whose BPDAI urticaria/erythema score was zero [17].

The levels of anti-BP180 NC16A and anti-BP230 antibodies were measured using commercially available ELISA assays (Euroimmun, Lűbeck, Germany). The cutoff values proposed by the manufacturer (i.e., 20 U/ml) were adopted for the definition of positivity.

Statistical Analysis

All continuous parameters were expressed as mean values (standard deviation [SD]). Percentages of different patient groups were compared using a Chi-square test. Normally distributed data were analyzed using the student t test. Data found to be non-normally distributed were analyzed using the Mann–Whitney U test for independent subgroups and the Wilcoxon test for dependent subgroups. SPSS software, version 25 (SPSS, Armonk, NY: IBM Corp) was utilized to conduct all statistical analyses.

Results

Study Population

The study cohort included 273 patients with BP, of whom 119 (43.6%) were males, and 154 (56.4%) females. The mean age (SD) at diagnosis was 79.1 (9.9) years, and the median age was 80.4 (range 49.6–98.2) years.

Overall, 75 patients (27.5%) were diagnosed with type 2 diabetes mellitus at the onset of BP. Twenty-four patients (8.8%) developed BP while being treated with DPP4i agents. Among those, the most frequently prescribed DPP4i was sitagliptin (n = 17; 70.8%), followed by vildagliptin (n = 7; 29.2%). No BP patients received another DPP4i agent.

Characterization of Patients with DPP4i-Associated BP

The primary endpoint of the current study was to address the differences between patients receiving DPP4i at the diagnosis of BP (n = 24) relative to BP patients without DPP4i exposure (n = 249). No significant differences were observed between the subgroups with respect to the age of presentation and sex distribution (Table 1).

Table 1

Demographic, clinical, and immunological characteristics of patients with DPP4i-associated BP compared with BP patients not taking DPP4i

	DPP4i-associated BP (n = 24)	Non-DPP4i-associated BP (n = 249)	P value
Age at diagnosis; years
Mean (SD)	77.48 (6.4)	79.20 (10.2)	0.247
Median (range)	77.74 (61.5–89.7)	80.65 (49.6–98.2)
Sex, n (%)
Male	11 (45.8)	108 (43.4)	0.813
Female	13 (54.2)	141 (56.6)
Distribution of bullous lesions, n (%)
Limbs	18 (75.0)	213 (85.5)	0.175
Trunk	23 (95.8)	184 (73.9)	0.017
Hands/feet	11 (45.8)	100 (40.2)	0.595
Head and neck	7 (29.2)	68 (27.3)	0.842
Mucosal involvement	3 (12.5)	28 (11.2)	0.848
Non-inflammatory phenotype, n (%)*	1 (6.3)	16 (13.8)	0.403
Mean BPDAI severity score (SD)*
Erosion/blister activity	29.8 (17.4)	20.6 (14.4)	0.018
Urticaria/erythema activity	12.5 (6.8)	12.5 (16.0)	0.999
Damage score	2.2 (3.6)	2.1 (3.1)	0.896
Pruritus score	20.3 (10.1)	19.1 (8.9)	0.627
Anti-BP180 NC16A ELISA**
Seropositivity, n (%)	20 (83.3)	201 (82.7)	0.940
ELISA value, mean (SD); U/ml	279.2 (346.1)	572.2 (1352.0)	0.009
Anti-BP230 ELISA***
Seropositivity, n (%)	3 (30.0)	38 (52.1)	0.193
ELISA value, mean (SD); U/ml	25.5 (47.8)	128.6 (302.9)	0.009

Significant values are shown in bold

Anti-BP180 NC16A and anti-BP230 antibodies levels were measured via ELISA; cutoff: 20.0 U/ml

BP bullous pemphigoid, BPDAI Bullous Pemphigoid Disease Area Index, DPP4i dipeptidyl peptidase-4 inhibitor(s), ELISA enzyme-linked immunosorbent assay, n number, SD standard deviation

*Was calculated for 16 patients with DPP4i-associated BP and 116 patients with non-DPP4i-associated BP

**Was performed in all patients with DPP4i-associated BP and in 243 patients with non-DPP4i-associated BP

***Was performed in 10 patients with DPP4i-associated BP and in 73 patients with non-DPP4i-associated BP

Regarding the anatomical distribution of bullous lesions, patients with DPP4i-associated BP had greater truncal involvement (95.8% vs. 73.9%; P = 0.017). No significant differences were observed between the two subgroups regarding the involvement of other body sites or the prevalence of the non-inflammatory phenotype of BP. Patients were additionally compared in terms of their BPDAI scores. Patients with DPP4i-associated BP were found to have a higher blister/erosion BPDAI mean (SD) score (29.8 [17.4] vs. 20.6 [14.4], respectively; P = 0.018; Fig. 1a). The remaining components of the BPDAI score [namely, urticaria/erythema (Fig. 1b), damage, and pruritus] were comparable between the two subgroups (Table 1).

Fig. 1

Values of erosion/blister (a) and urticaria/erythema (b) BPDAI scores among patients with DPP4i-associated BP vs. non-DPP4i-associated BP. BP bullous pemphigoid, BPDAI Bullous Pemphigoid Disease Area Index, DPP4i dipeptidyl peptidase-4 inhibitor

Overall, 267 (97.8%) of patients were tested for the presence of circulating anti-BP180 NC16A antibodies. While the detection rate of anti-BP180 NC16A antibodies was comparable between the two subgroups, patients with DPP4i-associated BP had significantly lower mean (SD) levels of these antibodies (279.2 [346.1] vs. 572.2 [1352.0] U/ml, respectively; P = 0.009; Fig. 2). Additionally, 83 patients (30.4%) were tested for anti-BP230 antibodies. The mean (SD) anti-BP230 levels were also lower among patients with DPP4i-associated BP (25.2 [47.8] vs. 128.6 [302.9] U/ml, respectively; P = 0.009), while the seropositivity of these antibodies tended to be lower in this subgroup (30% vs. 52.1%, respectively; P = 0.193; Table 1).

Fig. 2

Levels of anti-BP180 NC16A antibodies among patients with DPP4i-associated BP vs. non-DPP4i-associated BP. Rules represent mean values. BP bullous pemphigoid, DPP4i dipeptidyl peptidase-4 inhibitor

To exclude any confounding factor through the additional diagnosis of diabetes mellitus, we next studied the difference between diabetic patients with DPP4i-associated BP (n = 24) relative to diabetic patients with non-DPP4i-associated BP (n = 51). While no morphological discrepancies appeared between the two subgroups, the former group again had lower levels of circulating anti-BP180 NC16A antibodies (279.2 [346.1] vs. 696.2 [1340.1] U/ml; P = 0.045), lower positivity rate (30.0% vs. 81.3%; P = 0.010) as well as lower levels (25.5 [47.8] vs. 211.4 [330.3] U/ml; P = 0.042) of anti-BP230 antibodies. The mean blister/erosion BPDAI score tended to be higher among patients with DPP4i-associated BP, although it lacked statistical significance (Table 2).

Table 2

Demographic, clinical, and immunological characteristics of patients with DPP4i-associated BP compared with diabetic patients not taking DPP4i

	DPP4i-associated BP (n = 24)	Non-DPP4i-associated diabetic BP (n = 51)	P value
Age at diagnosis; years
Mean (SD)	77.5 (6.4)	79.7 (8.8)	0.225
Sex, n (%)
Male	11 (45.8)	29 (56.9)	0.372
Female	13 (54.2)	22 (43.1)
Distribution of bullous lesions; n (%)
Limbs	18 (75.0)	46 (90.2)	0.175
Trunk	23 (95.8)	38 (74.5)	0.085
Hands/feet	11 (45.8)	21 (41.2)	0.709
Head and neck	7 (29.2)	19 (37.3)	0.495
Mucosal involvement	3 (12.5)	4 (7.8)	0.516
Non-inflammatory phenotype, n (%)*	1 (6.3)	6 (21.4)	0.192
Mean BPDAI severity score (SD)*
Erosions/blister activity	29.8 (17.4)	23.4 (14.9)	0.128
Urticaria/erythema activity	12.5 (6.8)	8.7 (11.7)	0.145
Damage score	2.2 (3.6)	2.4 (3.6)	0.824
Pruritus score	20.3 (10.1)	18.8 (8.4)	0.531
Anti-BP180 NC16A ELISA**
Seropositivity, n (%)	20 (83.3)	43 (87.8)	0. 602
ELISA value, mean (SD); U/ml	279.2 (346.1)	696.2 (1340.1)	0.045
Anti-BP230 ELISA***
Seropositivity, n (%)	3 (30.0)	13 (81.3)	0.010
ELISA value, mean (SD); U/ml	25.5 (47.8)	211.4 (330.3)	0.042

Significant values are shown in bold

Anti-BP180 NC16A and anti-BP230 antibodies levels were measured via ELISA; cutoff: 20.0 U/ml

BP bullous pemphigoid, BPDAI Bullous Pemphigoid Disease Area Index, DPP4i dipeptidyl peptidase-4 inhibitor(s), ELISA enzyme-linked immunosorbent assay, n number, SD standard deviation

*Was calculated for 16 patients with DPP4i-associated BP and for 28 diabetic patients with non-DPP4i-associated BP

**Was performed in all patients with DPP4i-associated BP and in 49 diabetic patients with non-DPP4i-associated BP

***Was performed in 10 patients with DPP4i-associated BP and in 16 diabetic patients with non-DPP4i-associated BP

To refute differential effect exerted by other anti-diabetic medications, the two subgroups were compared with regard to exposure to non-DPP4i anti-diabetic medications. Out of patients with DPP4i-associated BP, 17 (70.8%) were managed by additional anti-diabetic medications, whereas 38 diabetic patients with non-DPP4i-associated BP (74.5%) had an exposure to these medications (P = 0.737). The two subgroups were comparable with regard to exposure to metformin (45.8% vs. 33.3%, respectively; P = 0.299) and insulin (45.8% vs. 47.1%, respectively; P = 0.917).

Identification of Intraclass Variations

Our secondary endpoint was to evaluate intraclass differences in the clinical and immunological features of BP associated with different DPP4i agents. A comparison was undertaken between patients with BP under the two encountered agents: sitagliptin (n = 17) and vildagliptin (n = 7; Table 3).

Table 3

Demographic, clinical, and immunological characteristics of sitagliptin-associated BP compared with vildagliptin-associated BP patients

Characteristic	Sitagliptin-associated BP (n = 17)	Vildagliptin-associated BP (n = 7)	P value
Age at diagnosis; years
Mean (SD)	78.1 (6.1)	75.9 (7.0)	0.456
Sex, n (%)
Male	9 (52.9)	2 (28.6)	0.288
Female	8 (47.1)	5 (71.4)
Mean BPDAI severity score (SD)*
Erosions/blister activity	25.8 (19.7)	36.3 (9.6)	0.095
Urticaria/erythema activity	11.1 (6.1)	15.5 (7.2)	0.186
Damage score	2.3 (3.6)	2.0 (3.5)	0.853
Anti-BP180 NC16A ELISA
Seropositivity, n (%)	16 (94.1)	4 (57.1)	0.031
ELISA value, mean (SD); U/ml	354.5 (376.5)	96.7 (139.0)	0.023
Anti-BP230 ELISA**
Seropositivity, n (%)	2 (33.3)	1 (25.0)	0.790
ELISA value, mean (SD); U/ml	37.0 (58.7)	8.3 (7.6)	0.368

Significant values are shown in bold

Anti-BP180 NC16A and anti-BP230 antibodies levels were measured via ELISA; cutoff: 20 U/ml

BP bullous pemphigoid, BPDAI Bullous Pemphigoid Disease Area Index, DPP4i dipeptidyl peptidase-4 inhibitor(s), ELISA enzyme-linked immunosorbent assay, n number, SD standard deviation

*Was calculated for 10 patients with sitagliptin-associated BP and for 6 patients with vildagliptin-associated BP

**Was performed in 6 patients with sitagliptin-associated BP and in 4 patients with vildagliptin-associated BP

Patients with sitagliptin-associated BP had a higher seropositivity rate (94.1% vs. 57.1%, P = 0.031) and higher mean (SD) levels (354.5 [376.5] vs. 96.7 [139.0] U/ml; P = 0.023) of anti-BP180 NC16A antibodies. A tendency towards a greater mean (SD) erosion/blister BPDAI score was observed in patients with vildagliptin-associated BP (36.3 [9.6] vs. 25.8 [19.7]), albeit it did not reach the level of statistical significance (P = 0.095; Table 3).

Discussion

Our current study revealed that patients with DPP4i-associated BP are characterized by more predominant truncal involvement, a more severe blistering phenotype (as reflected by erosion/blister BPDAI scores), and significantly lower serum levels of anti-BP180 NC16A and anti-BP230 autoantibodies. Compared to patients with vildagliptin-associated BP, those with sitagliptin-associated BP had a higher positivity rate and higher levels of anti-BP180 NC16A antibodies.

Despite the accumulating data on the association of BP with DPP4i, there remains a debate around the clinical and immunological profile of patients with DPP4i-associated BP. This inconclusiveness stems mainly from the variable findings observed in different studies. Notable differences were mainly observed between studies from Japan attributing distinct features (like non-inflammatory phenotype and targeting atypical epitopes along BP180) to Japanese patients on one side and studies reporting typical features in European patients on the other. Table 4 summarizes the main findings of the studies carried out so far to investigate the different features of patients with DPP4i-associated BP.

Table 4

Summary of the main findings of studies characterizing patients with DPP4i-associated BP

Study	Country	N of patients with DPP4i-associated BP	N of patients with non-DPP4i-associated BP	Morphological features	Immunological features	Histological features	Genetic features	Laboratory features
Izumi et al. [16]	Japan	10	76	Non-inflammatory phenotype (70%); lower urticaria/erythema BPDAI score	Antibodies against full-length BP180 but not against BP180 NC16A (70%); antibodies against BP180 NC16A (30%)	Lower dermal infiltrating eosinophils	–	–
Horikawa et al. [17]	Japan	12	23	Non-inflammatory phenotype (50%); lower urticaria/erythema BPDAI score	Antibodies against full-length BP180 but not against BP180 NC16A (42%); antibodies against BP180 NC16A (58%)	–	–	–
Chijiwa et al. [15]	Japan	9	21	Higher erosion/blister BPDAI in mucosa score	Comparable levels of anti-BP180 NC16A antibodies	Lower dermal infiltrating eosinophils	–	Comparable peripheral eosinophil count
Ujiie et al. [18]	Japan	30 (21 non-inflammatory vs. 9 inflammatory)	–	Non-inflammatory phenotype (70%)	Lower levels of anti-BP180 NC16A antibodies (in the non-inflammatory subgroup)	Lower dermal infiltrating eosinophils (in the non-inflammatory subgroup)	86% of non-inflammatory BP patients had HLA-DQB1*03:01 haplotype	–
Kridin and Bergman [6]	Israel	36	46	Greater mucosal involvement	–	–	–	Lower peripheral eosinophil count
Patsatsi et al. [20]	Greece	47	95	Tendency to higher BPDAI scores (P = 0.063)	Comparable positivity and levels of anti-BP180 NC16A (57.4%) and anti-BP230 (21.3%) antibodies	Comparable dermal infiltrating eosinophils	–	–
Fania et al. [21]	Italy	5	–	Inflammatory phenotype (100%)	IgG antibodies against BP180 NC16A (100%), BP230 (80%), epitopes in the mid portion (50%), and epitopes in C-terminus (75%); IgE antibodies against BP230 (75%)	–	–	–
Plaquevent et al. [9]	France	108	–	40.7% of patients had severe BP (> 10 new blisters/day)	Antibodies against BP180 NC16A (70.7%) and BP230 (37.9%)	–	–	–
Lindgren et al. [19]	Finland	10	17	Non-inflammatory phenotype (40%); less frequently neurological diseases	Antibodies against BP180 NC16A (70.0%); insignificantly higher levels of BP180 NC16A antibodies (P = 0.481)	Comparable dermal infiltrating eosinophils; Comparable expression of DPP4/CD26 in lesional BP skin samples	Comparable frequency of HLA-DQB1*03:01 (44%)	–
Kridin [26]	Israel	58	339	More extensive disease; more cephalic and truncal involvement; male preponderance (60.3%)	–	–	–	Lower peripheral eosinophil count
Current study	Germany	24	249	Higher erosion/blister BPDAI score; more truncal involvement	Antibodies against BP180 NC16A (83.3%); lower levels of anti-BP180 NC16A and anti-BP230 antibodies	–	–	–

BP bullous pemphigoid, BPDAI Bullous Pemphigoid Disease Area Index, DPP4i dipeptidyl peptidase-4 inhibitor(s), n number

Unlike other studies reporting a male predominance among patients with DPP4i-associated BP [3, 6, 8, 24], the sex distribution in our cohort was similar between the two subgroups, in line with two studies from Finland [4] and France [9].

The non-inflammatory phenotype was a prominent morphological feature among Japanese patients with DPP4i-associated BP, where its prevalence ranged between 50 and 70% [16-18]. This finding was not reproduced in studies tracking Caucasian patients, where the non-inflammatory phenotype was less frequent (40% in Finland [19], 0% in Italy [21], and 6.3% in the current study). A clinicopathological correlation was reported in Japanese patients demonstrating that the non-inflammatory phenotype was paralleled by an only scant eosinophilic dermal infiltrate in perilesional skin [15, 16, 18]. A study from Israel lent weight to this finding by detecting lower circulating eosinophil counts among these patients [6]. On the contrary, two European studies refuted the existence of a distinct histological pattern by revealing a comparable dermal eosinophil count in patients with DPP4i-associated BP relative to their non-DPP4I-associated BP counterparts (Table 4) [19, 20]. To shed further light on ethnic variations, a recent American study utilized two large commercial insurance claims databases to follow patients placed on DPP4i and second-generation sulfonylureas. This study revealed an increased risk of BP in the former groups, with White individuals possessing a higher risk than their non-white counterparts [10]. Unlike a previous study attributing a higher frequency of mucosal lesions in patients with DPP4i-associated BP [6, 25], mucosal involvement among our DPP4i-associated patients was as common as among the remaining patients.

The current study suggests that patients with DPP4i-associated BP had a more severe bullous component, as indicated by a higher erosion/blister BPDAI score. This finding is in accordance with an Israeli study evaluating 58 patients with DPP4i-associated BP, who presented with significantly more extensive disease [26]. Additionally, Patsatsi et al. [20] revealed that patients with DPP4i-associated BP had higher total BPDAI scores, with a trend towards significance (41.0 vs. 34.1; P = 0.063). Similarly, Horikawa et al. [17] and Chijiwa et al. [15] demonstrated that the erosion/blister component of the BPDAI score tended to be higher among patients with DPP4i-associated BP, albeit lacking statistical significance. Given the relatively small sample size of these studies, one may assume that they were underpowered to provide differences of statistical significance; still, they reflected a trend towards a more severe phenotype.

Immunologically, we found that patients with DPP4i-associated BP had significantly lower levels of anti-BP180 NC16A antibodies. This finding held significance also when comparing this subgroup to diabetic patients with non-DPP4i-associated BP, thus refuting major confounding conferred by diabetes mellitus itself. While this finding aligns with a previous study by Ujiie et al. [18], it is not consistent with the studies of Patsatsi et al. [20], Chijiwa et al. [15], and Lindgren et al. [19] reporting similar levels of these antibodies in DPP4i-associated BP and non-DPP4i-associated BP. The positivity rate of anti-BP180 NC16A antibodies in the current study was estimated at 83.3% among patients with DPP4i-associated BP. While earlier studies, originating mainly from Japan, disclosed low seropositivity of the aforementioned antibodies ranging between 30 and 58% [16, 17, 20], later European studies have shown higher detection rates, ranging from 70 to 100% [9, 19, 21], a range which our figure falls within (Table 3). In a recent Japanese cross-sectional study examining sera from type 2 diabetes mellitus patients with and without DPP4i exposure, the former group had higher levels of anti-full-length BP180, but not anti-BP180 NC16A and anti-BP230 IgG [27]. The latter finding may lend credibility to our observation regarding the lower levels of anti-BP180 NC16A. In a recent study of 18 Japanese DPP4i-associtaed BP patients targeting the non-NC16A domains of BP180, Mai et al. [28] revealed that the all sera reacted more intensively with the 97-kDa processed extracellular domain of BP180 (LABD97) autoantigen than with full-length BP180. Thus, anti-LABD97 IgG1 represented the major autoantibodies in these DPP4i-associated BP patients.

The detection rate of anti-BP230 antibodies in this study (30%) compares with the figures reported by Patsatsi et al. [20] (21.3%) and Plaquevent et al. [9] (37.9%). The relative serum levels of this autoantibody had been assessed only by Patsatsi et al. [20] and were found to be similar between patients with DPP4i-associated BP relative to those with typical BP. In contrast, we found decreased levels in the current study.

Taken together, in our cohort of 273 BP patients, those with DPP4i-associated BP presented with a more severe erosive disease despite generating lower levels of autoantibodies (both anti-NC16A BP180 and anti-BP230 IgG). This intriguing finding may lead us to postulate that patients with this disease target other epitopes along the BP180 autoantigen, which are not detected by the commercially available ELISA. Small-scale Japanese studies assumed that these patients synthesize autoantibodies against epitopes along the midportion of the extracellular domain of BP180, but not against the NC16A domain [16, 17]. We were not able to examine this hypothesis since our retrospective analysis merely evaluated the presence of IgG autoantibodies against the immunodominant NC16A domain of BP180. Additional immunoblotting was performed in a few of our patients, but did not enable us to draw meaningful conclusions. Alternatively, autoantibodies from DPP4i-associated BP may have other, more pro-inflammatory properties, i.e., a G0 IgG glycosylation pattern that was shown to promote skin inflammation [29].

It is still unknown whether different DPP4i agents trigger BP with different clinical and immunological features. Interestingly, we observed that patients with vildagliptin-associated BP had a lower seropositivity rate of antibodies targeting the immunodominant domain of BP180 and exhibited lower levels of these antibodies. However, despite their decreased production of detectable autoantibodies, these patients tended to present with a more widespread blistering/erosive phenotype as compared with their sitagliptin-associated counterparts. This substantiates the assumption that patients with DPP4i-associated BP, particularly those with vildagliptin-associated BP, either target other yet unknown epitopes, or generate highly pathogenic autoantibodies. Although the highest risk of DPP4i-associated BP was ascribed to vildagliptin [11], patients with sitagliptin-associated BP were more frequently encountered than their vildagliptin-associated counterparts in our study. The latter finding probably reflects the more common usage of sitagliptin in Germany.

The main limitation of the current study is its retrospective design, which enabled the collection of data of immunoassays utilized for the purpose of fulfilling diagnostic criteria, but not for research purposes. However, the routine immunological work-up at our department is very comprehensive, thus facilitating the characterization of the immunological profile of patients with DPP4i-associated BP. Selection bias may have arisen because the study was performed in tertiary-care referral center settings, rendering it susceptible to overlooking mild BP cases managed in outpatient community settings, Although the general study population was relatively high, some subgroup analyses were based on low numbers of patients.

The current study sheds light on a topic with inconclusive findings reported in the current literature so far. Moreover, it provides novel insights regarding intraclass variations between different DPP4i agents. The routinely collected clinical and immunoserological variables enabled relatively broad profiling of the patients.

Conclusion

In conclusion, we show that patients developing BP under DPP4i treatment are characterized by a more severe erosive phenotype and by more prominent truncal involvement. Immunologically, these patients have lower levels of anti-BP180 NC16A and anti-BP230 autoantibodies. Patients with vildagliptin-associated BP have an even lower prevalence of these specific autoantibodies, decreased levels of anti-NC16A BP180 IgG, and tended to show a more severe erosive phenotype. Given the lack of correlation between clinical disease severity and the routinely examined autoantibody levels, our findings indicate that patients with DPP4i-associated BP produce autoantibodies against other epitopes than those with non-DPP4i-associated BP or, alternatively, that DPP4i lead to the production of more pro-inflammatory autoantibodies.

Patients with dipeptidyl peptidase-4 inhibitor–associated bullous pemphigoid (BP) demonstrate lower levels of anti-BP180 NC16A autoantibodies and a more severe erosive phenotype.

Patients with vildagliptin-associated BP had a lower seropositivity rate and lower levels of anti-BP180 NC16A antibodies compared with patients with sitagliptin-associated BP.

Given the inverse association between autoantibody levels and disease severity, it may be hypothesized that these patients target other epitopes or, alternatively, have a lower threshold to induce autoantibody-induced skin pathology in BP.