Influence of nutrient status on the biohydrogen and lipid productivity in Parachlorella kessleri: a biorefinery approach.

The commercial reality of microalgal biotechnology for the production of individual bioactives is constrained by the high cost of production and requires a biorefinery approach. In this investigation, we examined the influence of different nutrient deprivation (nitrogen (N), phosphorus (P), sulphur (S) and manganese (Mn)) on growth, chlorophyll a (Chl a), biohydrogen (H2) and fatty acid profiles in Parachlorella kessleri EMCCN 3073 under both aerobic and anaerobic conditions. Anaerobic conditions combined with the nutrient deprivation resulted in cell division blockage, reduction in Chl a and remarkable changes in pH, whereas a significant increase in the H2 production was observed after 24 h. The highest cumulative H2 productivity was observed in N-deficient medium (300 μL/L, day 9) followed by Mn-deficient medium (250 μL/L, day 7). The highest H2 production rate (3.37 μL/L/h) was achieved by Mn-deficient medium after 24 h. In terms of fatty acid composition, P. kessleri exhibited a differential response to different nutrient stresses. Under aerobic conditions, N-deficient media resulted in the highest lipid content (119% compared to control, day 7), whereas earlier lipid induction at (1-3 days) was observed with Mn- and S-deficient media with 18-91% and 25-34% increase, respectively, compared with the replete control. Meanwhile, higher lipid content was observed under anaerobic conditions combined with Mn-, N-, P- and S-deprived media (day 1) with 20%, 13%, 8% and 7% increases respectively compared with the control. This investigation, for the first time clearly, highlights the potential of P. kessleri as a sustainable biorefinery platform, for H2 and fatty acid bio-production under anaerobic conditions. KEY POINTS: • Parachlorella kessleri could provide a future sustainable biorefinery platform. • Nutrient-deprived anaerobic conditions blocked cell growth but differentially induced H2 production. • Nutrient status, under both aerobic/anaerobic conditions, alters lipids and fatty acids profile of P. kessleri. • Nutrient-deprived (N- and Mn-) anaerobic conditions: future biorefinery platform.