Jelena M Živković1,2, Sanja T Stojanović3,4, Marija Đ Vukelić-Nikolić3,4, Milena B Radenković4, Jelena G Najdanović3,4, Milan Ćirić5, Stevo J Najman3,4. 1. Department of Biology and Human Genetics, Faculty of Medicine, University of Niš, Niš, 18108, Serbia. jelena.zivkovic.biologija@medfak.ni.ac.rs. 2. Department for Cell and Tissue Engineering, Scientific Research Center for Biomedicine, Faculty of Medicine, University of Niš, Niš, 18108, Serbia. jelena.zivkovic.biologija@medfak.ni.ac.rs. 3. Department of Biology and Human Genetics, Faculty of Medicine, University of Niš, Niš, 18108, Serbia. 4. Department for Cell and Tissue Engineering, Scientific Research Center for Biomedicine, Faculty of Medicine, University of Niš, Niš, 18108, Serbia. 5. Institute of Physiology, Faculty of Medicine, University of Niš, Niš, 18108, Serbia.
Abstract
PURPOSE: Given the great potential of macrophages in the processes of tissue repair and regeneration, the aim of our study was to examine the contribution that macrophages will have in osteogenic process when combined and implanted with blood clot (BC) and mineral bone substitute (MBS) in mice subcutaneous implantation model. METHODS: Three types of implants were constructed and implanted subcutaneously into BALB/c mice: (1) RMBM implants (made of resident tissue macrophages, BC and MBS), (2) BM implants (made of BC and MBS), and (3) M implants (made of MBS only) where the last two served as control implants. One, two, four and eight weeks after implantation implants were explanted, and histochemical, immunohistochemical, and histomorphometric analyses were performed. RESULTS: Increased vascularization, particularly pronounced two and four weeks after implantation and pronounced tissue infiltration in eight week term in RMBM implants compared with both other types, likewise the presence of osteoblast-like cells, osteoid-like structures, and more prominent osteopontin and osteocalcin immunoexpression in RMBM implants indicated more pronounced osteogenic process within them. CONCLUSION: Our results suggest that macrophages deserve to be considered as a cell component when constructing implants in bone regenerative medicine strategies to improve bone fracture healing process.
PURPOSE: Given the great potential of macrophages in the processes of tissue repair and regeneration, the aim of our study was to examine the contribution that macrophages will have in osteogenic process when combined and implanted with blood clot (BC) and mineral bone substitute (MBS) in mice subcutaneous implantation model. METHODS: Three types of implants were constructed and implanted subcutaneously into BALB/c mice: (1) RMBM implants (made of resident tissue macrophages, BC and MBS), (2) BM implants (made of BC and MBS), and (3) M implants (made of MBS only) where the last two served as control implants. One, two, four and eight weeks after implantation implants were explanted, and histochemical, immunohistochemical, and histomorphometric analyses were performed. RESULTS: Increased vascularization, particularly pronounced two and four weeks after implantation and pronounced tissue infiltration in eight week term in RMBM implants compared with both other types, likewise the presence of osteoblast-like cells, osteoid-like structures, and more prominent osteopontin and osteocalcin immunoexpression in RMBM implants indicated more pronounced osteogenic process within them. CONCLUSION: Our results suggest that macrophages deserve to be considered as a cell component when constructing implants in bone regenerative medicine strategies to improve bone fracture healing process.
Entities:
Keywords:
Blood clot; Ectopic osteogenesis; Implant; Macrophages; Mineral bone substitute; Subcutaneous implantation
Authors: Vladimir J Cvetković; Jelena G Najdanović; Marija Đ Vukelić-Nikolić; Sanja Stojanović; Stevo J Najman Journal: Int Orthop Date: 2015-08-01 Impact factor: 3.075
Authors: Katharina Schmidt-Bleek; Hanna Schell; Norma Schulz; Paula Hoff; Carsten Perka; Frank Buttgereit; Hans-Dieter Volk; Jasmin Lienau; Georg N Duda Journal: Cell Tissue Res Date: 2011-07-26 Impact factor: 5.249