Literature DB >> 330202

Establishment of serum based essentiall free of proteolytic activity for the culture of mouse pancreatic islets.

M Kedinger, A Moody, J F Launay, K Haffen.   

Abstract

The present study demonstrates that a) serum based culture medium degrades 125I inhibits its proteolytic activity leading to the recovery of more insulin secreted by islets cultured in the presence of high glucose concentration alone or with glucagon; c) aprotinin also favoured the accumulation of secreted insulin by protecting the hormone from a residual degradative capacity of the hear treated serum.

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Year:  1977        PMID: 330202     DOI: 10.1007/bf01951312

Source DB:  PubMed          Journal:  Experientia        ISSN: 0014-4754


  3 in total

1.  Metabolic characteristics of isolated pancreatic islets in tissue culture.

Authors:  A Andersson; C Hellerström
Journal:  Diabetes       Date:  1972       Impact factor: 9.461

2.  Effects of glucose on the ultrastructure and insulin biosynthesis of isolated mouse pancreatic islets maintained in tissue culture.

Authors:  A Andersson; J Westman; C Hellerström
Journal:  Diabetologia       Date:  1974-12       Impact factor: 10.122

3.  Method for the isolation of intact islets of Langerhans from the rat pancreas.

Authors:  P E Lacy; M Kostianovsky
Journal:  Diabetes       Date:  1967-01       Impact factor: 9.461

  3 in total
  5 in total

1.  Isolated mouse pancreatic islets in culture: effects of serum and different culture media on the insulin production of the islets.

Authors:  A Andersson
Journal:  Diabetologia       Date:  1978-06       Impact factor: 10.122

2.  Glucagon production by cultured pancreatic islets: effects of different culture conditions and media.

Authors:  A Andersson; U Eriksson; C G Ostenson
Journal:  In Vitro       Date:  1981-05

3.  Preservation of beta cell function in adult human pancreatic islets for several months in vitro.

Authors:  J H Nielsen; J Brunstedt; A Andersson; C Frimodt-Møller
Journal:  Diabetologia       Date:  1979-02       Impact factor: 10.122

4.  Studies on insulin secretion by monolayer cultures of normal and tumorous human pancreatic cells. Effects of glucose, somatostatin and SMS 201-995.

Authors:  R Oosterom; T Verleun; P Uitterlinden; H A Bruining; G J Bruining; L Verschoor; S W Lamberts
Journal:  J Endocrinol Invest       Date:  1987-12       Impact factor: 4.256

5.  A new mutation (db3J) at the diabetes locus in strain 129/J mice. II. Studies of pancreatic alpha cell function in culture.

Authors:  E H Leiter; I Strack; A B Eisenstein
Journal:  Diabetologia       Date:  1980-07       Impact factor: 10.122

  5 in total

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