Literature DB >> 3301806

Activation of the xylDLEGF promoter of the TOL toluene-xylene degradation pathway by overproduction of the xylS regulatory gene product.

R A Spooner, M Bagdasarian, F C Franklin.   

Abstract

The xylS regulatory gene of the Pseudomonas putida TOL plasmid (pWWO) has been cloned under the transcriptional control of the Escherichia coli tac promoter in a broad-host-range controlled-expression vector. Induction with isopropylthiogalactoside allowed overproduction and characterization of the xylS product by specific interaction with the TOL meta-cleavage pathway operator-promoter region (OP2) in vivo in E. coli. Examination of plasmid-specified polypeptides in E. coli maxicells led to identification of the xylS product as a 36-kilodalton polypeptide. The operator sequences required for xylS interactions lay upstream of the OP2 transcriptional start, and the xylS gene product recognized this region even in the absence of known coinducers.

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Year:  1987        PMID： 3301806      PMCID： PMC212435          DOI： 10.1128/jb.169.8.3581-3586.1987

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  23 in total

1.  Molecular cloning of gene xylS of the TOL plasmid: evidence for positive regulation of the xylDEGF operon by xylS.

Authors:  S Inouye; A Nakazawa; T Nakazawa
Journal:  J Bacteriol       Date:  1981-11       Impact factor: 3.490

2.  Physical and functional mapping of RP4-TOL plasmid recombinants: analysis of insertion and deletion mutants.

Authors:  T Nakazawa; S Inouye; A Nakazawa
Journal:  J Bacteriol       Date:  1980-10       Impact factor: 3.490

3.  Construction of a partial diploid for the degradative pathway encoded by the TOL plasmid (pWWO) from Pseudomonas putida mt-2: evidence for the positive nature of the regulation by the xyIR gene.

Authors:  F C Franklin; P A Williams
Journal:  Mol Gen Genet       Date:  1980-01

Review 4.  E. coli RNA polymerase interacts homologously with two different promoters.

Authors:  U Siebenlist; R B Simpson; W Gilbert
Journal:  Cell       Date:  1980-06       Impact factor: 41.582

5.  Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway.

Authors:  F C Franklin; M Bagdasarian; M M Bagdasarian; K N Timmis
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

6.  Metabolism of toluene and xylenes by Pseudomonas (putida (arvilla) mt-2: evidence for a new function of the TOL plasmid.

Authors:  M J Worsey; P A Williams
Journal:  J Bacteriol       Date:  1975-10       Impact factor: 3.490

7.  Regulation of the degradative pathway enzymes coded for by the TOL plasmid (pWWO) from Pseudomonas putida mt-2.

Authors:  M J Worsey; F C Franklin; P A Williams
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

8.  A system for shotgun DNA sequencing.

Authors:  J Messing; R Crea; P H Seeburg
Journal:  Nucleic Acids Res       Date:  1981-01-24       Impact factor: 16.971

9.  Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector.

Authors:  J P Fürste; W Pansegrau; R Frank; H Blöcker; P Scholz; M Bagdasarian; E Lanka
Journal:  Gene       Date:  1986       Impact factor: 3.688

10.  Catabolism of pseudocumene and 3-ethyltoluene by Pseudomonas putida (arvilla) mt-2: evidence for new functions of the TOL (pWWO) plasmid.

Authors:  D A Kunz; P J Chapman
Journal:  J Bacteriol       Date:  1981-04       Impact factor: 3.490
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  13 in total

1.  Functional domains of the TOL plasmid transcription factor XylS.

Authors:  N Kaldalu; U Toots; V de Lorenzo; M Ustav
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

Review 2.  Bacterial transcriptional regulators for degradation pathways of aromatic compounds.

Authors:  David Tropel; Jan Roelof van der Meer
Journal:  Microbiol Mol Biol Rev       Date:  2004-09       Impact factor: 11.056

Review 3.  The TOL (pWW0) catabolic plasmid.

Authors:  R S Burlage; S W Hooper; G S Sayler
Journal:  Appl Environ Microbiol       Date:  1989-06       Impact factor: 4.792

Review 4.  Arac/XylS family of transcriptional regulators.

Authors:  M T Gallegos; R Schleif; A Bairoch; K Hofmann; J L Ramos
Journal:  Microbiol Mol Biol Rev       Date:  1997-12       Impact factor: 11.056

5.  Recruitment and expression of toluene/trichloroethylene biodegradation genes in bacteria native to deep-subsurface sediments.

Authors:  M F Romine; F J Brockman
Journal:  Appl Environ Microbiol       Date:  1996-07       Impact factor: 4.792

6.  Cloning and expression of the benzoate dioxygenase genes from Rhodococcus sp. strain 19070.

Authors:  S Haddad; D M Eby; E L Neidle
Journal:  Appl Environ Microbiol       Date:  2001-06       Impact factor: 4.792

7.  Growth-phase-dependent expression of the Pseudomonas putida TOL plasmid pWW0 catabolic genes.

Authors:  N Hugouvieux-Cotte-Pattat; T Köhler; M Rekik; S Harayama
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

8.  Isolation and characterization of a new plasmid from a Flavobacterium sp. which carries the genes for degradation of 2,4-dichlorophenoxyacetate.

Authors:  G R Chaudhry; G H Huang
Journal:  J Bacteriol       Date:  1988-09       Impact factor: 3.490

9.  Genetic evidence that the XylS regulator of the Pseudomonas TOL meta operon controls the Pm promoter through weak DNA-protein interactions.

Authors:  B Kessler; M Herrero; K N Timmis; V de Lorenzo
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

10.  Location and organization of the dimethylphenol catabolic genes of Pseudomonas CF600.

Authors:  M Bartilson; I Nordlund; V Shingler
Journal:  Mol Gen Genet       Date:  1990-01
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