Literature DB >> 3301770

Purification and characterization of cloned isopenicillin N synthetase.

J E Baldwin, S J Killin, A J Pratt, J D Sutherland, N J Turner, M J Crabbe, E P Abraham, A C Willis.   

Abstract

Isopenicillin N synthetase (IPS) cloned from Cephalosporium acremonium has been isolated from transformed Escherichia coli and purified to homogeneity. The resulting, abundant, recombinant protein, whilst undergoing slightly different N-terminal processing to that observed for the fungally-derived protein, has identical kinetics for the conversion of LLD-aminoadipoyl-cysteinyl-valine to isopenicillin N. Recombinant IPS converts analogue substrates into unusual beta-lactam antibiotics in exactly the same way as the fungal protein.

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Year:  1987        PMID： 3301770     DOI： 10.7164/antibiotics.40.652

Source DB:  PubMed          Journal:  J Antibiot (Tokyo)        ISSN： 0021-8820            Impact factor:   2.649


  9 in total

Review 1.  Molecular biology of penicillin and cephalosporin biosynthesis.

Authors:  S W Queener
Journal:  Antimicrob Agents Chemother       Date:  1990-06       Impact factor: 5.191

2.  Biochemical studies on the activity of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Streptomyces clavuligerus.

Authors:  J Zhang; S Wolfe; A L Demain
Journal:  Biochem J       Date:  1992-05-01       Impact factor: 3.857

3.  Purification and characterization of recombinant Streptomyces clavuligerus isopenicillin N synthase produced in Escherichia coli.

Authors:  M Durairaj; S E Jensen
Journal:  J Ind Microbiol       Date:  1996-03

4.  Photoaffinity labelling of isopenicillin N synthetase.

Authors:  J E Baldwin; J B Coates; M G Moloney; A J Pratt; A C Willis
Journal:  Biochem J       Date:  1990-03-01       Impact factor: 3.857

5.  Cloning and expression in Escherichia coli of isopenicillin N synthetase genes from Streptomyces lipmanii and Aspergillus nidulans.

Authors:  B J Weigel; S G Burgett; V J Chen; P L Skatrud; C A Frolik; S W Queener; T D Ingolia
Journal:  J Bacteriol       Date:  1988-09       Impact factor: 3.490

6.  Photoaffinity labelling of isopenicillin N synthetase by laser-flash photolysis.

Authors:  J E Baldwin; J B Coates; J B Halpern; M G Moloney; A J Pratt
Journal:  Biochem J       Date:  1989-07-01       Impact factor: 3.857

7.  Production of Streptomyces clavuligerus isopenicillin N synthase in Escherichia coli using two-cistron expression systems.

Authors:  J L Doran; B K Leskiw; A K Petrich; D W Westlake; S E Jensen
Journal:  J Ind Microbiol       Date:  1990-06

8.  Beta-lactam biosynthesis in a gram-negative eubacterium: purification and characterization of isopenicillin N synthase from Flavobacterium sp. strain SC 12.154.

Authors:  H Palissa; H von Döhren; H Kleinkauf; H H Ting; J E Baldwin
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

Review 9.  Regulation and compartmentalization of β-lactam biosynthesis.

Authors:  Juan F Martín; Ricardo V Ullán; Carlos García-Estrada
Journal:  Microb Biotechnol       Date:  2009-05-31       Impact factor: 5.813
  9 in total

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