Immunocytochemical evidence for alpha-melanocyte-stimulating hormone in the hypothalamus of the frog Rana esculenta.

The distribution of immunoreactive alpha-melanocyte-stimulating hormone (alpha-MSH) within the brain of the frog, Rana esculenta, has been studied on adjacent serial sections using an indirect immunofluorescence technique. Immunoreactive cell bodies are found in the anterior part of the preoptic nucleus and in some ventral subependymal cerebrospinal fluid-contacting elements, and in the nucleus infundibularis ventralis. Numerous alpha-MSH-like immunoreactive fibers are present in the preoptic area, in the pars ventralis of the tuber cinereum, and in the outer layer of the median eminence. This staining pattern is completely eliminated after preabsorbing the antiserum with the corresponding antigen, but blocking tests with alpha-MSH-related peptides do not lead to any change in the immunoreaction. From these results it may be inferred that an alpha-MSH-like system is present in the hypothalamic neurosecretory area of R. esculenta, and is probably related to its hypophysiotropic functions. The results are compared to the distribution of alpha-MSH within the hypothalamus of reptiles and mammals.