Jutta Pretscher1, Matthias Ruebner1, Arif B Ekici2, Melanie Rödl1, Hanna Huebner1, Judith Schwitulla1, Adriana Titzmann1, Charlotte Hartwig1, Matthias W Beckmann1, Peter A Fasching1, Michael O Schneider1, Eva Schwenke3. 1. Department of Gynecology and Obstetrics, Erlangen University Hospital, Comprehensive Cancer Center Erlangen-EMN, Friedrich Alexander University of Erlangen-Nürnberg (FAU), Erlangen, Germany. 2. Institute of Human Genetics, Erlangen University Hospital, Friedrich Alexander University of Erlangen-Nürnberg (FAU), Erlangen, Germany. 3. Department of Gynecology and Obstetrics, Erlangen University Hospital, Comprehensive Cancer Center Erlangen-EMN, Friedrich Alexander University of Erlangen-Nürnberg (FAU), Erlangen, Germany. eva.schwenke@uk-erlangen.de.
Abstract
PURPOSE: Hypertensive pregnancy disorders and preeclampsia are major causes of maternal and fetal morbidity and mortality worldwide. Many different organs are involved in the diseases' clinical phenotype. The underlying mechanism is still unknown, with a possible genetic component. This case-control study investigated effects on the risk of preeclampsia of genetic variations (single nucleotide polymorphisms, SNPs) in the estrogen and progesterone pathway genes. METHODS: The study included 167 patients with preeclampsia and 115 healthy controls from the "Franconian Maternal Health Evaluation Studies" (FRAMES). All patients completed an epidemiological questionnaire, data from which were correlated with prospective data on pregnancy and labor. DNA was isolated from blood samples and genotyping was done by PCR. Variants in the aromatase gene CYP19A1 (rs10046, rs4646), progesterone receptor gene (rs1042838, rs10895068), and estrogen receptor-α gene (rs488133) were examined, and the genotype distribution in the two groups was analyzed statistically. RESULTS: A significant difference in the distribution frequency of genotypes between preeclampsia patients and controls was identified in one of the five SNPs. For rs10895068 in the progesterone receptor gene, genotype G/A was significantly more frequent among cases than controls (P = 0.023). No significant differences between the two cohorts were found in the other SNPs. CONCLUSIONS: This study showed a significant association between only one SNP in the progesterone receptor and preeclampsia. Other studies have also noted genetic aspects of preeclampsia. The underlying mechanism and causal relationship are not yet known, and further research is needed to explain the extent of genetic variations and the causal relationship in preeclampsia.
PURPOSE:Hypertensive pregnancy disorders and preeclampsia are major causes of maternal and fetal morbidity and mortality worldwide. Many different organs are involved in the diseases' clinical phenotype. The underlying mechanism is still unknown, with a possible genetic component. This case-control study investigated effects on the risk of preeclampsia of genetic variations (single nucleotide polymorphisms, SNPs) in the estrogen and progesterone pathway genes. METHODS: The study included 167 patients with preeclampsia and 115 healthy controls from the "Franconian Maternal Health Evaluation Studies" (FRAMES). All patients completed an epidemiological questionnaire, data from which were correlated with prospective data on pregnancy and labor. DNA was isolated from blood samples and genotyping was done by PCR. Variants in the aromatase gene CYP19A1 (rs10046, rs4646), progesterone receptor gene (rs1042838, rs10895068), and estrogen receptor-α gene (rs488133) were examined, and the genotype distribution in the two groups was analyzed statistically. RESULTS: A significant difference in the distribution frequency of genotypes between preeclampsia patients and controls was identified in one of the five SNPs. For rs10895068 in the progesterone receptor gene, genotype G/A was significantly more frequent among cases than controls (P = 0.023). No significant differences between the two cohorts were found in the other SNPs. CONCLUSIONS: This study showed a significant association between only one SNP in the progesterone receptor and preeclampsia. Other studies have also noted genetic aspects of preeclampsia. The underlying mechanism and causal relationship are not yet known, and further research is needed to explain the extent of genetic variations and the causal relationship in preeclampsia.
Entities:
Keywords:
Genetic marker; Preeclampsia; Pregnancy; Single nucleotide polymorphism (SNP)
Authors: Alison M Dunning; Catherine S Healey; Caroline Baynes; Ana-Teresa Maia; Serena Scollen; Ana Vega; Raquel Rodríguez; Nuno L Barbosa-Morais; Bruce A J Ponder; Yen-Ling Low; Sheila Bingham; Christopher A Haiman; Loic Le Marchand; Annegien Broeks; Marjanka K Schmidt; John Hopper; Melissa Southey; Matthias W Beckmann; Peter A Fasching; Julian Peto; Nichola Johnson; Stig E Bojesen; Børge Nordestgaard; Roger L Milne; Javier Benitez; Ute Hamann; Yon Ko; Rita K Schmutzler; Barbara Burwinkel; Peter Schürmann; Thilo Dörk; Tuomas Heikkinen; Heli Nevanlinna; Annika Lindblom; Sara Margolin; Arto Mannermaa; Veli-Matti Kosma; Xiaoqing Chen; Amanda Spurdle; Jenny Change-Claude; Dieter Flesch-Janys; Fergus J Couch; Janet E Olson; Gianluca Severi; Laura Baglietto; Anne-Lise Børresen-Dale; Vessela Kristensen; David J Hunter; Susan E Hankinson; Peter Devilee; Maaike Vreeswijk; Jolanta Lissowska; Louise Brinton; Jianjun Liu; Per Hall; Daehee Kang; Keun-Young Yoo; Chen-Yang Shen; Jyh-Cherng Yu; Hoda Anton-Culver; Argyrios Ziogoas; Alice Sigurdson; Jeff Struewing; Douglas F Easton; Montserrat Garcia-Closas; Manjeet K Humphreys; Jonathan Morrison; Paul D P Pharoah; Karen A Pooley; Georgia Chenevix-Trench Journal: Hum Mol Genet Date: 2009-01-06 Impact factor: 6.150