Jiang Du1, Yefang Diao2, Allah Rakha3, Fuad Ameen4, Muneera D F AlKahtani5, Atif Adnan6. 1. Department of Pathology, School of Basic Medical Sciences, China Medical University, Shenyang, China. 2. Teaching Affairs Office, International Education School, China Medical University, Shenyang, P.R. China. 3. Department of Forensic Sciences, University of Health Sciences Lahore, Lahore, Pakistan. 4. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia. 5. Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia. 6. Department of Human Anatomy, College of Basic Medical Science, China Medical University, Shenyang, P.R. China.
Abstract
BACKGROUND: Microsatellites or short tandem repeats (STRs) are considered the gold standard for forensic investigations and autosomal STRs are used for routine forensic personal identification. AIM: To provide a precise population database on an extended set of STRs which has never been done before and explore the forensic characteristics of 20 autosomal STRs. SUBJECTS AND METHODS: In the current study, we explored the genetic characteristics of 20 STRs loci in 1138 unrelated Han individuals using Goldeneye® 20A multiplex amplification system kit in the Liaoning Han population. Additionally, phylogenetic analysis based on the Nei's standard genetic distance was performed between the Han population and other relevant populations. RESULTS: A total of 253 alleles were observed while allelic frequencies ranged from 0.00043 to 0.5369. The combined discrimination power was 99.99999999999999999999789% and the combined exclusion power was 99.999998231%. Most of the loci were in HWE while only five pairs were out of LE. Population genetic analysis showed that the Han population has similarities with other East Asian populations. CONCLUSION: GoldenEyeTM 20A kit detects high diversity in the Liaoning Han population. These STRs which are part of this kit can be used for forensic investigations. Population genetic analysis showed that the Han population is different from the minority populations of Xinjiang.
BACKGROUND:Microsatellites or short tandem repeats (STRs) are considered the gold standard for forensic investigations and autosomal STRs are used for routine forensic personal identification. AIM: To provide a precise population database on an extended set of STRs which has never been done before and explore the forensic characteristics of 20 autosomal STRs. SUBJECTS AND METHODS: In the current study, we explored the genetic characteristics of 20 STRs loci in 1138 unrelated Han individuals using Goldeneye® 20A multiplex amplification system kit in the Liaoning Han population. Additionally, phylogenetic analysis based on the Nei's standard genetic distance was performed between the Han population and other relevant populations. RESULTS: A total of 253 alleles were observed while allelic frequencies ranged from 0.00043 to 0.5369. The combined discrimination power was 99.99999999999999999999789% and the combined exclusion power was 99.999998231%. Most of the loci were in HWE while only five pairs were out of LE. Population genetic analysis showed that the Han population has similarities with other East Asian populations. CONCLUSION: GoldenEyeTM 20A kit detects high diversity in the Liaoning Han population. These STRs which are part of this kit can be used for forensic investigations. Population genetic analysis showed that the Han population is different from the minority populations of Xinjiang.
Authors: Yan-Mei Huang; Jie Wang; Zhangping Jiao; Liu Yang; Xinning Zhang; Hui Tang; Yacheng Liu Journal: Int J Legal Med Date: 2013-03-13 Impact factor: 2.686