Samah Abdelrahman Hassan1,2, Mohamed Abdelrahman Arbab2,3, Sheren Faisal Abdelrahman4, SawsanAhmed Hamed Aldeaf2, Alsadig Fadallah Gassoum2, Hassan Hussein Musa5,6. 1. Almugtaribeen University Department of Medical Microbiology, Faculty of Medicine Khartoum Sudan. 2. National Center for Neurological Sciences Khartoum Sudan. 3. Faculty of Medicine, University of Khartoum Department of Surgery Khartoum Sudan. 4. Al Neelain University Faculty of Medical Laboratory Sciences Khartoum Sudan. 5. University of Khartoum Department of Medical Microbiology, Faculty of Medical Laboratory Sciences Khartoum Sudan. 6. Darfur College Biomedical Research Institute Nyala Sudan.
Abstract
BACKGROUND: Traumatic brain injuries (TBIs) is a leading cause of death, disability, and resources consumption. Cerebral hemorrhagic contusions are primary brain lesion and often one of the most visible lesions following TBIs. Interleukin-one beta (IL-1 β) is pro-inflammatory cytokines it is circulatory level and gene have been implicated in secondary brain injury and worse outcome following TBIs. This study is to determine the significance role of IL-1 β gene polymorphism (-511C/T) and circulatory level for prediction trauma severity and outcome in traumatic cerebral hemorrhagic contusion. METHODS: The study population includes 90 Sudanese patients with traumatic cerebral hemorrhagic and 90 apparently healthy individuals as control. IL-1β serum concentration was measured using enzyme-linked immunosorbent assay and IL-1β gene was genotyped using restriction fragment length polymorphism-polymerase chain reaction. RESULTS: Significant elevation of IL-1β level was seen among trauma patients compared to control (p-value < 0.001). Although there was no significant association between IL-1β level with trauma severity or death; IL-1β level was higher in severe brain injures compared with moderate and mild one, and the mean concentration of IL-1β was high (18.75 pg/mL) among patient developed poor outcome compared to survivals (15.17 pg/mL). T recessive allele of IL-1 β gene was detected in 13.3% of participant. The highest circulatory level of IL-1β (17.8 pg/mL) was observed among patients with TT homozygous alleles. IL-1 β gene polymorphism was not associated with trauma severity and death. CONCLUSIONS: IL-1β circulatory level was varied according to trauma severity and highly levels were seen among patients developed unfavorable outcome. IL-1β -511C/T gene was not associated with trauma severity and outcome.
BACKGROUND: Traumatic brain injuries (TBIs) is a leading cause of death, disability, and resources consumption. Cerebral hemorrhagic contusions are primary brain lesion and often one of the most visible lesions following TBIs. Interleukin-one beta (IL-1 β) is pro-inflammatory cytokines it is circulatory level and gene have been implicated in secondary brain injury and worse outcome following TBIs. This study is to determine the significance role of IL-1 β gene polymorphism (-511C/T) and circulatory level for prediction trauma severity and outcome in traumatic cerebral hemorrhagic contusion. METHODS: The study population includes 90 Sudanese patients with traumatic cerebral hemorrhagic and 90 apparently healthy individuals as control. IL-1β serum concentration was measured using enzyme-linked immunosorbent assay and IL-1β gene was genotyped using restriction fragment length polymorphism-polymerase chain reaction. RESULTS: Significant elevation of IL-1β level was seen among trauma patients compared to control (p-value < 0.001). Although there was no significant association between IL-1β level with trauma severity or death; IL-1β level was higher in severe brain injures compared with moderate and mild one, and the mean concentration of IL-1β was high (18.75 pg/mL) among patient developed poor outcome compared to survivals (15.17 pg/mL). T recessive allele of IL-1 β gene was detected in 13.3% of participant. The highest circulatory level of IL-1β (17.8 pg/mL) was observed among patients with TT homozygous alleles. IL-1 β gene polymorphism was not associated with trauma severity and death. CONCLUSIONS: IL-1β circulatory level was varied according to trauma severity and highly levels were seen among patients developed unfavorable outcome. IL-1β -511C/T gene was not associated with trauma severity and outcome.
Authors: E M El-Omar; M Carrington; W H Chow; K E McColl; J H Bream; H A Young; J Herrera; J Lissowska; C C Yuan; N Rothman; G Lanyon; M Martin; J F Fraumeni; C S Rabkin Journal: Nature Date: 2001-07-05 Impact factor: 49.962
Authors: Hongmin Chen; Leon M Wilkins; Nazneen Aziz; Christopher Cannings; David H Wyllie; Colin Bingle; John Rogus; James D Beck; Steven Offenbacher; Michael J Cork; Maryam Rafie-Kolpin; Chung-Ming Hsieh; Kenneth S Kornman; Gordon W Duff Journal: Hum Mol Genet Date: 2006-01-06 Impact factor: 6.150
Authors: Luiz Carlos Brasiliano Ferreira; Andrea Regner; Karen Dal Lago Miotto; Silvana de Moura; Nilo Ikuta; Andréia Escosteguy Vargas; José Artur Bogo Chies; Daniel Simon Journal: Brain Inj Date: 2014-05-15 Impact factor: 2.311
Authors: M Uzan; T Tanriverdi; O Baykara; A Kafadar; G Z Sanus; E Tureci; C Ozkara; O Uysal; N Buyra Journal: Acta Neurochir (Wien) Date: 2005-05-20 Impact factor: 2.216
Authors: Isabelle Bardou; Holly M Brothers; Roxanne M Kaercher; Sarah C Hopp; Gary L Wenk Journal: Neurobiol Aging Date: 2013-04-30 Impact factor: 4.673
Authors: Taís Frederes Krämer Alcalde; Andrea Regner; Edison Moraes Rodrigues Filho; Patrícia Corso Silveira; Gabriela Gonçalves Grossi; Daniel Simon Journal: Rev Bras Ter Intensiva Date: 2009-12