Mutita Junking1,2, Thidarath Rattanaburee1,3, Aussara Panya4,5, Irina Budunova6, Guy Haegeman7, Pa-Thai Yenchitsomanus1,2. 1. Division of Molecular Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. 2. Siriraj Center of Research Excellence for Cancer Immunotherapy (SiCORE-CIT), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. 3. Graduate Program in Immunology, Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. 4. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand. 5. Center of Excellence in Bioresources for Agriculture, Industry and Medicine, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand. 6. Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA. 7. Laboratory of Eukaryotic Gene Expression and Signal Transduction (LEGEST), Department of Physiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
Abstract
BACKGROUND: Cholangiocarcinoma (CCA) is a fatal cancer with high resistance to anticancer drugs. The development of new drugs or compounds to be used alone or in combination with currently available chemotherapeutic agents to improve the treatment of CCA is needed. Compound A (CpdA), which is a small plant-derived glucocorticoid receptor modulator, strongly inhibited the growth and survival of several cancers. However, the effect of CpdA on cholangiocarcinoma has not been elucidated. The aim of this study was to investigate the effect of CpdA on CCA. METHODS: Cytotoxicity of CpdA was tested in primary cells including peripheral blood mononuclear cells (PBMCs), fibroblasts, and human umbilical vein endothelial cells (HUVECs), as well as on CCA cell lines (KKU-100, KKU-055, and KKU-213) was examined. Cell cycle distribution and IL-6 expression was assessed by flow cytometry and real-time polymerase chain reaction, respectively. The effect of combination CpdA and cisplatin was evaluated by cell viability assay. RESULTS: CpdA significantly inhibited cell cycle at G1 phase in CCA cell lines, and reduced IL-6 mRNA expression. However, combination CpdA and cisplatin did not enhance the inhibitory effect. TGFβR-II expression was increased in CCA cells after the combination treatment. CONCLUSIONS: These results indicate the potential of CpdA for CCA treatment. However, combination treatment with CpdA and cisplatin increased CCA cell survival. The molecular mechanism is likely attributable to promotes cell survival via the TGFβR-II signaling pathway. The combination of CpdA with other anticancer drugs for CCA treatment should be further examined.
BACKGROUND:Cholangiocarcinoma (CCA) is a fatal cancer with high resistance to anticancer drugs. The development of new drugs or compounds to be used alone or in combination with currently available chemotherapeutic agents to improve the treatment of CCA is needed. Compound A (CpdA), which is a small plant-derived glucocorticoid receptor modulator, strongly inhibited the growth and survival of several cancers. However, the effect of CpdA on cholangiocarcinoma has not been elucidated. The aim of this study was to investigate the effect of CpdA on CCA. METHODS:Cytotoxicity of CpdA was tested in primary cells including peripheral blood mononuclear cells (PBMCs), fibroblasts, and human umbilical vein endothelial cells (HUVECs), as well as on CCA cell lines (KKU-100, KKU-055, and KKU-213) was examined. Cell cycle distribution and IL-6 expression was assessed by flow cytometry and real-time polymerase chain reaction, respectively. The effect of combination CpdA and cisplatin was evaluated by cell viability assay. RESULTS:CpdA significantly inhibited cell cycle at G1 phase in CCA cell lines, and reduced IL-6 mRNA expression. However, combination CpdA and cisplatin did not enhance the inhibitory effect. TGFβR-II expression was increased in CCA cells after the combination treatment. CONCLUSIONS: These results indicate the potential of CpdA for CCA treatment. However, combination treatment with CpdA and cisplatin increased CCA cell survival. The molecular mechanism is likely attributable to promotes cell survival via the TGFβR-II signaling pathway. The combination of CpdA with other anticancer drugs for CCA treatment should be further examined.
Entities:
Keywords:
Bile duct cancer; Compound A; Glucocorticoid; Interleukin-6; cholangiocarcinoma
Authors: Laurent L Reber; François Daubeuf; Maud Plantinga; Lode De Cauwer; Sarah Gerlo; Wim Waelput; Serge Van Calenbergh; Jan Tavernier; Guy Haegeman; Bart N Lambrecht; Nelly Frossard; Karolien De Bosscher Journal: J Immunol Date: 2012-03-05 Impact factor: 5.422
Authors: Alexander Rauch; Valerie Gossye; Debby Bracke; Elien Gevaert; Peggy Jacques; Katrien Van Beneden; Bernard Vandooren; Martina Rauner; Lorenz C Hofbauer; Guy Haegeman; Dirk Elewaut; Jan P Tuckermann; Karolien De Bosscher Journal: FASEB J Date: 2011-01-13 Impact factor: 5.191
Authors: Karolien De Bosscher; Wim Vanden Berghe; Ilse M E Beck; Wim Van Molle; Nathalie Hennuyer; Janet Hapgood; Claude Libert; Bart Staels; Ann Louw; Guy Haegeman Journal: Proc Natl Acad Sci U S A Date: 2005-10-21 Impact factor: 11.205