Literature DB >> 329836

Chitin biosynthesis in protoplasts and subcellular fractions of Aspergillus fumigatus.

D B Archer.   

Abstract

The biosynthesis of chitin has been obtained in broken mycelia and protoplasts of the fungus Aspergillus fumigatus. The specific activity of chitin synthase (EC 2.4.1.16) in a membrane preparation from protoplasts derived from the hyphal tips of A. fumigatus was 26.8-fold greater than that of the chitin synthase in broken mycelia, indicating that the active chitin synthase is located primarily in a membrane-bound site at the hyphal tip. Polyoxin D was a potent competitive inhibitor of the enzyme, having Ki 5.2 +/- 0.8 micron with respect to the natural substrate UDP-N-acetyl-D-glucosamine, which has Km 1.58 mM.

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Year:  1977        PMID： 329836      PMCID： PMC1164843          DOI： 10.1042/bj1640653

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  17 in total

1.  Synthesis of chitin by particulate preparations from Aspergillus flavus.

Authors:  E López-Romero; J Ruiz-Herrara
Journal:  Antonie Van Leeuwenhoek       Date:  1976       Impact factor: 2.271

2.  The synthesis of chitin in cell-free extracts of Neurospora crassa.

Authors:  L GLASER; D H BROWN
Journal:  J Biol Chem       Date:  1957-10       Impact factor: 5.157

3.  Chitin synthase in Mortierella vinacea: properties, cellular location and synthesis in growing cultures.

Authors:  J F Peberdy; P M Moore
Journal:  J Gen Microbiol       Date:  1975-10

4.  Effect of polyoxin D on chitin synthesis and septum formation in Saccharomyces cerevisiae.

Authors:  B Bowers; G Levin; E Cabib
Journal:  J Bacteriol       Date:  1974-08       Impact factor: 3.490

5.  Chitin synthetase activating factor from yeast, a protease.

Authors:  E Cabib; R Ulane
Journal:  Biochem Biophys Res Commun       Date:  1973-01-04       Impact factor: 3.575

6.  Glycoprotein enzymes secreted by Aspergillus fumigatus. Purification and properties of beta-glucosidase.

Authors:  M J Rudick; A D Elbein
Journal:  J Biol Chem       Date:  1973-09-25       Impact factor: 5.157

7.  Studies on polyoxins, antifungal antibiotics. 13. The structure of polyoxins.

Authors:  K Isono; K Asahi; S Suzuki
Journal:  J Am Chem Soc       Date:  1969-12-17       Impact factor: 15.419

8.  Glycoprotein enzymes secreted by Aspergillus fumigatus: purification and properties of a second beta-glucosidase.

Authors:  M J Rudick; A D Elbein
Journal:  J Bacteriol       Date:  1975-10       Impact factor: 3.490

9.  The synthesis of chitin by particulate preparations of Allomyces macrogynus.

Authors:  C A Porter; E G Jaworski
Journal:  Biochemistry       Date:  1966-04       Impact factor: 3.162

10.  Mechanism of action of the antifugal agent polyoxin D.

Authors:  A Endo; K Kakiki; T Misato
Journal:  J Bacteriol       Date:  1970-10       Impact factor: 3.490
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  9 in total

Review 1.  Recent advances in the understanding of the Aspergillus fumigatus cell wall.

Authors:  Mark J Lee; Donald C Sheppard
Journal:  J Microbiol       Date:  2016-02-27       Impact factor: 3.422

Review 2.  Biosynthesis of cell walls of fungi.

Authors:  V Farkas
Journal:  Microbiol Rev       Date:  1979-06

3.  Reshuffling of Aspergillus fumigatus cell wall components chitin and β-glucan under the influence of caspofungin or nikkomycin Z alone or in combination.

Authors:  Patricia E B Verwer; M L van Duijn; M Tavakol; Irma A J M Bakker-Woudenberg; Wendy W J van de Sande
Journal:  Antimicrob Agents Chemother       Date:  2011-12-27       Impact factor: 5.191

4.  Enhancement of the diversity of polyoxins by a thymine-7-hydroxylase homolog outside the polyoxin biosynthesis gene cluster.

Authors:  Changming Zhao; Tingting Huang; Wenqing Chen; Zixin Deng
Journal:  Appl Environ Microbiol       Date:  2010-09-03       Impact factor: 4.792

Review 5.  Compounds active against cell walls of medically important fungi.

Authors:  R F Hector
Journal:  Clin Microbiol Rev       Date:  1993-01       Impact factor: 26.132

6.  A multigene family related to chitin synthase genes of yeast in the opportunistic pathogen Aspergillus fumigatus.

Authors:  E Mellado; A Aufauvre-Brown; C A Specht; P W Robbins; D W Holden
Journal:  Mol Gen Genet       Date:  1995-02-06

7.  The antifungal echinocandin caspofungin acetate kills growing cells of Aspergillus fumigatus in vitro.

Authors:  J C Bowman; P Scott Hicks; M B Kurtz; H Rosen; D M Schmatz; P A Liberator; C M Douglas
Journal:  Antimicrob Agents Chemother       Date:  2002-09       Impact factor: 5.191

8.  Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis.

Authors:  Isabella Weber; Daniela Assmann; Eckhard Thines; Gero Steinberg
Journal:  Plant Cell       Date:  2005-11-28       Impact factor: 11.277

Review 9.  Methods for genetic transformation of filamentous fungi.

Authors:  Dandan Li; Yu Tang; Jun Lin; Weiwen Cai
Journal:  Microb Cell Fact       Date:  2017-10-03       Impact factor: 5.328
  9 in total

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