BACKGROUND: SARS-CoV-2 infection is associated with myocardial injury, but there is a paucity of experimental platforms for the condition.Methods and Results: Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) infected by SARS-CoV-2 for 3 days ceased beating and exhibited cytopathogenic changes with reduced viability. Active viral replication was evidenced by an increase in supernatant SARS-CoV-2 and the presence of SARS-CoV-2 nucleocaspid protein within hiPSC-CMs. Expressions of BNP, CXCL1, CXCL2, IL-6, IL-8 and TNF-α were upregulated, while ACE2 was downregulated. CONCLUSIONS: Our hiPSC-CM-based in-vitro SARS-CoV-2 myocarditis model recapitulated the cytopathogenic effects and cytokine/chemokine response. It could be exploited as a drug screening platform.
BACKGROUND:SARS-CoV-2 infection is associated with myocardial injury, but there is a paucity of experimental platforms for the condition.Methods and Results:Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) infected by SARS-CoV-2 for 3 days ceased beating and exhibited cytopathogenic changes with reduced viability. Active viral replication was evidenced by an increase in supernatant SARS-CoV-2 and the presence of SARS-CoV-2 nucleocaspid protein within hiPSC-CMs. Expressions of BNP, CXCL1, CXCL2, IL-6, IL-8 and TNF-α were upregulated, while ACE2 was downregulated. CONCLUSIONS: Our hiPSC-CM-based in-vitro SARS-CoV-2 myocarditis model recapitulated the cytopathogenic effects and cytokine/chemokine response. It could be exploited as a drug screening platform.
Authors: María Verónica Cuevas-Gonzalez; Álvaro Garcia-Perez; Álvaro Edgar Gonzalez-Aragon Pineda; León Francisco Espinosa-Cristobal; Alejandro Donohue-Cornejo; Karla Lizette Tovar-Carrillo; Rosa Alicia Saucedo-Acuña; Juan Carlos Cuevas-Gonzalez Journal: Biomed Res Int Date: 2021-08-25 Impact factor: 3.411
Authors: José Alexandre Salerno; Thayana Torquato; Jairo R Temerozo; Livia Goto-Silva; Karina Karmirian; Mayara A Mendes; Carolina Q Sacramento; Natalia Fintelman-Rodrigues; Letícia R Q Souza; Isis M Ornelas; Carla P Veríssimo; Luiz Guilherme H S Aragão; Gabriela Vitória; Carolina S G Pedrosa; Suelen da Silva Gomes Dias; Vinicius Cardoso Soares; Teresa Puig-Pijuan; Vinícius Salazar; Rafael Dariolli; Diogo Biagi; Daniel R Furtado; Luciana Barreto Chiarini; Helena L Borges; Patrícia T Bozza; Marilia Zaluar P Guimarães; Thiago M L Souza; Stevens K Rehen Journal: PeerJ Date: 2021-12-20 Impact factor: 2.984
Authors: Bhurint Siripanthong; Babken Asatryan; Thomas C Hanff; Salman R Chatha; Mohammed Y Khanji; Fabrizio Ricci; Daniele Muser; Victor A Ferrari; Saman Nazarian; Pasquale Santangeli; Rajat Deo; Leslie T Cooper; Saidi A Mohiddin; C Anwar A Chahal Journal: JACC Basic Transl Sci Date: 2022-02-09