Literature DB >> 32980480

Detection of mitochondria-pertinent components in exosomes.

Xiaowan Wang1, Ian Weidling1, Scott Koppel1, Blaise Menta1, Judit Perez Ortiz1, Anuradha Kalani1, Heather M Wilkins1, Russell H Swerdlow2.   

Abstract

We screened cell line and plasma-derived exosomes for molecules that localize to mitochondria or that reflect mitochondrial integrity. SH-SY5Y cell-derived exosomes contained humanin, citrate synthase, and fibroblast growth factor 21 protein, and plasma-derived exosomes contained humanin, voltage-dependent anion-selective channel 1, and transcription factor A protein. Nuclear mitochondrial (NUMT) DNA complicated analyses of mitochondrial DNA (mtDNA), which otherwise suggested exosomes contain at most very low amounts of extended mtDNA sequences but likely contain degraded pieces of mtDNA. Cell and plasma-derived exosomes contained several mtDNA-derived mRNA sequences, including those for ND2, CO2, and humanin. These results can guide exosome-focused, mitochondria-pertinent biomarker development.
Copyright © 2020 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

Entities:  

Keywords:  Biomarker; Exosome; Mitochondria; NUMT; mitochondrial DNA

Mesh：

Substances：

Year:  2020        PMID： 32980480      PMCID： PMC7669644          DOI： 10.1016/j.mito.2020.09.006

Source DB:  PubMed          Journal:  Mitochondrion        ISSN： 1567-7249            Impact factor:   4.160


  45 in total

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Authors:  Russell H Swerdlow; Gerard T Redpath; Daniel R Binder; John N Davis; Scott R VandenBerg
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Review 2.  Exosomes in bodily fluids are a highly stable resource of disease biomarkers.

Authors:  Stephanie Boukouris; Suresh Mathivanan
Journal:  Proteomics Clin Appl       Date:  2015-03-19       Impact factor: 3.494

3.  Lipidomic and proteomic analysis of exosomes from mouse cortical collecting duct cells.

Authors:  Viet D Dang; Kishore Kumar Jella; Ragy R T Ragheb; Nancy D Denslow; Abdel A Alli
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4.  Cetyltrimethyl ammonium bromide (CTAB) DNA miniprep for plant DNA isolation.

Authors:  Joseph D Clarke
Journal:  Cold Spring Harb Protoc       Date:  2009-03

5.  Quantification of peripheral whole blood, cell-free plasma and exosome encapsulated mitochondrial DNA copy numbers in patients with atrial fibrillation.

Authors:  Beáta Soltész; Réka Urbancsek; Ondrej Pös; Orsolya Hajas; Ildikó Noémi Forgács; Edina Szilágyi; Edina Nagy-Baló; Tomas Szemes; Zoltán Csanádi; Bálint Nagy
Journal:  J Biotechnol       Date:  2019-05-04       Impact factor: 3.307

Review 6.  Integrins and Exosomes, a Dangerous Liaison in Cancer Progression.

Authors:  Mayra Paolillo; Sergio Schinelli
Journal:  Cancers (Basel)       Date:  2017-07-26       Impact factor: 6.639

7.  Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next-generation sequencing.

Authors:  Dominik Buschmann; Benedikt Kirchner; Stefanie Hermann; Melanie Märte; Christine Wurmser; Florian Brandes; Stefan Kotschote; Michael Bonin; Ortrud K Steinlein; Michael W Pfaffl; Gustav Schelling; Marlene Reithmair
Journal:  J Extracell Vesicles       Date:  2018-06-04

8.  Comparative analysis of exosome isolation methods using culture supernatant for optimum yield, purity and downstream applications.

Authors:  Girijesh Kumar Patel; Mohammad Aslam Khan; Haseeb Zubair; Sanjeev Kumar Srivastava; Moh'd Khushman; Seema Singh; Ajay Pratap Singh
Journal:  Sci Rep       Date:  2019-03-29       Impact factor: 4.379

9.  Extracellular vesicles are increased in the serum of children with autism spectrum disorder, contain mitochondrial DNA, and stimulate human microglia to secrete IL-1β.

Authors:  Irene Tsilioni; Theoharis C Theoharides
Journal:  J Neuroinflammation       Date:  2018-08-27       Impact factor: 8.322

10.  Effect of pH, temperature and freezing-thawing on quantity changes and cellular uptake of exosomes.

Authors:  Yirui Cheng; Qingyu Zeng; Qing Han; Weiliang Xia
Journal:  Protein Cell       Date:  2019-04       Impact factor: 14.870
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  8 in total

1.  Pharmacologic enrichment of exosome yields and mitochondrial cargo.

Authors:  Xiaowan Wang; Alexandra Berkowicz; Kirsten King; Blaise Menta; Alexander P Gabrielli; Lesya Novikova; Benjamin Troutwine; Joseph Pleen; Heather M Wilkins; Russell H Swerdlow
Journal:  Mitochondrion       Date:  2022-04-06       Impact factor: 4.534

Review 2.  The History and Future of Basic and Translational Cell-Free DNA Research at a Glance.

Authors:  Peter B Gahan; Heidi Schwarzenbach; Philippe Anker
Journal:  Diagnostics (Basel)       Date:  2022-05-10

Review 3.  Exosomes: Innocent Bystanders or Critical Culprits in Neurodegenerative Diseases.

Authors:  Margarida Beatriz; Rita Vilaça; Carla Lopes
Journal:  Front Cell Dev Biol       Date:  2021-05-13

Review 4.  Exosomes in Immune Regulation.

Authors:  Heidi Schwarzenbach; Peter B Gahan
Journal:  Noncoding RNA       Date:  2021-01-08

Review 5.  The Molecular Structure and Role of Humanin in Neural and Skeletal Diseases, and in Tissue Regeneration.

Authors:  Sipin Zhu; Xiaoyong Hu; Samuel Bennett; Jiake Xu; Yuliang Mai
Journal:  Front Cell Dev Biol       Date:  2022-03-16

Review 6.  Mitochondrial Damage-Associated Molecular Patterns Content in Extracellular Vesicles Promotes Early Inflammation in Neurodegenerative Disorders.

Authors:  Cláudia M Deus; Henrique Tavares; Margarida Beatriz; Sandra Mota; Carla Lopes
Journal:  Cells       Date:  2022-08-01       Impact factor: 7.666

7.  Reduced Maternal Circulating Cell-Free Mitochondrial DNA Is Associated With the Development of Preeclampsia.

Authors:  Spencer C Cushen; Contessa A Ricci; Jessica L Bradshaw; Talisa Silzer; Alexandra Blessing; Jie Sun; Zhengyang Zhou; Sabrina M Scroggins; Mark K Santillan; Donna A Santillan; Nicole R Phillips; Styliani Goulopoulou
Journal:  J Am Heart Assoc       Date:  2022-01-11       Impact factor: 6.106

8.  A degradative to secretory autophagy switch mediates mitochondria clearance in the absence of the mATG8-conjugation machinery.

Authors:  Hayden Weng Siong Tan; Guang Lu; Han Dong; Yik-Lam Cho; Auginia Natalia; Liming Wang; Charlene Chan; Dennis Kappei; Reshma Taneja; Shuo-Chien Ling; Huilin Shao; Shih-Yin Tsai; Wen-Xing Ding; Han-Ming Shen
Journal:  Nat Commun       Date:  2022-06-28       Impact factor: 17.694
  8 in total

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