Alfredo Eduardo Maiorano1, Elda Sabino da Silva2, Rafael Firmani Perna3, Cristiane Angélica Ottoni4,5, Rosane Aparecida Moniz Piccoli2, Rubén Cuervo Fernandez6, Beatriz Guilarte Maresma6, Maria Filomena de Andrade Rodrigues2. 1. Bionanomanufacturing Center, Institute for Technological Research (IPT), Av. Prof. Almeida Prado 532, São Paulo, São Paulo, 05508-901, Brazil. maiorano@ipt.br. 2. Bionanomanufacturing Center, Institute for Technological Research (IPT), Av. Prof. Almeida Prado 532, São Paulo, São Paulo, 05508-901, Brazil. 3. Institute of Science and Technology, Federal University of Alfenas (ICT/UNIFAL-MG), José Aurélio Vilela Road, 11999 BR 267 Km 533, Poços de Caldas, Minas Gerais, 37715-400, Brazil. 4. Biosciences Institute, São Paulo State University (UNESP), Praça Infante Dom Henrique s/no, São Vicente, São Paulo, 11330-900, Brazil. 5. Instituto de Estudos Avançados do Mar (IEAMar), São Paulo State University, Av. João Frascisco Bensdorp 1178, São Vicente, São Paulo, 11350-011, Brazil. 6. Instituto Cubano de Investigaciones de los Derivados de la Caña de Azúcar (ICIDCA), Vía Blanca 804, Carretera Central, San Miguel del Padrón, 11000, Ciudad de la Habana, Cuba.
Abstract
OBJECTIVE: Fructooligosaccharides (FOS) are prebiotic substances that have been extensively incorporated in different products of food industry mostly for their bifidogenic properties and economic value. The main commercial FOS production comes from the biotransformation of sucrose and intracellular and extracellular microbial enzymes-fructosyltransferases (FTase). Aspergillus oryzae IPT-301 produces FTase. In order to increase its production, this study focuses on evaluating the effects of different agitation speed and aeration rates which affect yields in a stirred tank bioreactor. RESULTS: Agitation had more influence on cell growth than aeration. The maximum intracellular FTase activity and the volumetric productivity of total intracellular FTase were obtained at 800 rpm and 0.75 vvm, and reached values of 2100 U g-1 and 667 U dm-3 h-1, respectively. The agitation speed had a strong influence on the activity of extracellular FTase produced which reached the maximum amount of 53 U cm-3. The higher value of total activity obtained was 22,831 U dm-3 at 0.75 vvm and 800 rpm. CONCLUSION: Aeration rates and agitation speed showed strong influence upon the growth and production of fructosyltransferase from Aspergillus oryzae IPT-301 in media containing sucrose as carbon source. The control of aeration rate and agitation speed can be a valuable fermentation strategy to improve enzyme production.
OBJECTIVE:Fructooligosaccharides (FOS) are prebiotic substances that have been extensively incorporated in different products of food industry mostly for their bifidogenic properties and economic value. The main commercial FOS production comes from the biotransformation of sucrose and intracellular and extracellular microbial enzymes-fructosyltransferases (FTase). Aspergillus oryzaeIPT-301 produces FTase. In order to increase its production, this study focuses on evaluating the effects of different agitation speed and aeration rates which affect yields in a stirred tank bioreactor. RESULTS: Agitation had more influence on cell growth than aeration. The maximum intracellular FTase activity and the volumetric productivity of total intracellular FTase were obtained at 800 rpm and 0.75 vvm, and reached values of 2100 U g-1 and 667 U dm-3 h-1, respectively. The agitation speed had a strong influence on the activity of extracellular FTase produced which reached the maximum amount of 53 U cm-3. The higher value of total activity obtained was 22,831 U dm-3 at 0.75 vvm and 800 rpm. CONCLUSION: Aeration rates and agitation speed showed strong influence upon the growth and production of fructosyltransferase from Aspergillus oryzaeIPT-301 in media containing sucrose as carbon source. The control of aeration rate and agitation speed can be a valuable fermentation strategy to improve enzyme production.