| Literature DB >> 32975794 |
Preeti Manandhar1, Shivani Sachdev1, Marina Santiago2.
Abstract
Quantitative measurement of receptor signaling by different ligands is important for understanding the mechanism of drug action and screening of drugs. Here, we describe a simple and cost-effective method of measuring adenylyl cyclase inhibition, one of the hallmarks of opioid receptor activation. The assay is based on bioluminescence resonance energy transfer (BRET) that involves transfection of a biosensor in human embryonic kidney (HEK)-293 cells stably transfected with μ-opioid receptor (μ receptor), enabling real-time measurement of cAMP levels.Entities:
Keywords: Adenylyl cyclase; BRET assay; Forskolin; HEK-293 cells; cAMP; μ-Opioid receptor
Mesh：
Substances：
Year: 2021 PMID： 32975794 DOI： 10.1007/978-1-0716-0884-5_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745