Shubha Priyamvada1, Anoop Kumar1,2, Seema Saksena1,2, Hayat Onyuksel3. 1. Department of Medicine, College of Medicine, University of Illinois at Chicago, IL 60612, USA. 2. Jesse Brown VA Medical Center, Chicago, IL 60612, USA. 3. Department of Pharmaceutical Sciences, College of Pharmacy, University of Illinois at Chicago, IL 60612, USA.
Abstract
Aim: To evaluate the efficacy of locally delivered nanomedicine, vasoactive intestinal peptide in sterically stabilized micelles (VIP-SSM) to the colon and conduct in vitro release studies of a potential oral formulation. Materials & methods: Intracolonic instillation of VIP-SSM was tested in a mouse model of dextran sulfate sodium-induced colitis. Based on the effective mouse dose, human equivalent dose containing nanomedicine powder was filled into enteric coated capsules for in vitro release testing. Results: Colonic delivery of VIP-SSM significantly alleviated colitis. VIP-SSM containing capsules completely dissolved at colonic pH allowing micelles to reform with active VIP. Capsule formulations exhibited reproducible release profiles when stored up to 6 weeks demonstrating stability. Conclusion: VIP-SSM is an effective nanomedicine formulation which appears to have potential for oral treatment of colitis in humans. [Formula: see text].
Aim: To evaluate the efficacy of locally delivered nanomedicine, vasoactive intestinal peptide in sterically stabilized micelles (VIP-SSM) to the colon and conduct in vitro release studies of a potential oral formulation. Materials & methods: Intracolonic instillation of VIP-SSM was tested in a mouse model of dextran sulfate sodium-induced colitis. Based on the effective mouse dose, human equivalent dose containing nanomedicine powder was filled into enteric coated capsules for in vitro release testing. Results:Colonic delivery of VIP-SSM significantly alleviated colitis. VIP-SSM containing capsules completely dissolved at colonic pH allowing micelles to reform with active VIP. Capsule formulations exhibited reproducible release profiles when stored up to 6 weeks demonstrating stability. Conclusion:VIP-SSM is an effective nanomedicine formulation which appears to have potential for oral treatment of colitis in humans. [Formula: see text].
Authors: Xinfu Guan; Heidi E Karpen; John Stephens; John T Bukowski; Sanyong Niu; Guangcheng Zhang; Barbara Stoll; Milton J Finegold; Jens J Holst; Darryl Hadsell; Darry L Hadsell; Buford L Nichols; Douglas G Burrin Journal: Gastroenterology Date: 2006-01 Impact factor: 22.682
Authors: N J Chapman; M L Brown; S F Phillips; W J Tremaine; K W Schroeder; M K Dewanjee; A R Zinsmeister Journal: Mayo Clin Proc Date: 1992-03 Impact factor: 7.616