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Literature DB >> 3297049

Molecular cloning and over-expression of the glyoxylate bypass operon from Escherichia coli ML308.

E M el-Mansi, C MacKintosh, K Duncan, W H Holms, H G Nimmo.

Abstract

A recombinant plasmid carrying an 11 kb restriction-endonuclease-ClaI fragment of genomic DNA from Escherichia coli ML308 was constructed. This plasmid complements an aceA mutation. The plasmid encodes the structural genes of the glyoxylate bypass operon, namely malate synthase A (aceB), isocitrate lyase (aceA) and isocitrate dehydrogenase kinase/phosphatase (aceK), as judged by overexpression of enzyme activities and transcription/translation experiments in vitro. Subcloning confirmed that expression of the aceK gene is essential for growth on acetate.

Entities: Chemical Gene Species

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Year: 1987 PMID： 3297049 PMCID： PMC1147762 DOI： 10.1042/bj2420661

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

25 in total

1. Branch point control by the phosphorylation state of isocitrate dehydrogenase. A quantitative examination of fluxes during a regulatory transition.

Authors: K Walsh; D E Koshland
Journal: J Biol Chem Date: 1985-07-15 Impact factor: 5.157

2. Isolation of active and inactive forms of isocitrate dehydrogenase from Escherichia coli ML 308.

Authors: A C Borthwick; W H Holms; H G Nimmo
Journal: Eur J Biochem Date: 1984-06-01

3. The branch point effect. Ultrasensitivity and subsensitivity to metabolic control.

Authors: D C LaPorte; K Walsh; D E Koshland
Journal: J Biol Chem Date: 1984-11-25 Impact factor: 5.157

4. The phosphorylation of Escherichia coli isocitrate dehydrogenase in intact cells.

Authors: A C Borthwick; W H Holms; H G Nimmo
Journal: Biochem J Date: 1984-09-15 Impact factor: 3.857

5. A protein with kinase and phosphatase activities involved in regulation of tricarboxylic acid cycle.

Authors: D C LaPorte; D E Koshland
Journal: Nature Date: 1982-12-02 Impact factor: 49.962

6. Partial purification and properties of isocitrate dehydrogenase kinase/phosphatase from Escherichia coli ML308.

Authors: G A Nimmo; A C Borthwick; W H Holms; H G Nimmo
Journal: Eur J Biochem Date: 1984-06-01

7. The regulatory properties of isocitrate dehydrogenase kinase and isocitrate dehydrogenase phosphatase from Escherichia coli ML308 and the roles of these activities in the control of isocitrate dehydrogenase.

Authors: G A Nimmo; H G Nimmo
Journal: Eur J Biochem Date: 1984-06-01

8. Pyruvate metabolism and the phosphorylation state of isocitrate dehydrogenase in Escherichia coli.

Authors: E M el-Mansi; H G Nimmo; W H Holms
Journal: J Gen Microbiol Date: 1986-03

9. Elevated levels of glyoxylate shunt enzymes in Escherichia coli strains constitutive for fatty acid degradation.

Authors: S R Maloy; M Bohlander; W D Nunn
Journal: J Bacteriol Date: 1980-08 Impact factor: 3.490

10. Genetic regulation of the glyoxylate shunt in Escherichia coli K-12.

Authors: S R Maloy; W D Nunn
Journal: J Bacteriol Date: 1982-01 Impact factor: 3.490

11 in total

1. Glyoxylate bypass operon of Escherichia coli: cloning and determination of the functional map.

Authors: T Chung; D J Klumpp; D C LaPorte
Journal: J Bacteriol Date: 1988-01 Impact factor: 3.490

2. Nucleotide sequence and expression of the aceK gene coding for isocitrate dehydrogenase kinase/phosphatase in Escherichia coli.

Authors: J C Cortay; F Bleicher; C Rieul; H C Reeves; A J Cozzone
Journal: J Bacteriol Date: 1988-01 Impact factor: 3.490

3. Nucleotide sequence of the aceA gene coding for isocitrate lyase in Escherichia coli.

Authors: C Rieul; F Bleicher; B Duclos; J C Cortay; A J Cozzone
Journal: Nucleic Acids Res Date: 1988-06-24 Impact factor: 16.971

Review 4. Applications of recombinant DNA technology to studies of metabolic regulation.

Authors: H G Nimmo; P T Cohen
Journal: Biochem J Date: 1987-10-01 Impact factor: 3.857

5. Identification of a cysteine residue at the active site of Escherichia coli isocitrate lyase.

Authors: H G Nimmo; F Douglas; C Kleanthous; D G Campbell; C MacKintosh
Journal: Biochem J Date: 1989-07-15 Impact factor: 3.857

6. Inactivation of isocitrate lyase leads to increased production of medium-chain-length poly(3-hydroxyalkanoates) in Pseudomonas putida.

Authors: S Klinke; M Dauner; G Scott; B Kessler; B Witholt
Journal: Appl Environ Microbiol Date: 2000-03 Impact factor: 4.792

7. Preparation and properties of pure, full-length IclR protein of Escherichia coli. Use of time-of-flight mass spectrometry to investigate the problems encountered.

Authors: L J Donald; I V Chernushevich; J Zhou; A Verentchikov; N Poppe-Schriemer; D J Hosfield; J B Westmore; W Ens; H W Duckworth; K G Standing
Journal: Protein Sci Date: 1996-08 Impact factor: 6.725