Singularly Narrow Viral Size and Mobility Standards from the 38.3 nm Chronic Bee Paralysis Virus and Its 17.5 nm Satellite.

The chronic bee paralysis virus (CBPV), extracted from sick or dead bees, was studied by mobility measurements via electrospray charge reduction with a differential mobility analyzer (DMA) of unusually high resolution. Three different particles are observed. The most abundant one contributes a mobility peak at 38.3 nm, approximately as expected for CBPV. The peak is very sharp in spite of the nonisometric nature of CBPV. We also observe a previously unreported weaker well-resolved shoulder 4.8% more mobile, perhaps due to empty (genome-free) particles. Another sharp peak appearing at approximately 17.51 nm is likely associated with the known icosahedral CBPV satellite (CBPVS). The 17.51 and 38.3 nm peaks offer size and mobility standards much narrower than previously reported at any size above 5 nm, with relative full peak width at half-maximum (FWHM) in mobility approaching 2% (∼1% in diameter). Slight but clear imperfections in the DMA response and the electrospraying process suggest that the real width of the viral mobility distribution is less than 2%.