Clinical manifestations and epidemiology of adolescent tuberculosis in Ukraine.

BACKGROUND: During adolescence, childhood and adult forms of tuberculosis (TB) overlap, resulting in diverse disease manifestations. Knowing which patient characteristics are associated with which manifestations may facilitate diagnosis and enhance understanding of TB pathophysiology.
METHODS: In this cross-sectional study, we included 10-19-year-olds in Ukraine's national TB registry who started TB treatment between 2015 and 2018. Using multivariable regression, we estimated associations between patient characteristics and four presentations of TB: pleural, extrathoracic, cavitary and rifampicin-resistant (RR). We also described the epidemiology of adolescent TB in Ukraine.
RESULTS: Among 2491 adolescent TB cases, 88.4% were microbiologically confirmed. RR-TB was confirmed in 16.9% of new and 29.7% of recurrent cases. Of 88 HIV-infected adolescents, 59.1% were not on antiretroviral therapy at TB diagnosis. Among 10-14-year-olds, boys had more pleural disease (adjusted OR (aOR) 2.12, 95% CI: 1.08-4.37). Extrathoracic TB was associated with age 15-19 years (aOR 0.26, 95% CI: 0.18-0.37) and HIV (aOR 3.25, 95% CI: 1.55-6.61 in 10-14-year-olds; aOR 8.18, 95% CI: 3.58-17.31 in 15-19-year-olds). Cavitary TB was more common in migrants (aOR 3.53, 95% CI: 1.66-7.61) and 15-19-year-olds (aOR 4.10, 95% CI: 3.00-5.73); among 15-19-year-olds, it was inversely associated with HIV (aOR 0.32, 95% CI: 0.13-0.70). RR-TB was associated with recurrent disease (aOR 1.87, 95% CI: 1.08-3.13), urban residence (aOR 1.27, 95% CI: 1.01-1.62) and cavitation (aOR 2.98, 95% CI: 2.35-3.78).
CONCLUSIONS: Age, sex, HIV and social factors impact the presentation of adolescent TB. Preventive, diagnostic and treatment activities should take these factors into consideration.

Introduction

Adolescents—defined as people 10–19 years of age—account for an estimated 800 000 incident tuberculosis (TB) cases annually [1]. During adolescence, a transition from childhood forms of TB to adult-type TB occurs [2-7], resulting in diverse clinical presentations. Among adolescents with TB disease, 10–20% have pleural TB, 10–20% have extrathoracic TB, and approximately 25% have lung cavitation [6, 8–11]. The percentage of adolescents with rifampicin-resistant (RR) TB, which includes multidrug-resistant (MDR) TB (combined resistance to isoniazid and rifampicin), is largely unknown; however, data from several former Soviet countries have shown a higher RR-TB risk among adolescents and young adults than older age groups [12-14]. Pleural and extrathoracic TB are challenging to diagnose due to the difficulty of microbiological confirmation and nonspecific clinical presentations [15]. RR-TB is difficult to confirm in adolescents with culture-negative TB and in settings with limited drug susceptibility testing (DST). Both RR and cavitary TB require longer therapies and have worse outcomes. Knowing which patient characteristics are associated with pleural, extrathoracic and RR-TB may help clinicians make these challenging diagnoses. Identifying predictors of cavitary and RR-TB may help elucidate why some adolescents develop these severe disease forms, with the goal of identifying modifiable risk factors and therapeutic targets.

Our current understanding of adolescent TB comes primarily from observational studies conducted before the availability of anti-TB chemotherapy. These reports, along with a few recent publications, have described age- and sex-based patterns in clinical presentation, including more pleural TB in boys and a rise in lung cavitation in mid-adolescence [2-7]. Nonetheless, knowledge gaps remain. First, previous studies were descriptive and did not adjust for covariates. Second, since the pre-chemotherapy era, two major challenges to TB elimination have emerged: HIV and RR-TB [16]. We know little about how infection with HIV or RR Mycobacterium tuberculosis (Mtb) strains impacts adolescent TB presentation.

With its high incidence of RR-TB and TB/HIV coinfection, Ukraine is an ideal setting for evaluating the impact of these factors on adolescent TB. Analysing programmatic data from the Ukrainian National TB Program (NTP), we aimed to identify adolescent patient characteristics associated with pleural, extrathoracic, cavitary and RR-TB. As a secondary aim, we described the epidemiology of adolescent TB in Ukraine.

Methods

Setting

In 2018, Ukraine had an estimated TB incidence of 80 per 100 000 population; RR-TB prevalence was 29% among new and 46% among previously treated cases; and HIV-infected people accounted for 23% of all incident TB cases [16]. In a 2013–2014 national survey of anti-TB drug resistance, >40% of MDR-TB cases could be classified further as extensively drug-resistant (XDR) TB (additional resistance to a fluoroquinolone and a second-line injectable agent) or pre-XDR (additional resistance to a fluoroquinolone or a second-line injectable agent) [14].

According to Ukrainian NTP guidelines, evaluation for TB disease should include smear microscopy; mycobacterial culture; Xpert MTB/RIF (Cepheid, Sunnyvale, CA, USA); DST to rifampicin, isoniazid, ethambutol, pyrazinamide and streptomycin for culture-positive isolates; HIV testing; and posterior–anterior and lateral chest radiographs (CXRs) [17, 18]. Computed tomography scans are obtained only when CXR results are equivocal. In the absence of microbiological confirmation, TB may be diagnosed based on symptoms, imaging findings and contact with a known TB case. The NTP has no specific guidelines for diagnosing extrapulmonary TB.

Data source

Since 2013, the NTP has required clinicians to enter standardised data for every TB patient into eTB Manager, which is an online case registry. NTP staff downloaded de-identified data from eTB Manager on November 9, 2018. We collected the following variables recorded at treatment initiation: age; sex; HIV status; antiretroviral therapy (ART) start date, if applicable; whether the TB episode is new or recurrent; urban versus rural residence; oblast (Ukrainian equivalent of a province) of residence; migration status; history of incarceration; excess alcohol use; anatomic site(s) of TB disease; and results of imaging, smear microscopy, culture, Xpert and DST. HIV viral loads and CD4 counts are not recorded in eTB Manager and so were unavailable.

Definitions

Consistently with previous studies, we referred to adolescents as “younger” (10–14 years old) and “older” (15–19 years old) [1, 19, 20]. Participants were classified as living in “urban” (cities) or “rural” (suburbs, towns, villages, farms) areas. We grouped internally displaced persons, immigrants and refugees together as “migrants”. “Excess alcohol use” was not defined by any standard criteria and was self-reported. Moreover, data fields pertaining to migration and alcohol were completed only when positive; we assigned negative responses to empty fields. We classified oblasts into “conflict-affected” (Donetsk and Luhansk, sites of armed conflict with Russia since 2015) and “nonconflict-affected” (the rest of Ukraine) [21]. eTB Manager data for conflict-affected oblasts were available from the areas under Ukrainian control only.

We defined “recurrent TB” as disease in a participant who was previously treated for TB, whose most recent anti-TB regimen ended ≥6 months ago, and who was cured or completed treatment at the end of that regimen [22]. We used “intrathoracic” to refer to the lung parenchyma, intrathoracic lymph nodes, pleura and/or pericardium, and “extrathoracic” to refer to any other site [23]. “Cavitary TB” and “pleural TB” were diagnosed by imaging.

“Microbiologic confirmation” was characterised by documentation of positive smear, positive culture, positive Xpert and/or a DST result. We defined “baseline DST” as a DST performed on a sample obtained prior to or ≤30 days after treatment initiation.

Inclusion and exclusion criteria

We included people registered in eTB Manager who started a TB regimen between January 1, 2015 and November 9, 2018, and were 10–19 years old at treatment initiation. We excluded adolescents who began treatment before 2015 due to concerns about data quality during the first 2 years of eTB Manager's implementation. A participant could contribute >1 incident TB case if ≥6 months passed between the cure or treatment completion of one regimen and the initiation of a subsequent regimen.

Analysis

Using World Bank population estimates [24], we calculated the average annual age- and sex-specific reported TB incidence.

Using logistic regression, we estimated associations between patient characteristics at diagnosis and these clinical presentations: 1) TB with pleural involvement, compared to TB without pleural involvement; 2) TB with extrathoracic involvement, compared to exclusively intrathoracic TB; 3) cavitary pulmonary TB, compared to all other forms of TB; and 4) baseline RR-TB, compared to baseline rifampicin-susceptible (including isoniazid-monoresistant) TB. For the fourth outcome, we excluded subjects with no baseline DST result. We assessed the following predictors for all outcomes: age group, sex, HIV status, new versus recurrent TB, conflict-affected versus nonconflict-affected oblast, urban versus rural residence, migration status, history of incarceration and excess alcohol use. For the first three outcomes, we also included baseline rifampicin susceptibility. For the fourth outcome only, we added lung cavitation, which was associated with RR-TB in other former Soviet republics [25, 26].

Because age may modify the effect of sex and HIV infection on immune response to Mtb, we assessed interactions between age group and sex and HIV for all outcomes [27]. As different risk factors have been reported for transmitted versus acquired RR-TB [26, 28], we assessed interactions between DST result and new versus recurrent TB for the first three outcomes and between new versus recurrent TB and all other covariates for the fourth outcome. We built multivariable models by including all covariates and interaction terms with p<0.2 from univariable analysis.

HIV status and urban versus rural residence had small amounts of missing data, and half of the cases with missing HIV status had a documented HIV test date. Therefore, we assumed the lack of these variables was due to clerical errors and conducted complete-case analysis. For missing DST results, we used the missing indicator method to maximise the sample size in multivariable analysis for the first three outcomes. However, we did not report estimates for cases without a DST result because lack of data was mostly an artefact of the outcomes of interest (i.e. the four clinical presentations). Specifically, 289 of 328 (88.1%) cases without baseline DST result lacked documentation of smear or culture positivity; in these cases, we assumed DST could not be performed due to paucibacillary TB – which excludes cavitary disease – or inability to sample extrapulmonary sites. No other variables had missing values.

All analyses were conducted using R version 3.5.1.

Ethics

This study used nonidentifiable data collected during routine clinical care and, therefore, was deemed exempt from institutional review board (IRB) approval by Bogomolets National Medical University (Kyiv, Ukraine), Center for Public Health (Kyiv, Ukraine) and Boston University (Boston, MA, USA). The IRB of The Miriam Hospital (Providence, RI, USA) also approved this study and waived informed consent.

Results

During the study period, the average annual reported adolescent TB incidence was 7.4 per 100 000 for younger adolescents, 27.8 per 100 000 for older adolescents, and 17.7 per 100 000 for all adolescents. TB notification rate increased throughout adolescence for both sexes (figure 1). Notification rates were comparable for girls and boys between ages 10–13 years, higher in girls between ages 14–15 years and higher in boys thereafter.

FIGURE 1

Average annual tuberculosis notification rates in adolescents, stratified by age and sex, Ukraine, 2015–2017. Confidence intervals are omitted from this figure as we are presenting the actual notification rate rather than drawing inferences. Data from 2018 were excluded from this figure because there may be a delay of up to 3 months for entering cases into eTB Manager. As a result, even though the data for this analysis were downloaded from eTB Manager in November 2018, the number of notified adolescent TB cases in 2018 cannot be estimated with confidence. Of note, due to the lack of age- and sex-specific data on adolescent HIV prevalence in Ukraine, we could not stratify this analysis by HIV status.

We included 2491 incident TB cases among 2472 adolescents, of whom 19 (0.8%) contributed two cases each and therefore were considered twice in the cohort. Median age at treatment initiation was 17 years (interquartile range: 15–18). Sixty-nine of 2491 (2.8%) cases occurred in participants who received previous treatment for TB disease before the study period and/or before age 10 years. Among the 2385 cases with known HIV status, 88 (3.7%) were HIV-infected, of whom 52 (59.1%) were not on ART at TB treatment initiation. Table 1 further describes the cohort.

TABLE 1

Demographic and baseline clinical features of adolescent tuberculosis cases, Ukraine, 2015–2018 (n=2491)

	Ages 10–14 years	Ages 15–19 years	Ages 10–19 years
Subjects n (row %)	557 (22.4)	1934 (77.6)	2491 (100)
Sex
Female	280 (50.3)	901 (46.6)	1181 (47.4)
Male	277 (49.7)	1033 (53.4)	1310 (52.6)
HIV status
Infected, no ART start date documented	1 (0.2)	9 (0.5)	10 (0.4)
Infected, not on ART at start of TB treatment	29 (5.2)	23 (1.2)	52 (2.1)
Infected, on ART at start of TB treatment	13 (2.3)	13 (0.7)	26 (1.0)
Uninfected	479 (86.0)	1818 (94.0)	2297 (92.2)
Unknown	35 (6.3)	71 (3.6)	106 (4.3)
Urban or rural residence
Urban	292 (52.4)	1137 (58.8)	1429 (57.4)
Rural	262 (47.1)	784 (40.5)	1046 (42.0)
Unknown	3 (0.5)	13 (0.7)	16 (0.6)
Migration status
Migrant	6 (1.1)	26 (1.3)	32 (1.3)
Nonmigrant	551 (98.9)	1908 (98.7)	2469 (98.7)
Oblast
Conflict-affected	28 (5.0)	99 (5.1)	127 (5.1)
Not conflict-affected	529 (95.0)	1835 (94.9)	2364 (94.9)
History of alcohol use
Yes	0 (0.0)	17 (0.9)	17 (0.7)
No	557 (100.0)	1917 (99.1)	2474 (99.3)
History of incarceration
Yes	0 (0.0)	24 (1.2)	24 (1.0)
No	557 (100.0)	1910 (98.8)	2467 (99.0)
Probable source case identified
Yes	110 (19.7)	214 (11.1)	324 (13.0)
No	447 (80.3)	1720 (88.9)	2167 (87.0)
Case classification
New	537 (96.4)	1863 (96.3)	2400 (96.3)
Recurrent	20 (3.6)	71 (3.7)	91 (3.7)
Diagnosis
Microbiological	446 (80.1)	1756 (90.8)	2202 (88.4)
Clinical	111 (19.9)	178 (9.2)	289 (11.6)
Site of disease
Exclusively intrathoracic	455 (81.7)	1824 (94.3)	2279 (91.5)
Exclusively extrathoracic	85 (15.3)	66 (3.4)	151 (6.1)
Intra- and extrathoracic	17 (3.1)	44 (2.3)	61 (2.4)
Lung cavitation
Cavitary pulmonary TB	56 (10.1)	638 (33.0)	694 (27.9)
Noncavitary pulmonary TB	264 (47.4)	1076 (55.6)	1340 (53.8)
No pulmonary TB	237 (42.5)	220 (11.4)	457 (18.3)
Pleural TB
Isolated pleural	30 (5.4)	131 (6.8)	161 (6.5)
Pleural+pulmonary	12 (2.2)	45 (2.3)	57 (2.3)
No pleural involvement	515 (92.4)	1758 (90.9)	2273 (91.2)
Baseline drug resistance profile
INH- and RIF-susceptible	379 (68.0)	1289 (66.6)	1668 (67.0)
INH-monoresistant	11 (2.0)	108 (5.6)	119 (4.8)
RR but not pre-XDR	20 (3.6)	153 (0.8)	173 (6.8)
RR, no SLD DST	11 (2.0)	63 (3.3)	74 (3.0)
Pre-XDR but not XDR	12 (2.1)	73 (10.8)	85 (3.4)
XDR	6 (1.1)	38 (2.0)	44 (1.8)
No result#	118 (21.2)	210 (10.9)	328 (13.2)

Data are presented as n (column %), unless otherwise stated. ART: antiretroviral therapy; TB: tuberculosis; INH: isoniazid; RIF: rifampicin; RR: rifampicin-resistant; SLD: second-line drug; DST: drug susceptibility testing; XDR: extensively drug-resistant. #: DST result was not documented in 39 (1.8%) of 2202 microbiologically confirmed cases.

Of 2340 cases with intrathoracic involvement, 1900 (81.2%) involved lungs only; 160 (6.9%) involved pleura only; 145 (6.2%) involved intrathoracic lymph nodes only; 58 (2.5%) involved lungs and an extrathoracic site; 57 (2.4%) involved lungs and pleura; 17 (0.7%) involved lungs and intrathoracic lymph nodes; and three (0.1%) involved intrathoracic lymph nodes and an extrathoracic site. There were no cases of pericardial TB. Figure 2 shows the distribution of extrathoracic sites in the 212 cases with extrathoracic involvement.

FIGURE 2

Frequency of extrathoracic sites involved in a) exclusively extrathoracic adolescent tuberculosis cases (n=151) and b) cases with both intra- and extrathoracic involvement (n=61), Ukraine, 2015–2018. There are no data on the site from which the specimen was obtained for microbiological confirmation (i.e. specimen could have been obtained from the respiratory tract or the extrathoracic site). Only one reported case involved >1 extrathoracic site, the abdomen and bones and/or joints.

Tables 2–4 show the patient characteristics associated with pleural, extrathoracic and cavitary TB. Among 10–14-year-olds, we observed more pleural disease in boys than girls (adjusted OR (aOR) 2.12, 95% CI: 1.08–4.37). Pleural TB was inversely associated with RR-TB (aOR 0.44, 95% CI: 0.25–0.74). Extrathoracic TB was less common among 15–19-year-olds (aOR 0.26, 95% CI: 0.18–0.37) and more common among HIV-infected participants (aOR 3.25, 95% CI: 1.55–6.61 in 10–14-year-olds; aOR 8.18, 95% CI: 3.58–17.31 in 15–19-year-olds). Cavitary TB was associated with age 15–19 years (aOR 4.10, 95% CI: 3.00–5.73) and, in 15–19-year-olds only, inversely associated with HIV (aOR 0.32, 95% CI 0.13–0.70). We also observed higher odds of cavitation in migrants (aOR 3.53, 95% CI: 1.66, 7.61) and participants who reported excess alcohol use (aOR 3.29, 95% CI: 1.20, 9.52).

TABLE 2

Predictors of pleural disease among all tuberculosis cases (n=2491)

	n (% of subgroup)	Unadjusted OR (95% CI)	p-value	Adjusted OR (95% CI)	p-value
Age group
10–14 years	42 (7.5)	Ref
15–19 years	176 (9.1)	1.26 (0.89, 1.82)	0.20
Sex
Female (10–14 years)	14 (5.0)	Ref
Male (10–14 years)	28 (10.1)	2.14 (1.12, 4.27)	0.025	2.12 (1.08, 4.37)	0.034#
Female (15–19 years)	72 (8.0)	Ref
Male (15–19 years)	104 (10.1)	1.29 (0.94, 1.77)	0.14	1.29 (0.93, 1.80)	0.13
HIV status
Uninfected	206 (9.0)	Ref
Infected	3 (3.4)	0.36 (0.11, 1.14)	0.083	0.41 (0.10, 1.13)	0.14
Case classification
New	214 (8.9)	Ref
Recurrent	3 (3.3)	0.35 (0.11, 1.11)	0.074	0.38 (0.09, 1.03)	0.10
Oblast
Not conflict-affected	208 (8.8)	Ref
Conflict-affected	9 (7.1)	0.79 (0.37, 1.49)	0.51
Home setting
Suburban/rural	101 (9.0)	Ref
Urban	115 (8.0)	0.81 (0.61, 1.07)	0.14	0.89 (0.67, 1.19)	0.44
Migration status
Nonmigrant	218 (8.9)	Ref
Migrant	0 (0.0)	N/A
History of incarceration
No	217 (8.8)	Ref
Yes	0 (0.0)	N/A
Excess alcohol use
No	216 (8.7)	Ref
Yes	1 (5.9)	0.65 (0.04, 3.22)	0.68
Baseline rifampicin susceptibility
Susceptible	153 (8.6)	Ref
Resistant	15 (4.0)	0.44 (0.25, 0.74)	0.003	0.44 (0.25, 0.74)	0.003#

Only significant interactions are reported. N/A: not available. #: statistically significant using a cut-off of p<0.05.

TABLE 4

Predictors of cavitary pulmonary tuberculosis among all tuberculosis cases (n=2491)

	n (% of subgroup)	Unadjusted OR (95% CI)	p-value	Adjusted OR (95% CI)	p-value
Age group
10–14 years	56 (10.1)	Ref
15–19 years	638 (33.0)	4.40 (3.32, 5.95)	<0.001	4.10 (3.00, 5.73)	<0.001#
Sex
Female	313 (26.5)	Ref
Male	381 (29.1)	1.14 (0.95, 1.36)	0.15	1.11 (0.91, 1.34)	0.30
HIV status
Uninfected (10–14 years)	47 (9.8)	Ref
Infected (10–14 years)	7 (16.3)	1.79 (0.70, 4.02)	0.19	1.77 (0.67, 4.08)	0.21
Uninfected (15–19 years)	605 (33.3)	Ref
Infected (15–19 years)	8 (17.8)	0.43 (0.19, 0.89)	0.033	0.32 (0.13, 0.70)	0.007#
Case classification
New	661 (27.5)	Ref
Recurrent	33 (36.3)	1.50 (0.96, 2.30)	0.070	1.48 (0.91, 2.39)	0.11
Oblast
Not conflict-affected	655 (27.7)	Ref
Conflict-affected	39 (30.7)	1.16 (0.78, 1.69)	0.46
Home setting
Suburban/rural	288 (27.5)	Ref
Urban	403 (28.2)	1.03 (0.87, 1.24)	0.71
Migration status
Nonmigrant	677 (27.5)	Ref
Migrant	17 (53.1)	2.98 (1.48, 6.08)	0.002	3.53 (1.66, 7.61)	0.001#
History of incarceration
No	688 (27.9)	Ref
Yes	6 (25.0)	0.86 (0.31, 2.07)	0.75
Excess alcohol use
No	684 (27.6)	Ref
Yes	10 (58.8)	3.74 (1.43, 10.33)	0.008	3.29 (1.20, 9.52)	0.022#
Baseline rifampicin susceptibility
Susceptible	462 (25.9)	Ref
Resistant	197 (52.4)	3.16 (2.51, 3.97)	<0.001	2.98 (2.35, 3.78)	<0.001#

Only significant interactions are reported. #: statistically significant using a cut-off of p<0.05.

Of 2163 cases with a baseline DST result, the isolate was isoniazid- and rifampicin-susceptible in 1668 (77.1%); isoniazid-resistant but rifampicin-susceptible in 119 (7.1%); and RR in 376 (17.4%). Of the 302 RR-TB cases with second-line drug resistance profiles, 129 (42.7%) could be further classified as pre-XDR or XDR. At baseline, 16.9% of new and 29.7% of recurrent TB cases had confirmed rifampicin resistance. Stratified by age group, the percentages of RR-TB among new and recurrent cases, respectively, were 10.6% and 28.6% in younger adolescents and 18.6% and 30.0% in older adolescents. Table 5 shows the patient characteristics associated with RR-TB: recurrent TB (aOR 1.87, 95% CI: 1.08–3.13), urban residence (aOR 1.27, 95% CI: 1.01–1.62) and cavitary TB (aOR 2.98, 95% CI: 2.35–3.78). There were no significant interactions between new versus recurrent TB and the other predictors.

TABLE 5

Predictors of rifampicin-resistant tuberculosis among incident cases with baseline drug susceptibility test results (n=2163)

	n (% of subgroup)	Unadjusted OR (95% CI)	p-value	Adjusted OR (95% CI)	p-value
Age group
10–14 years	49 (11.2)	Ref
15–19 years	327 (19.0)	1.86 (1.36, 2.59)	<0.001	1.33 (0.96, 1.88)	0.094
Sex
Female	165 (15.9)	Ref
Male	211 (18.7)	1.22 (0.97, 1.52)	0.09	1.19 (0.95, 1.50)	0.14
HIV status
Uninfected	360 (17.9)	Ref
Infected	15 (20.0)	1.15 (0.62, 1.99)	0.64
Case classification
New	354 (16.9)	Ref
Recurrent	22 (29.7)	2.07 (1.22, 3.41)	0.005	1.87 (1.08, 3.13)	0.021#
Oblast
Not conflict-affected	355 (17.3)	Ref
Conflict-affected	21 (19.6)	1.17 (0.70, 1.87)	0.53
Home setting
Suburban/rural	139 (15.4)	Ref
Urban	234 (18.8)	1.27 (1.01, 1.61)	0.039	1.27 (1.01, 1.62)	0.047#
Migration status
Nonmigrant	369 (17.3)	Ref
Migrant	7 (25.9)	1.68 (0.65, 3.82)	0.24
History of incarceration
No	370 (17.3)	Ref
Yes	6 (26.1)	1.69 (0.61, 4.09)	0.27
Excess alcohol use
No	371 (17.3)	Ref
Yes	5 (35.7)	2.66 (0.81, 7.75)	0.08	2.07 (0.62, 6.26)	0.21
Cavitary tuberculosis
No	163 (12.5)	Ref
Yes	197 (30.0)	2.98 (2.36, 3.77)	<0.001	2.81 (2.21, 3.58)	<0.001#
Not applicable (no lung involvement)	16 (7.9)	0.60 (0.35, 1.03)	0.063	0.64 (0.36, 1.07)	0.11

Only significant interactions are reported. #: statistically significant using a cut-off of p<0.05.

Discussion

In this study, we have estimated independent associations between patient characteristics and clinical presentations of adolescent TB. We also have described the epidemiology of adolescent TB in Ukraine. Several of our results—such as the age- and sex-related patterns in lung cavitation and pleural TB—confirm previous descriptive reports, but our analysis adjusted for covariates [2-7]. In our cohort, the steepest rise in TB incidence in girls occurs right after the average age of menarche in Ukraine, which is 12.9 years [29]. These findings suggest interactions between pubertal hormones and host response to Mtb; other factors, including increased exposure to TB, may also contribute. Evidence for and against these and other explanations for age- and sex-related patterns in TB presentation have been extensively reviewed elsewhere [27].

To our knowledge, previous studies have not examined the impact of HIV coinfection, RR or social factors on adolescent TB presentation. In our cohort, two-thirds of HIV-infected participants with known ART start date had not received ART before TB diagnosis. This finding raises concern for gaps in adolescent HIV care, and means that HIV coinfection is a reasonable surrogate for immunosuppression in this group. The rise in cavitary TB incidence during adolescence is believed to be caused by the bolstering of host response to Mtb by pubertal hormones [27]. Our observation of an inverse association between HIV coinfection and lung cavitation in older adolescents—which mirrors findings from studies in adults—supports this hypothesis [30, 31]. The low number of 10–14-year-olds with cavitary TB may explain the lack of association between HIV and cavitation in the younger group. Unsurprisingly, in both age groups, HIV was associated with extrathoracic TB, which arises from progression or re-activation of distal sites of infection attributable to immunological failure to contain Mtb in the lungs.

We observed that compared to adolescents with rifampicin-susceptible TB, those with RR-TB had three-times the odds of lung cavitation at diagnosis. As resistance-conferring mutations in the Mtb genome occur at fixed frequencies, the higher bacillary load found in cavities predisposes to de novo resistance [32]. Another possible explanation for this observation is that Mtb genotype is a confounder associated with both cavitary disease and RR-TB. A study from Russia found an association between radiographically advanced disease, including widespread cavitation and Beijing-family strains—which are associated with RR-TB [33]. Studies from other locations did not find an association between Beijing-family strains and cavitation [34, 35]; however, Beijing subtypes in former Soviet republics may have a different predilection for causing cavities or another Mtb strain may be responsible. Because eTB Manager does not include data on genotype, we could not assess its relationship with clinical presentation. We also found that participants with baseline RR-TB had half the odds of pleural TB. The same hypotheses may explain this inverse association: because pleural TB is paucibacillary, de novo resistance may be less likely to emerge or Mtb genotype may be a confounder.

We observed associations between social factors and RR-TB and cavitary TB. Urban residents had higher odds of RR-TB compared to rural residents. Previous studies have reported this association among adults in Ukraine [36, 37]. Therefore, compared to adolescents living in rural areas, those in urban areas have a higher risk of exposure to RR-TB. In our cohort, migrants and excess alcohol users had increased odds of cavitary TB; however, these results should be interpreted with caution, given the limitations in how migration and excess alcohol use were reported. In Ukraine, both migrants and excess alcohol users face healthcare barriers [38-40]. Treatment delay, which has been associated with lung cavitation in adults with TB, may explain our finding [41, 42]. It is also possible that alcohol—or another factor associated with alcohol use, such as cigarette smoking—alters host response to Mtb in a way that predisposes to cavitation. Further investigation is needed to confirm and elucidate the relationships that we observed between social factors and severe forms of TB.

Only a few publications have reported the burden of RR-TB in adolescence [12, 43]. In our cohort, 17% of new adolescent TB cases had RR-TB, compared to 29% reported for all ages [16]. We are unsure of the reasons behind this difference in prevalence, particularly because the national drug resistance survey reported higher adjusted odds of RR-TB among 14–24-year-olds than in older age groups [14]. One potential explanation is that the finding in the national survey was driven by young adults 20–24 years of age. In Ukraine, the number of HIV infections is seven-times higher in 20–24-year-olds than in 10–19-year-olds, and HIV is an independent risk factor for RR-TB [14, 44, 45]. Another possibility is that compared to young adults, adolescents have less exposure to groups with more RR-TB—including not only HIV-infected people but also drug users and individuals with a history of incarceration. As a result, they are at lower risk for becoming infected with RR Mtb strains. These hypotheses warrant further investigation.

There was an even larger gap between the 30% of RR-TB among recurrent cases in our cohort and the 46% reported for all ages, but additional factors may have contributed to this difference [16]. First, the previously treated cases reported among all ages included patients who started a new regimen after the previous regimen ended in treatment failure or loss to follow-up. In contrast, our definition of recurrent TB did not include these patients, who are more likely to have RR-TB. Second, the drug resistance survey showed that, among previously treated patients, 35–54-year-olds had a higher risk of RR-TB than 15–24-year-olds [14]. Therefore, the higher prevalence of RR-TB in the general population likely is driven by older age groups.

This study has limitations. First, due to the lack of HIV viral load and CD4 count data, we could not further characterise the immunosuppression of HIV-infected participants and its relationship with clinical presentation. Second, excess alcohol use and migrant status were self-reported and data fields for these variables were completed only when positive. This limitation likely led to an underestimation of the prevalence of these factors. Third, as in any study that uses programmatic data, clerical errors may have occurred. We excluded TB cases reported before 2015 (during the eTB Manager roll-out period) to reduce this possibility. Fourth, the high proportion of microbiologically confirmed cases raises concern for the underdiagnosis of paucibacillary disease, including childhood-type pulmonary TB and extrathoracic TB. Last, the lack of NTP guidelines on extrapulmonary TB diagnosis and limited access to sampling procedures and advanced imaging may have contributed to misdiagnoses.

Despite these limitations, we have identified independent associations between patient characteristics and clinical manifestations of TB in a large adolescent cohort. These findings inform clinicians evaluating adolescents for TB, provide clues to TB pathophysiology and suggest targets for intervention to improve outcomes.

TABLE 3

Predictors of extrathoracic involvement among all tuberculosis cases (n=2491)

	n (% of subgroup)	Unadjusted OR (95% CI)	p-value	Adjusted OR (95% CI)	p-value
Age group
10–14 years	99 (17.8)	Ref
15–19 years	91 (4.7)	0.23 (0.17, 0.31)	<0.001	0.26 (0.18, 0.37)	<0.001#
Sex
Female	84 (7.1)	Ref
Male	106 (8.1)	1.15 (0.85, 1.55)	0.36
HIV status
Uninfected (10–14 years)	79 (16.5)	Ref
Infected (10–14 years)	15 (34.9)	2.71 (1.36, 5.25)	0.004	3.25 (1.55, 6.61)	<0.001#
Uninfected (15–19 years)	76 (4.2)	Ref
Infected (15–19 years)	10 (22.2)	6.55 (2.98, 13.26)	<0.001	8.18 (3.58, 17.31)	<0.001#
Case classification
New	182 (7.6)	Ref
Recurrent	8 (8.8)	1.17 (0.52, 2.32)	0.67
Oblast
Not conflict-affected	186 (7.9)	Ref
Conflict-affected	4 (3.1)	0.38 (0.14, 1.04)	0.060	0.36 (0.12, 1.09)	0.070
Home setting
Suburban/rural	87 (8.3)	Ref
Urban	102 (7.1)	0.85 (0.63, 1.14)	0.28
Migration status
Nonmigrant	190 (7.7)	Ref
Migrant	0 (0.0)	NA	NA
History of incarceration
No	189 (7.7)	Ref
Yes	1 (4.2)	0.52 (0.03, 2.51)	0.53
Excess alcohol use
No	188 (7.6)	Ref
Yes	2 (11.8)	1.62 (0.25, 5.80)	0.52
Baseline rifampicin susceptibility
Susceptible	98 (5.5)	Ref
Resistant	12 (3.2)	0.57 (0.29, 1.00)	0.069	0.64 (0.32, 1.14)	0.16

Only significant interactions are reported. #: statistically significant using a cut-off of p<0.05.