| Literature DB >> 32959243 |
Julie Harnischfeger1, Lukas Käßer1, Jan Zitzmann1, Denise Salzig1, Peter Czermak2,3,4.
Abstract
Several vaccines are already produced using the baculovirus expression vector system (BEVS). This chapter describes methods for generating recombinant baculoviral DNA (also called bacmid) for cultivating Spodoptera frugiperda Sf-9 cells and producing a baculovirus stock from the recombinant bacmid and for producing a protein-based vaccine with the BEVS in a stirred tank reactor.Entities:
Keywords: Antigen production; Cell cultivation; Dielectric spectroscopy; Online process monitoring; Process analytical technology; Sf-9 cells; Spodoptera frugiperda
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Year: 2021 PMID： 32959243 DOI： 10.1007/978-1-0716-0795-4_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745