Anna Díez-Villanueva1,2,3, Rebeca Sanz-Pamplona1,2,3, Robert Carreras-Torres1,2,3, Ferran Moratalla-Navarro1,2,3,4, M Henar Alonso1,2,3,4, Laia Paré-Brunet1,2, Susanna Aussó1,2, Elisabet Guinó1,2,3, Xavier Solé1,2,3, David Cordero1,2,3, Ramón Salazar2,5,6, Maria Berdasco7,8, Miguel A Peinado9, Victor Moreno1,2,3,4. 1. Unit of Biomarkers & Susceptibility, Oncology Data Analytics Program, Catalan Institute of Oncology (ICO), 08908 Hospitalet de Llobregat, Barcelona, Spain. 2. Colorectal Cancer Group, ONCOBELL Program, Bellvitge Biomedical Research Institute (IDIBELL), 08908 Hospitalet de Llobregat, Barcelona, Spain. 3. Biomedical Research Centre Network for Epidemiology & Public Health (CIBERESP), 28029 Madrid, Spain. 4. Department of Clinical Sciences, Faculty of Medicine and Health Sciences, University of Barcelona, 08907 Barcelona, Spain. 5. Biomedical Research Centre Network for Cancer (CIBERONC), 28029 Madrid, Spain. 6. Medical Oncology Service, Catalan Institute of Oncology (ICO), 08908 Hospitalet de Llobregat, Barcelona, Spain. 7. Cancer Epigenetics & Biology Program, Bellvitge Biomedical Research Institute (IDIBELL), 08908 Hospitalet de Llobregat, Barcelona, Spain. 8. Epigenetic Therapies Group, Experimental & Clinical Hematology Program (PHEC), Josep Carreras Leukaemia Research Institute, 08916 Badalona, Barcelona, Spain. 9. Program of Predictive and Personalized Medicine of Cancer, Germans Trias i Pujol Research Institute (PMPPC-IGTP), 08916 Badalona, Barcelona, Spain.
Abstract
Aim: Gain insight about the role of DNA methylation in the malignant growth of colon cancer. Patients & methods: Methylation and gene expression from 90 adjacent-tumor paired tissues and 48 healthy tissues were analyzed. Tumor genes whose change in expression was explained by changes in methylation were identified using linear models adjusted for tumor stromal content. Results: No differences in methylation were found between adjacent and healthy tissues, but clear differences were found between adjacent and tumor samples. We identified hypermethylated CpG islands located in promoter regions that drive differential gene expression of transcription factors and their target genes. Conclusion: Changes in methylation of a few genes provoke important changes in gene expression, by expanding the signal through transcription activation/repression.
Aim: Gain insight about the role of DNA methylation in the malignant growth of colon cancer. Patients & methods: Methylation and gene expression from 90 adjacent-tumor paired tissues and 48 healthy tissues were analyzed. Tumor genes whose change in expression was explained by changes in methylation were identified using linear models adjusted for tumor stromal content. Results: No differences in methylation were found between adjacent and healthy tissues, but clear differences were found between adjacent and tumor samples. We identified hypermethylated CpG islands located in promoter regions that drive differential gene expression of transcription factors and their target genes. Conclusion: Changes in methylation of a few genes provoke important changes in gene expression, by expanding the signal through transcription activation/repression.
Authors: Matthew Devall; Xiangqing Sun; Fangcheng Yuan; Gregory S Cooper; Joseph Willis; Daniel J Weisenberger; Graham Casey; Li Li Journal: J Natl Cancer Inst Date: 2020-12-30 Impact factor: 13.506
Authors: Sine Knorr; Anne Skakkebæk; Jesper Just; Emma B Johannsen; Christian Trolle; Søren Vang; Zuzana Lohse; Birgitte Bytoft; Peter Damm; Kurt Højlund; Dorte M Jensen; Claus H Gravholt Journal: BMC Med Date: 2022-09-23 Impact factor: 11.150
Authors: Anna Díez-Villanueva; Rebeca Sanz-Pamplona; Xavier Solé; David Cordero; Marta Crous-Bou; Elisabet Guinó; Adriana Lopez-Doriga; Antoni Berenguer; Susanna Aussó; Laia Paré-Brunet; Mireia Obón-Santacana; Ferran Moratalla-Navarro; Ramon Salazar; Xavier Sanjuan; Cristina Santos; Sebastiano Biondo; Virginia Diez-Obrero; Ainhoa Garcia-Serrano; Maria Henar Alonso; Robert Carreras-Torres; Adria Closa; Víctor Moreno Journal: Sci Data Date: 2022-10-01 Impact factor: 8.501