Qiong Wang1, François Guerrero1, Michaël Theron1,2. 1. Laboratory ORPHY, European University of Bretagne, University of Brest, Brest, France. 2. Corresponding author: Dr Michaël Theron, Laboratory ORPHY, European University of Bretagne, University of Brest, 6 Avenue Le Gorgeu, 29238 Brest, France, michael.theron@univ-brest.fr.
Abstract
INTRODUCTION: Hydration status is considered a parameter likely to influence the risk of decompression sickness (DCS), but scientific evidence is scarce and conflicting. This experiment aimed to analyse the influence of pre-hydration on DCS occurrence in a rat model. METHODS: Intra-peritoneal injections of saline solution were administered to rats (NaCl 0.9% 0 ml (Control), 0.1 ml (Group 1), or 1 ml·100g-1 body mass (Group 2) at each of 24 h, 12 h, and 30 min prior to simulated air dives (45 min at 1,010 kPa; compression and decompression rates 101 kPa·min-1; stops 5 min at 202 kPa, 5 min at 160 kPa, 10 min at 130 kPa). Evaluation of DCS occurrence and severity was made after decompression. RESULTS: Pre-dive hydration reduced severe DCS from 47% (Control) to 29% (Group 1) and 0% (Group 2), and increased the proportion of animals without any signs of DCS from 40 (Control) to 57% (Group 1) and 93% (Group 2); Chi2 P = 0.041. CONCLUSIONS: This experiment demonstrated that pre-hydration can drastically reduce the DCS occurrence in an animal model. In the context of scuba diving, this result highlights the importance of elucidating the mechanisms linking hydration status and DCS risk. Copyright: This article is the copyright of the authors who grant Diving and Hyperbaric Medicine a non-exclusive licence to publish the article in electronic and other forms.
INTRODUCTION: Hydration status is considered a parameter likely to influence the risk of decompression sickness (DCS), but scientific evidence is scarce and conflicting. This experiment aimed to analyse the influence of pre-hydration on DCS occurrence in a rat model. METHODS: Intra-peritoneal injections of saline solution were administered to rats (NaCl 0.9% 0 ml (Control), 0.1 ml (Group 1), or 1 ml·100g-1 body mass (Group 2) at each of 24 h, 12 h, and 30 min prior to simulated air dives (45 min at 1,010 kPa; compression and decompression rates 101 kPa·min-1; stops 5 min at 202 kPa, 5 min at 160 kPa, 10 min at 130 kPa). Evaluation of DCS occurrence and severity was made after decompression. RESULTS: Pre-dive hydration reduced severe DCS from 47% (Control) to 29% (Group 1) and 0% (Group 2), and increased the proportion of animals without any signs of DCS from 40 (Control) to 57% (Group 1) and 93% (Group 2); Chi2 P = 0.041. CONCLUSIONS: This experiment demonstrated that pre-hydration can drastically reduce the DCS occurrence in an animal model. In the context of scuba diving, this result highlights the importance of elucidating the mechanisms linking hydration status and DCS risk. Copyright: This article is the copyright of the authors who grant Diving and Hyperbaric Medicine a non-exclusive licence to publish the article in electronic and other forms.
Entities:
Keywords:
Animal model; Diving deaths; Hydration; Rat
Authors: Qiong Wang; Aleksandra Mazur; François Guerrero; Kate Lambrechts; Peter Buzzacott; Marc Belhomme; Michaël Theron Journal: J Appl Physiol (1985) Date: 2015-10-15
Authors: Peter Buzzacott; Kate Lambrechts; Aleksandra Mazur; Qiong Wang; Virginie Papadopoulou; Michael Theron; Costantino Balestra; François Guerrero Journal: Comput Biol Med Date: 2014-10-22 Impact factor: 4.589